Search Results - "Schellenberger, V."
-
1
Construction of stabilized proteins by combinatorial consensus mutagenesis
Published in Protein engineering, design and selection (01-11-2004)“…We constructed stabilized variants of β-lactamase (BLA) from Enterobacter cloacae by combinatorial recruitment of consensus mutations. By aligning the…”
Get full text
Journal Article -
2
Selection of a subtilisin-hyperproducing Bacillus in a highly structured environment
Published in Applied microbiology and biotechnology (01-03-1998)“…Mutants that secrete increased amounts of enzyme into a selection medium can be efficiently enriched from large populations of mutagenized microorganisms…”
Get full text
Journal Article -
3
-
4
-
5
-
6
The substrate specificity of Uca pugilator collagenolytic serine protease 1 correlates with the bovine type I collagen cleavage sites
Published in The Journal of biological chemistry (29-07-1994)“…Affinity-based purification and characterization of the collagenolytic serine protease 1 from Uca pugilator (fiddler crab) hepatopancreas shows that the enzyme…”
Get full text
Journal Article -
7
Extension of in vivo half-life of biologically active peptides via chemical conjugation to XTEN protein polymer
Published in Protein engineering, design and selection (01-11-2013)“…XTEN, unstructured biodegradable proteins, have been used to extend the in vivo half-life of genetically fused therapeutic proteins and peptides. To expand the…”
Get full text
Journal Article -
8
Rapid Evolution of Novel Traits in Microorganisms
Published in Applied and Environmental Microbiology (01-08-2001)“…Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
Get full text
Journal Article -
9
Generation of large libraries of random mutants in Bacillus subtilis by PCR-based plasmid multimerization
Published in BioTechniques (01-08-1997)“…We describe a PCR-based method for the generation of plasmid multimers that can be directly transformed into Bacillus subtilis with very high efficiency. This…”
Get full text
Journal Article -
10
Screening of synthetic peptide libraries with radiolabeled acceptor molecules
Published in Peptide research (01-05-1993)“…A method has been developed for the identification of specific acceptor molecule-binding sequences from a chemically synthesized peptide library. The peptide…”
Get more information
Journal Article -
11
Protease-catalyzed peptide synthesis: prevention of side reactions in kinetically controlled reactions
Published in Peptide research (01-09-1991)“…One major problem in protease-catalyzed peptide synthesis is the occurrence of unwanted proteolytic side reactions. The objective of this study was to…”
Get more information
Journal Article -
12
Role of the S' Subsites in Serine Protease Catalysis. Active-Site Mapping of Rat Chymotrypsin, Rat Trypsin, .alpha.-Lytic Protease, and Cercarial Protease from Schistosoma mansoni
Published in Biochemistry (Easton) (12-04-1994)“…The S' subsite specificity of four homologous serine proteases, rat chymotrypsin, rat trypsin, alpha-lytic protease, and cercarial protease from Schistosoma…”
Get full text
Journal Article -
13
Mapping the S' subsites of serine proteases using acyl transfer to mixtures of peptide nucleophiles
Published in Biochemistry (Easton) (27-04-1993)“…We have developed a rapid and convenient procedure for the characterization of the S' subsite specificity of serine proteases. A mixture of peptide…”
Get full text
Journal Article -
14
Rapid in vivo evolution of a β-lactamase using phagemids
Published in Biotechnology and bioengineering (05-04-2000)“…RNA viruses are capable of undergoing extremely rapid evolution due to their high rates of reproduction, small genome size, and a high frequency of spontaneous…”
Get full text
Journal Article -
15
Synthesis and kinetic characterisation of omega-guanidinocarbonic acid ethyl esters as trypsin substrates
Published in Biomedica biochimica acta (1990)“…Aliphatic omega-guanidinocarbonic acid ethyl esters of different chain length (C3-C6) were synthesized and characterized as 4-toluenesulfonates. The kinetic…”
Get more information
Journal Article -
16
Chymotrypsin-catalyzed fragment coupling synthesis of GnRH-analogs
Published in Biomedica biochimica acta (1991)“…D-Xaa6-GnRH analogs (Xaa: Ala, Nal1), Phe, Ser(tBu), Trp) were prepared by chymotrypsin catalyzed 3 + 7 fragment coupling synthesis with conversion rates of…”
Get more information
Journal Article -
17
Substrate preferences of Vsr DNA mismatch endonuclease and their consequences for the evolution of the Escherichia coli K-12 genome
Published in Journal of molecular biology (1995)“…The substrate spectrum of Vsr DNA mismatch endonuclease of Escherichia coli K-12 was investigated using fluorescence-labelled oligonucleotide Substrates and a…”
Get full text
Journal Article -
18
Analysis of enzyme specificity by multiple substrate kinetics
Published in Biochemistry (Easton) (27-04-1993)“…Multiple approaches for screening large sets of compounds for a specific function are of growing interest. The use of substrate mixtures to characterize the…”
Get full text
Journal Article -
19
Penicillin acylase-catalyzed protection and deprotection of amino groups as a promising approach in enzymatic peptide synthesis
Published in FEBS letters (05-08-1991)“…Penicillin acylase from E. coli is able to catalyze both the introduction and the removal of the phenylacetyl group. We have established that phenylacetyl…”
Get full text
Journal Article -
20
Electrostatic effects in the alpha-chymotrypsin-catalyzed acyl transfer. II. Efficiency of nucleophiles bearing charged groups in various locations
Published in Biochimica et biophysica acta (30-05-1991)“…We investigated the alpha-chymotrypsin-catalyzed acyl transfer to a series of glycine oligomers. It could be established that the electrostatic interactions…”
Get more information
Journal Article