Search Results - "Schaffrath, Raffael"

Induction of protein aggregation and starvation response by tRNA modification defects by Klassen, Roland, Bruch, Alexander, Schaffrath, Raffael

“…Posttranscriptional modifications of anticodon loops contribute to the decoding efficiency of tRNAs by supporting codon recognition and loop stability…”
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Misactivation of multiple starvation responses in yeast by loss of tRNA modifications by Bruch, Alexander, Laguna, Teresa, Butter, Falk, Schaffrath, Raffael, Klassen, Roland

“…Abstract Previously, combined loss of different anticodon loop modifications was shown to impair the function of distinct tRNAs in Saccharomyces cerevisiae…”
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Role of SSD1 in Phenotypic Variation of Saccharomyces cerevisiae Strains Lacking DEG1-Dependent Pseudouridylation by Khonsari, Bahar, Klassen, Roland, Schaffrath, Raffael

“…Yeast phenotypes associated with the lack of wobble uridine (U34) modifications in tRNA were shown to be modulated by an allelic variation of SSD1, a gene…”
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Evolutionary Conservation in Protein-Protein Interactions and Structures of the Elongator Sub-Complex ELP456 from Arabidopsis and Yeast by Jun, Sang Eun, Cho, Kiu-Hyung, Schaffrath, Raffael, Kim, Gyung-Tae

“…The Elongator complex plays a pivotal role in the wobble uridine modification of the tRNA anticodon. Comprising two sets of six distinct subunits, namely,…”
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DPH1 and DPH2 variants that confer susceptibility to diphthamide deficiency syndrome in human cells and yeast models by Ütkür, Koray, Mayer, Klaus, Khan, Maliha, Manivannan, Thirishika, Schaffrath, Raffael, Brinkmann, Ulrich

“…ABSTRACT The autosomal-recessive diphthamide deficiency syndrome presents as intellectual disability with developmental abnormalities, seizures, craniofacial…”
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Diphthamide-deficiency syndrome: a novel human developmental disorder and ribosomopathy by Hawer, Harmen, Mendelsohn, Bryce A, Mayer, Klaus, Kung, Ann, Malhotra, Amit, Tuupanen, Sari, Schleit, Jennifer, Brinkmann, Ulrich, Schaffrath, Raffael

“…We describe a novel type of ribosomopathy that is defined by deficiency in diphthamidylation of translation elongation factor 2. The ribosomopathy was…”
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Translational fidelity and growth of Arabidopsis require stress-sensitive diphthamide biosynthesis by Zhang, Hongliang, Quintana, Julia, Ütkür, Koray, Adrian, Lorenz, Hawer, Harmen, Mayer, Klaus, Gong, Xiaodi, Castanedo, Leonardo, Schulten, Anna, Janina, Nadežda, Peters, Marcus, Wirtz, Markus, Brinkmann, Ulrich, Schaffrath, Raffael, Krämer, Ute

“…Diphthamide, a post-translationally modified histidine residue of eukaryotic TRANSLATION ELONGATION FACTOR2 (eEF2), is the human host cell-sensitizing target…”
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The diphthamide modification pathway from Saccharomyces cerevisiae – revisited by Schaffrath, Raffael, Abdel‐Fattah, Wael, Klassen, Roland, Stark, Michael J. R.

“…Summary Diphthamide is a conserved modification in archaeal and eukaryal translation elongation factor 2 (EF2). Its name refers to the target function for…”
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Roles of Elongator Dependent tRNA Modification Pathways in Neurodegeneration and Cancer by Hawer, Harmen, Hammermeister, Alexander, Ravichandran, Keerthiraju Ethiraju, Glatt, Sebastian, Schaffrath, Raffael, Klassen, Roland

“…Transfer RNA (tRNA) is subject to a multitude of posttranscriptional modifications which can profoundly impact its functionality as the essential adaptor…”
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Importance of diphthamide modified EF2 for translational accuracy and competitive cell growth in yeast by Hawer, Harmen, Ütkür, Koray, Arend, Meike, Mayer, Klaus, Adrian, Lorenz, Brinkmann, Ulrich, Schaffrath, Raffael

“…In eukaryotes, the modification of an invariant histidine (His-699 in yeast) residue in translation elongation factor 2 (EF2) with diphthamide involves a…”
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Absolute Quantification of Noncoding RNA by Microscale Thermophoresis by Jacob, Dominik, Thüring, Kathrin, Galliot, Aurellia, Marchand, Virginie, Galvanin, Adeline, Ciftci, Akif, Scharmann, Karin, Stock, Michael, Roignant, Jean‐Yves, Leidel, Sebastian A., Motorin, Yuri, Schaffrath, Raffael, Klassen, Roland, Helm, Mark

“…Accurate quantification of the copy numbers of noncoding RNA has recently emerged as an urgent problem, with impact on fields such as RNA modification…”
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Loss of anticodon wobble uridine modifications affects tRNA(Lys) function and protein levels in Saccharomyces cerevisiae by Klassen, Roland, Grunewald, Pia, Thüring, Kathrin L, Eichler, Christian, Helm, Mark, Schaffrath, Raffael

“…In eukaryotes, wobble uridines in the anticodons of tRNA(Lys)UUU, tRNA(Glu)UUC and tRNA(Gln)UUG are modified to 5-methoxy-carbonyl-methyl-2-thio-uridine…”
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Cryo-EM structures of the human Elongator complex at work by Abbassi, Nour-el-Hana, Jaciuk, Marcin, Scherf, David, Böhnert, Pauline, Rau, Alexander, Hammermeister, Alexander, Rawski, Michał, Indyka, Paulina, Wazny, Grzegorz, Chramiec-Głąbik, Andrzej, Dobosz, Dominika, Skupien-Rabian, Bozena, Jankowska, Urszula, Rappsilber, Juri, Schaffrath, Raffael, Lin, Ting-Yu, Glatt, Sebastian

“…tRNA modifications affect ribosomal elongation speed and co-translational folding dynamics. The Elongator complex is responsible for introducing…”
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Eukaryotic life without tQCUG: the role of Elongator-dependent tRNA modifications in Dictyostelium discoideum by Schäck, Manfred A, Jablonski, Kim Philipp, Gräf, Stefan, Klassen, Roland, Schaffrath, Raffael, Kellner, Stefanie, Hammann, Christian

“…Abstract In the Elongator-dependent modification pathway, chemical modifications are introduced at the wobble uridines at position 34 in transfer RNAs (tRNAs),…”
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DPH1 Gene Mutations Identify a Candidate SAM Pocket in Radical Enzyme Dph1•Dph2 for Diphthamide Synthesis on EF2 by Ütkür, Koray, Schmidt, Sarina, Mayer, Klaus, Klassen, Roland, Brinkmann, Ulrich, Schaffrath, Raffael

“…In eukaryotes, the Dph1•Dph2 dimer is a non-canonical radical SAM enzyme. Using iron-sulfur (FeS) clusters, it cleaves the cosubstrate S-adenosyl-methionine…”
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Functional Integrity of Radical SAM Enzyme Dph1•Dph2 Requires Non-Canonical Cofactor Motifs with Tandem Cysteines by Ütkür, Koray, Mayer, Klaus, Liu, Shihui, Brinkmann, Ulrich, Schaffrath, Raffael

“…The Dph1•Dph2 heterodimer from yeast is a radical SAM (RS) enzyme that generates the 3-amino-3-carboxy-propyl (ACP) precursor for diphthamide, a clinically…”
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Role of Pseudouridine Formation by Deg1 for Functionality of Two Glutamine Isoacceptor tRNAs by Klassen, Roland, Schaffrath, Raffael

“…Loss of Deg1/Pus3 and concomitant elimination of pseudouridine in tRNA at positions 38 and 39 (ψ38/39) was shown to specifically impair the function of tRNA…”
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Autoselection of cytoplasmic yeast virus like elements encoding toxin/antitoxin systems involves a nuclear barrier for immunity gene expression by Kast, Alene, Voges, Raphael, Schroth, Michael, Schaffrath, Raffael, Klassen, Roland, Meinhardt, Friedhelm

“…Cytoplasmic virus like elements (VLEs) from Kluyveromyces lactis (Kl), Pichia acaciae (Pa) and Debaryomyces robertsiae (Dr) are extremely A/T-rich (>75%) and…”
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AtELP4 a subunit of the Elongator complex in Arabidopsis, mediates cell proliferation and dorsoventral polarity during leaf morphogenesis by Jun, Sang Eun, Cho, Kiu-Hyung, Manzoor, Muhammad Aamir, Hwang, Tae Young, Kim, Youn Soo, Schaffrath, Raffael, Kim, Gyung-Tae

“…The Elongator complex in eukaryotes has conserved tRNA modification functions and contributes to various physiological processes such as transcriptional…”
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Redox requirements for ubiquitin-like urmylation of Ahp1, a 2-Cys peroxiredoxin from yeast by Brachmann, Cindy, Kaduhr, Lars, Jüdes, André, Ravichandran, Keerthiraju Ethiraju, West, James D., Glatt, Sebastian, Schaffrath, Raffael

“…The yeast peroxiredoxin Ahp1, like related anti-oxidant enzymes in other species, undergoes urmylation, a lysine-directed conjugation to ubiquitin-like…”
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