Search Results - "Scheller, Silvan"
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Artificial electron acceptors decouple archaeal methane oxidation from sulfate reduction
Published in Science (American Association for the Advancement of Science) (12-02-2016)“…The oxidation of methane with sulfate is an important microbial metabolism in the global carbon cycle. In marine methane seeps, this process is mediated by…”
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Microbial Interconversion of Alkanes to Electricity
Published in Frontiers in energy research (05-11-2018)“…Electricity from fuels can be produced via 2 fundamentally different methods: By burning them to spin generators, or by direct abstraction of electrons at…”
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CRISPR/Cas12a toolbox for genome editing in Methanosarcina acetivorans
Published in Frontiers in microbiology (12-12-2023)“…Methanogenic archaea play an important role in the global carbon cycle and may serve as host organisms for the biotechnological production of fuels and…”
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In situ visualization of newly synthesized proteins in environmental microbes using amino acid tagging and click chemistry
Published in Environmental microbiology (01-08-2014)“…Here we describe the application of a new click chemistry method for fluorescent tracking of protein synthesis in individual microorganisms within…”
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Comparative analysis of CO2 reduction by soluble Escherichia coli formate dehydrogenase H and its selenocysteine-to-cysteine substitution variant
Published in Journal of CO2 utilization (01-11-2023)“…Metal-dependent formate dehydrogenases (Me-FDHs) are highly active CO2-reducing enzymes operating at low redox potentials and employ either molybdenum or…”
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Methyl-Coenzyme M Reductase from Methanogenic Archaea: Isotope Effects on the Formation and Anaerobic Oxidation of Methane
Published in Journal of the American Chemical Society (09-10-2013)“…The nickel enzyme methyl-coenzyme M reductase (MCR) catalyzes two important transformations in the global carbon cycle: methane formation and its reverse, the…”
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Comparative Genomics and Proteomic Analysis of Assimilatory Sulfate Reduction Pathways in Anaerobic Methanotrophic Archaea
Published in Frontiers in microbiology (03-12-2018)“…Sulfate is the predominant electron acceptor for anaerobic oxidation of methane (AOM) in marine sediments. This process is carried out by a syntrophic…”
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Monodeuterated Methane, an Isotopic Tool To Assess Biological Methane Metabolism Rates
Published in mSphere (01-07-2017)“…Biological methane oxidation is a globally relevant process that mediates the flux of an important greenhouse gas through both aerobic and anaerobic metabolic…”
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Application of the Fluorescence-Activating and Absorption-Shifting Tag (FAST) for Flow Cytometry in Methanogenic Archaea
Published in Applied and environmental microbiology (26-04-2023)“…Methane-producing archaea play a crucial role in the global carbon cycle and are used for biotechnological fuel production. Methanogenic model organisms such…”
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A growth-based screening strategy for engineering the catalytic activity of an oxygen-sensitive formate dehydrogenase
Published in Applied and environmental microbiology (18-09-2024)“…Enzyme engineering is a powerful tool for improving or altering the properties of biocatalysts for industrial, research, and therapeutic applications. Fast and…”
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The key nickel enzyme of methanogenesis catalyses the anaerobic oxidation of methane
Published in Nature (03-06-2010)“…Large amounts (estimates range from 70 Tg per year to 300 Tg per year) of the potent greenhouse gas methane are oxidized to carbon dioxide in marine sediments…”
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A promoter-RBS library for fine-tuning gene expression in Methanosarcina acetivorans
Published in Applied and environmental microbiology (18-09-2024)“…Methanogens are the main biological producers of methane on Earth. is one of the best characterized methanogens that has powerful genetic tools for genome…”
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Thiol‐Disulfide Exchange Kinetics and Redox Potential of the Coenzyme M and Coenzyme B Heterodisulfide, an Electron Acceptor Coupled to Energy Conservation in Methanogenic Archaea
Published in Chembiochem : a European journal of chemical biology (02-01-2024)“…Methanogenic and methanotrophic archaea play important roles in the global carbon cycle by interconverting CO2 and methane. To conserve energy from these…”
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Deconstructing Methanosarcina acetivorans into an acetogenic archaeon
Published in Proceedings of the National Academy of Sciences - PNAS (11-01-2022)“…The reductive acetyl-coenzyme A (acetyl-CoA) pathway, whereby carbon dioxide is sequentially reduced to acetyl-CoA via coenzyme-bound C1 intermediates, is the…”
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Efficient CRISPR/Cas12a-Based Genome-Editing Toolbox for Metabolic Engineering in Methanococcus maripaludis
Published in ACS synthetic biology (15-07-2022)“…The rapid-growing and genetically tractable methanogen Methanococcus maripaludis is a promising host organism for the biotechnological conversion of carbon…”
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Realities of the consortium approach in science: sustainable enzymatic production of C1 chemicals from carbon dioxide
Published in RSC sustainability (31-10-2024)“…Research at the frontiers of science is getting increasingly specialised. At the same time, major global challenges require the cooperation and innovation of…”
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Methyl-Coenzyme M Reductase from Methanogenic Archaea: Isotope Effects on Label Exchange and Ethane Formation with the Homologous Substrate Ethyl-Coenzyme M
Published in Journal of the American Chemical Society (09-10-2013)“…Ethyl-coenzyme M (CH3CH2-S-CH2CH2-SO3 –, Et-S-CoM) serves as a homologous substrate for the enzyme methyl-coenzyme M reductase (MCR) resulting in the product…”
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Intermediates in the Catalytic Cycle of Methyl Coenzyme M Reductase: Isotope Exchange is Consistent with Formation of a σ‐Alkane-Nickel Complex
Published in Angewandte Chemie (International ed.) (25-10-2010)“…The key nickel enzyme for methanogenesis (MCR) catalyzes the formation of CH3D and CH2D2 in a deuterated medium. CH2D2 is formed by an exchange of deuterium…”
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Advanced electron paramagnetic resonance on the catalytic iron–sulfur cluster bound to the CCG domain of heterodisulfide reductase and succinate: quinone reductase
Published in Journal of biological inorganic chemistry (01-12-2013)“…Heterodisulfide reductase (Hdr) is a key enzyme in the energy metabolism of methanogenic archaea. The enzyme catalyzes the reversible reduction of the…”
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Stable Isotope Phenotyping via Cluster Analysis of NanoSIMS Data As a Method for Characterizing Distinct Microbial Ecophysiologies and Sulfur-Cycling in the Environment
Published in Frontiers in microbiology (26-05-2016)“…Stable isotope probing (SIP) is a valuable tool for gaining insights into ecophysiology and biogeochemical cycling of environmental microbial communities by…”
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