Search Results - "Schiro, Perry"

Bioconjugation of Ultrabright Semiconducting Polymer Dots for Specific Cellular Targeting by Wu, Changfeng, Schneider, Thomas, Zeigler, Maxwell, Yu, Jiangbo, Schiro, Perry G, Burnham, Daniel R, McNeill, Jason D, Chiu, Daniel T

“…Semiconducting polymer dots (Pdots) represent a new class of ultrabright fluorescent probes for biological imaging. They exhibit several important…”
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Sequential Ensemble-Decision Aliquot Ranking Isolation and Fluorescence In Situ Hybridization Identification of Rare Cells from Blood by Using Concentrated Peripheral Blood Mononuclear Cells by Xu, Shihan, Wu, Li, Qin, Yuling, Jiang, Yifei, Sun, Kai, Holcomb, Chenee, Gravett, Michael G, Vojtech, Lucia, Schiro, Perry G, Chiu, Daniel T

“…Isolation and analysis of circulating rare cells is a promising approach for early detection of cancer and other diseases and for prenatal diagnosis. Isolation…”
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Sensitive and High-Throughput Isolation of Rare Cells from Peripheral Blood with Ensemble-Decision Aliquot Ranking by Schiro, Perry G., Zhao, Mengxia, Kuo, Jason S., Koehler, Karen M., Sabath, Daniel E., Chiu, Daniel T.

“…Enrichment of cells: An approach called ensemble‐decision aliquot ranking (eDAR) for isolating rare cells from peripheral blood is described. The eDAR process…”
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Protein quantification at the single vesicle level reveals that a subset of synaptic vesicle proteins are trafficked with high precision by Mutch, Sarah A, Kensel-Hammes, Patricia, Gadd, Jennifer C, Fujimoto, Bryant S, Allen, Richard W, Schiro, Perry G, Lorenz, Robert M, Kuyper, Christopher L, Kuo, Jason S, Bajjalieh, Sandra M, Chiu, Daniel T

“…Protein sorting represents a potential point of regulation in neurotransmission because it dictates the protein composition of synaptic vesicles, the organelle…”
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Tracking B Cell Memory to SARS-CoV-2 Using Rare Cell Analysis System by Tsai, Dong-Yan, Wang, Chun-Hung, Schiro, Perry G, Chen, Nathan, Tseng, Ju-Yu

“…Rapid mutations within SARS-CoV-2 are driving immune escape, highlighting the need for in-depth and routine analysis of memory B cells (MBCs) to complement the…”
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An Automated High-Throughput Counting Method for Screening Circulating Tumor Cells in Peripheral Blood by Zhao, Mengxia, Schiro, Perry G, Kuo, Jason S, Koehler, Karen M, Sabath, Daniel E, Popov, Viorica, Feng, Qinghua, Chiu, Daniel T

“…Enumeration of circulating tumor cells (CTCs) has proved valuable for early detection and prognosis in cancer treatment. This paper describes an automated…”
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Deformability considerations in filtration of biological cells by Kuo, Jason S, Zhao, Yongxi, Schiro, Perry G, Ng, Laiying, Lim, David S W, Shelby, J Patrick, Chiu, Daniel T

“…Biological cells are highly sensitive to variation in local pressure because cellular membranes are not rigid. Unlike microbeads, cells deform under pressure…”
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Isolating Rare Cells and Circulating Tumor Cells with High Purity by Sequential eDAR by Johnson, Eleanor S, Xu, Shihan, Yu, Hui-Min, Fang, Wei-Feng, Qin, Yuling, Wu, Li, Wang, Jiasi, Zhao, Mengxia, Schiro, Perry G, Fujimoto, Bryant, Chen, Jui-Lin, Chiu, Daniel T

“…Isolation and analysis of circulating tumor cells (CTCs) from the blood of patients at risk of metastatic cancers is a promising approach to improving cancer…”
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MiSelect R System: the validation of a new detection system of CTCs and their correlation with prognosis in non-metastatic CRC patients by Lin, Chun-Chi, Yang, Chih-Yung, Hung, Tzu-Chao, Wang, Chun-Hung, Wei, Sheng-Wen, Schiro, Perry, Tseng, Ju-Yu, Lin, Chi-Hung, Jiang, Jeng-Kai

“…Circulating tumor cells (CTCs) in blood are accepted as a prognostic marker for patients with metastatic colorectal cancer (CRC). However, there is limited…”
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Simultaneous and selective isolation of multiple subpopulations of rare cells from peripheral blood using ensemble-decision aliquot ranking (eDAR) by Zhao, Mengxia, Wei, Bingchuan, Nelson, Wyatt C, Schiro, Perry G, Chiu, Daniel T

“…Rare cells, such as circulating tumor cells (CTCs), can be heterogeneous. The isolation and identification of rare cells with different phenotypes is…”
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Determining the number of specific proteins in cellular compartments by quantitative microscopy by Mutch, Sarah A, Gadd, Jennifer C, Fujimoto, Bryant S, Kensel-Hammes, Patricia, Schiro, Perry G, Bajjalieh, Sandra M, Chiu, Daniel T

“…This protocol describes a method for determining both the average number and variance of proteins, in the few to tens of copies, in isolated cellular…”
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New Generation of Ensemble-Decision Aliquot Ranking Based on Simplified Microfluidic Components for Large-Capacity Trapping of Circulating Tumor Cells by Zhao, Mengxia, Nelson, Wyatt C., Wei, Bingchuan, Schiro, Perry G., Hakimi, Bejan M., Johnson, Eleanor S., Anand, Robbyn K., Gyurkey, Grace S., White, Lisa M., Whiting, Samuel H., Coveler, Andrew L., Chiu, Daniel T.

“…Ensemble-decision aliquot ranking (eDAR) is a sensitive and high-throughput method to analyze circulating tumor cells (CTCs) from peripheral blood. Here, we…”
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High-Throughput Fluorescence-Activated Nanoscale Subcellular Sorter with Single-Molecule Sensitivity by Schiro, Perry G, Gadd, Jennifer C, Yen, Gloria S, Chiu, Daniel T

“…Recent single-cell and single-molecule studies have shown that a variety of subpopulations exist within biological systems, such as synaptic vesicles, that…”
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Continuous-flow single-molecule CE with high detection efficiency by Schiro, Perry G., Kuyper, Christopher L., Chiu, Daniel T.

“…This paper describes the use of two‐beam line‐confocal detection geometry for measuring the total mobility of individual molecules undergoing continuous‐flow…”
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Accurate Sizing of Nanoparticles Using Confocal Correlation Spectroscopy by Kuyper, Christopher L, Fujimoto, Bryant S, Zhao, Yiqiong, Schiro, Perry G, Chiu, Daniel T

“…The ability to accurately size low concentrations of nanoscale particles in small volumes is useful for a broad range of disciplines. Here, we characterize…”
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Optical gradient flow focusing by Zhao, Yiqiong, Fujimoto, Bryant S, Jeffries, Gavin D, Schiro, Perry G, Chiu, Daniel T

“…This paper describes a new method for carrying out flow cytometry, which employs optical gradient forces to guide and focus particles in the fluid flow. An…”
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A new USP Class VI-compliant substrate for manufacturing disposable microfluidic devices by Kuo, Jason S, Ng, Laiying, Yen, Gloria S, Lorenz, Robert M, Schiro, Perry G, Edgar, J Scott, Zhao, Yongxi, Lim, David S W, Allen, Peter B, Jeffries, Gavin D M, Chiu, Daniel T

“…As microfluidic systems transition from research tools to disposable clinical-diagnostic devices, new substrate materials are needed to meet both the…”
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Method for the Accurate Preparation of Cell-Spiking Standards by Zhao, Yongxi, Schiro, Perry G, Kuo, Jason S, Ng, Laiying, Chiu, Daniel T

“…Preparation of calibration standards for cell enumeration is critical in characterizing the performance of any method or apparatus intended for recovering rare…”
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Cavity-enhanced emission from a dye-coated microsphere by Schiro, Perry, Kwok, Alfred

“…We have observed whispering gallery modes in the inelastic emission from a 9.8 microm polystyrene bead coated with a monolayer of AlexaFluor 488 dye. Using a…”
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Fabrication improvements for thermoset polyester (TPE) microfluidic devices by Fiorini, Gina S, Yim, Moonbin, Jeffries, Gavin D M, Schiro, Perry G, Mutch, Sarah A, Lorenz, Robert M, Chiu, Daniel T

“…Thermoset polyester (TPE) microfluidic devices were previously developed as an alternative to poly(dimethylsiloxane) (PDMS) devices, fabricated similarly by…”
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