Regulation of laccase synthesis in induced Neurospora crassa cultures

Rapidly growing cultures of N. crassa do not produce laccase. Exposure of this fungus to different inducing agents leads to a de novo biosynthesis of extracellular laccase in vegetative cultures. In this study the induction of laccase after addition of cycloheximide and D-phenylalanine is reported....

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Bibliographic Details
Published in:	Current genetics Vol. 19; no. 5; pp. 375 - 381
Main Authors:	Linden, R.M. (Zuerich Univ. (Switzerland). Biochemisches Inst.), Schilling, B.C, Germann, U.A, Lerch, K
Format:	Journal Article
Language:	English
Published:	Heidelberg Springer 01-05-1991 Berlin
Subjects:	Biological and medical sciences BIOSINTESIS BIOSYNTHESE BIOSYNTHESIS Blotting, Western CICLOHEXIMIDA Classical genetics, quantitative genetics, hybrids CYCLOHEXIMIDE Cycloheximide - pharmacology D-phenylalanine Enzyme Induction FENILALANINA Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Enzymologic Gene Expression Regulation, Fungal Genetics of eukaryotes. Biological and molecular evolution Kinetics LACASA LACCASE NEUROSPORA Neurospora crassa Neurospora crassa - enzymology Oxidoreductases - biosynthesis Oxidoreductases - genetics PHENYLALANINE PHYSIOLOGICAL REGULATION REGULACION FISIOLOGICA REGULATION PHYSIOLOGIQUE RNA, Fungal - genetics RNA, Messenger - genetics Thallophyta, bryophyta Vegetals Phenylalanine Enzyme Induction Gene product Tissue culture Biosynthesis Gene expression Fungi Regulation(control) Ascomycetes Neurospora crassa Thallophyta Laccase
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Summary:	Rapidly growing cultures of N. crassa do not produce laccase. Exposure of this fungus to different inducing agents leads to a de novo biosynthesis of extracellular laccase in vegetative cultures. In this study the induction of laccase after addition of cycloheximide and D-phenylalanine is reported. De novo synthesis of laccase mRNA was followed over 96 h after induction. A fast appearance of the message, as well as its presence over a rather long period, indicates a regulation on a transcriptional and maybe on a post-transcriptional level. In contrast to the kinetics of mRNA production, Western analysis with a polyclonal anti-laccase antibody showed a remarkably delayed appearance of the intracellular, as well as of the extracellular, protein product after induction with cycloheximide. Furthermore, activity measurements at different times after induction of both crude extracts and media of the vegetative cultures showed that in extracted mycelia the activity occurs at least 20 h after the protein is immunologically detectable. Laccase activity in the medium starts to increase only 30 h after translation. These data, together with the published structure of the laccase gene, indicate a regulation on the transcriptional, post-transcriptional and on a post-translational level. In cultures induced with D-phenylalanine a rather fast appearance of laccase-specific mRNA also indicates a transcriptional regulation. Compared to cycloheximide-induced laccase biosynthesis no delayed appearance of laccase protein levels of laccase activity is observed after induction with D-phenylalanine.
Bibliography:	93B0113 F30 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0172-8083 1432-0983
DOI:	10.1007/BF00309598