Search Results - "Schasfoort, Richard B."

Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling by de Lau, Wim, Barker, Nick, Low, Teck Y., Koo, Bon-Kyoung, Li, Vivian S. W., Teunissen, Hans, Kujala, Pekka, Haegebarth, Andrea, Peters, Peter J., van de Wetering, Marc, Stange, D. E., van Es, J., Guardavaccaro, Daniele, Schasfoort, Richard B. M., Mohri, Yasuaki, Nishimori, Katsuhiko, Mohammed, Shabaz, Heck, Albert J. R., Clevers, Hans

“…The adult stem cell marker Lgr5 and its relative Lgr4 are often co-expressed in Wnt-driven proliferative compartments. We find that conditional deletion of…”
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A microwell array platform to print and measure biomolecules produced by single cells by Abali, Fikri, Broekmaat, Joska, Tibbe, Arjan, Schasfoort, Richard B M, Zeune, Leonie, Terstappen, Leon W M M

“…Here we describe a combined method to monitor the secretion of molecules produced by single cells, followed by a method to isolate the individual cells that…”
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Miniaturizing free-flow electrophoresis - a critical review by Kohlheyer, Dietrich, Eijkel, Jan C.T, van den Berg, Albert, Schasfoort, Richard B.M

“…Free-flow electrophoresis (FFE) separation methods have been developed and investigated for around 50 years and have been applied not only to many types of…”
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Nanoparticle Enhancement Cascade for Sensitive Multiplex Measurements of Biomarkers in Complex Fluids with Surface Plasmon Resonance Imaging by Hendriks, Jan, Stojanovic, Ivan, Schasfoort, Richard B. M, Saris, Daniël B. F, Karperien, Marcel

“…There is a large unmet need for reliable biomarker measurement systems for clinical application. Such systems should meet challenging requirements for large…”
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Analysis of cell surface antigens by Surface Plasmon Resonance imaging by Stojanović, Ivan, Schasfoort, Richard B.M., Terstappen, Leon W.M.M.

“…Surface Plasmon Resonance (SPR) is most commonly used to measure bio-molecular interactions. SPR is used significantly less frequent for measuring whole cell…”
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Field-Effect Flow Control for Microfabricated Fluidic Networks by Richard B. M. Schasfoort, Schlautmann, Stefan, Hendrikse, Jan, van den Berg, Albert

“…The magnitude and direction of the electro-osmotic flow (EOF) inside a microfabricated fluid channel can be controlled by a perpendicular electric field of 1.5…”
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Angle-scanning SPR imaging for detection of biomolecular interactions on microarrays by Beusink, J. Bianca, Lokate, Angelique M.C., Besselink, Geert A.J., Pruijn, Ger J.M., Schasfoort, Richard B.M.

“…In this paper we describe the use of a commercial surface plasmon resonance (SPR) imaging instrument for monitoring the binding of biomolecules on user-defined…”
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Microfluidic High-Resolution Free-Flow Isoelectric Focusing by Kohlheyer, Dietrich, Eijkel, Jan C. T, Schlautmann, Stefan, van den Berg, Albert, Schasfoort, Richard B. M

“…A microfluidic free-flow isoelectric focusing glass chip for separation of proteins is described. Free-flow isoelectric focusing is demonstrated with a set of…”
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Bubble-Free Operation of a Microfluidic Free-Flow Electrophoresis Chip with Integrated Pt Electrodes by Kohlheyer, Dietrich, Eijkel, Jan C. T, Schlautmann, Stefan, van den Berg, Albert, Schasfoort, Richard B. M

“…In order to ensure a stable and efficient separation in microfluidic free-flow electrophoresis (FFE) devices, various methods and chips have been presented…”
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Biomolecular Interaction Monitoring of Autoantibodies by Scanning Surface Plasmon Resonance Microarray Imaging by Lokate, Angelique M. C, Beusink, J. Bianca, Besselink, Geert A. J, Pruijn, Ger J. M, Schasfoort, Richard B. M

“…A new commercial surface plasmon resonance (SPR) imaging analysis system with a novel SPR dip angle scanning principle allows the measurement, without the need…”
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Spermine induced reversible collapse of deoxyribonucleic acid-bridged nanoparticle-based assemblies by Göeken, Kristian L., Schasfoort, Richard B. M., Subramaniam, Vinod, Gill, Ron

“…DNA-linked 2D and 3D nano-assemblies find use in a diverse set of applications, ranging from DNA-origami in drug delivery and medical imaging, to DNA-linked…”
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IgG Subclass Determines Suppression Versus Enhancement of Humoral Alloimmunity to Kell RBC Antigens in Mice by Shinde, Paurvi, Howie, Heather L, Stegmann, Tamara C, Hay, Ariel M, Waterman, Hayley R, Szittner, Zoltan, Bentlage, Arthur E H, Kapp, Linda, Lissenberg-Thunnissen, Suzanne N, Dekkers, Gillian, Schasfoort, Richard B M, Ratcliffe, Sarah J, Smolkin, Mark E, Vidarsson, Gestur, van der Schoot, C Ellen, Hudson, Krystalyn E, Zimring, James C

“…It has long been appreciated that immunoglobulins are not just the effector endpoint of humoral immunity, but rather have a complex role in regulating antibody…”
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Electrokinetic Lab-on-a-BioChip for Multi-ligand/Multi-analyte Biosensing by Krishnamoorthy, Ganeshram, Carlen, Edwin T, deBoer, Hans L, van den Berg, Albert, Schasfoort, Richard B. M

“…We present a simple electrokinetic lab-on-a-biochip (EKLB) with four microchannels integrated with a surface plasmon resonance imaging (iSPR) label-free…”
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Gradient method for accurate affinity determinations by Schasfoort, Richard B.M.

“…The value of the affinity constants (kd, ka, and KD) that are determined by label free interaction analysis methods are strongly affected by the ligand density…”
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Mapping of citrullinated fibrinogen B-cell epitopes in rheumatoid arthritis by imaging surface plasmon resonance by van Beers, Joyce J B C, Raijmakers, Reinout, Alexander, Lou-Ella, Stammen-Vogelzangs, Judith, Lokate, Angelique M C, Heck, Albert J R, Schasfoort, Richard B M, Pruijn, Ger J M

“…Rheumatoid arthritis (RA) frequently involves the loss of tolerance to citrullinated antigens, which may play a role in pathogenicity. Citrullinated fibrinogen…”
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Integrated Electrokinetic Sample Focusing and Surface Plasmon Resonance Imaging System for Measuring Biomolecular Interactions by Krishnamoorthy, Ganeshram, Carlen, Edwin T, Kohlheyer, Dietrich, Schasfoort, Richard B. M, van den Berg, Albert

“…Label-free biomolecular binding measurement methods, such as surface plasmon resonance (SPR), are becoming increasingly more important for the estimation of…”
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Trends in SPR Cytometry: Advances in Label-Free Detection of Cell Parameters by Schasfoort, Richard B M, Abali, Fikri, Stojanovic, Ivan, Vidarsson, Gestur, Terstappen, Leon W M M

“…SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free…”
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Platinum(II) as Bifunctional Linker in Antibody-Drug Conjugate Formation: Coupling of a 4-Nitrobenzo-2-oxa-1,3-diazole Fluorophore to Trastuzumab as a Model by Waalboer, Dennis C. J., Muns, Joey A., Sijbrandi, Niels J., Schasfoort, Richard B. M., Haselberg, Rob, Somsen, Govert W., Houthoff, Hendrik-Jan, van Dongen, Guus A. M. S.

“…The potential of platinum(II) as a bifunctional linker in the coordination of small molecules, such as imaging agents or (cytotoxic) drugs, to monoclonal…”
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Multiplex Label Free Characterization of Cancer Cell Lines Using Surface Plasmon Resonance Imaging by Stojanović, Ivan, Ruivo, Carolina F, van der Velden, Thomas J G, Schasfoort, Richard B M, Terstappen, Leon W M M

“…Rapid multiplex cell surface marker analysis can expedite investigations in which large number of antigens need to be analyzed. Simultaneous analysis of…”
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Electrokinetic label-free screening chip: a marriage of multiplexing and high throughput analysis using surface plasmon resonance imaging by Krishnamoorthy, Ganeshram, Carlen, Edwin T, Bomer, Johan G, Wijnperlé, Daniël, deBoer, Hans L, van den Berg, Albert, Schasfoort, Richard B M

“…We present an electrokinetic label-free biomolecular screening chip (Glass/PDMS) to screen up to 10 samples simultaneously using surface plasmon resonance…”
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