Search Results - "Schaffer, Miroslava"

Sample preparation for atomic-resolution STEM at low voltages by FIB by Schaffer, Miroslava, Schaffer, Bernhard, Ramasse, Quentin

“…While FIB sample preparation for transmission electron microscopy is a well established technique, few examples exist of samples of sufficient quality for…”
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Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography by Engel, Benjamin D, Schaffer, Miroslava, Kuhn Cuellar, Luis, Villa, Elizabeth, Plitzko, Jürgen M, Baumeister, Wolfgang

“…Chloroplast function is orchestrated by the organelle's intricate architecture. By combining cryo-focused ion beam milling of vitreous Chlamydomonas cells with…”
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Visualizing the molecular sociology at the HeLa cell nuclear periphery by Mahamid, Julia, Pfeffer, Stefan, Schaffer, Miroslava, Villa, Elizabeth, Danev, Radostin, Cuellar, Luis Kuhn, Förster, Friedrich, Hyman, Anthony A., Plitzko, Jürgen M., Baumeister, Wolfgang

“…The molecular organization of eukaryotic nuclear volumes remains largely unexplored. Here we combined recent developments in cryo–electron tomography (cryo-ET)…”
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Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography by Wagner, Felix R., Watanabe, Reika, Schampers, Ruud, Singh, Digvijay, Persoon, Hans, Schaffer, Miroslava, Fruhstorfer, Peter, Plitzko, Jürgen, Villa, Elizabeth

“…Recent advances have made cryogenic (cryo) electron microscopy a key technique to achieve near-atomic-resolution structures of biochemically isolated…”
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Charting the native architecture of Chlamydomonas thylakoid membranes with single-molecule precision by Wietrzynski, Wojciech, Schaffer, Miroslava, Tegunov, Dimitry, Albert, Sahradha, Kanazawa, Atsuko, Plitzko, Jürgen M, Baumeister, Wolfgang, Engel, Benjamin D

“…Thylakoid membranes scaffold an assortment of large protein complexes that work together to harness the energy of light. It has been a longstanding challenge…”
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Optimized cryo-focused ion beam sample preparation aimed at in situ structural studies of membrane proteins by Schaffer, Miroslava, Mahamid, Julia, Engel, Benjamin D., Laugks, Tim, Baumeister, Wolfgang, Plitzko, Jürgen M.

“…While cryo-electron tomography (cryo-ET) can reveal biological structures in their native state within the cellular environment, it requires the production of…”
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The structure of the COPI coat determined within the cell by Bykov, Yury S, Schaffer, Miroslava, Dodonova, Svetlana O, Albert, Sahradha, Plitzko, Jürgen M, Baumeister, Wolfgang, Engel, Benjamin D, Briggs, John Ag

“…COPI-coated vesicles mediate trafficking within the Golgi apparatus and from the Golgi to the endoplasmic reticulum. The structures of membrane protein coats,…”
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The Eukaryotic CO2-Concentrating Organelle Is Liquid-like and Exhibits Dynamic Reorganization by Freeman Rosenzweig, Elizabeth S., Xu, Bin, Kuhn Cuellar, Luis, Martinez-Sanchez, Antonio, Schaffer, Miroslava, Strauss, Mike, Cartwright, Heather N., Ronceray, Pierre, Plitzko, Jürgen M., Förster, Friedrich, Wingreen, Ned S., Engel, Benjamin D., Mackinder, Luke C.M., Jonikas, Martin C.

“…Approximately 30%–40% of global CO2 fixation occurs inside a non-membrane-bound organelle called the pyrenoid, which is found within the chloroplasts of most…”
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Structure of the membrane-assembled retromer coat determined by cryo-electron tomography by Kovtun, Oleksiy, Leneva, Natalya, Bykov, Yury S., Ariotti, Nicholas, Teasdale, Rohan D., Schaffer, Miroslava, Engel, Benjamin D., Owen, David. J., Briggs, John A. G., Collins, Brett M.

“…Eukaryotic cells traffic proteins and lipids between different compartments using protein-coated vesicles and tubules. The retromer complex is required to…”
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In situ architecture of the ciliary base reveals the stepwise assembly of intraflagellar transport trains by van den Hoek, Hugo, Klena, Nikolai, Jordan, Mareike A., Alvarez Viar, Gonzalo, Righetto, Ricardo D., Schaffer, Miroslava, Erdmann, Philipp S., Wan, William, Geimer, Stefan, Plitzko, Jürgen M., Baumeister, Wolfgang, Pigino, Gaia, Hamel, Virginie, Guichard, Paul, Engel, Benjamin D.

“…The cilium is an antenna-like organelle that performs numerous cellular functions, including motility, sensing, and signaling. The base of the cilium contains…”
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Structural basis for VIPP1 oligomerization and maintenance of thylakoid membrane integrity by Gupta, Tilak Kumar, Klumpe, Sven, Gries, Karin, Heinz, Steffen, Wietrzynski, Wojciech, Ohnishi, Norikazu, Niemeyer, Justus, Spaniol, Benjamin, Schaffer, Miroslava, Rast, Anna, Ostermeier, Matthias, Strauss, Mike, Plitzko, Jürgen M., Baumeister, Wolfgang, Rudack, Till, Sakamoto, Wataru, Nickelsen, Jörg, Schuller, Jan M., Schroda, Michael, Engel, Benjamin D.

“…Vesicle-inducing protein in plastids 1 (VIPP1) is essential for the biogenesis and maintenance of thylakoid membranes, which transform light into life…”
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In situ architecture of the algal nuclear pore complex by Mosalaganti, Shyamal, Kosinski, Jan, Albert, Sahradha, Schaffer, Miroslava, Strenkert, Daniela, Salomé, Patrice A., Merchant, Sabeeha S., Plitzko, Jürgen M., Baumeister, Wolfgang, Engel, Benjamin D., Beck, Martin

“…Nuclear pore complexes (NPCs) span the nuclear envelope and mediate nucleocytoplasmic exchange. They are a hallmark of eukaryotes and deeply rooted in the…”
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Determining the bacterial cell biology of Planctomycetes by Boedeker, Christian, Schüler, Margarete, Reintjes, Greta, Jeske, Olga, van Teeseling, Muriel C. F., Jogler, Mareike, Rast, Patrick, Borchert, Daniela, Devos, Damien P., Kucklick, Martin, Schaffer, Miroslava, Kolter, Roberto, van Niftrik, Laura, Engelmann, Susanne, Amann, Rudolf, Rohde, Manfred, Engelhardt, Harald, Jogler, Christian

“…Bacteria of the phylum Planctomycetes have been previously reported to possess several features that are typical of eukaryotes, such as cytosolic…”
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Direct visualization of degradation microcompartments at the ER membrane by Albert, Sahradha, Wietrzynski, Wojciech, Lee, Chia-Wei, Schaffer, Miroslava, Beck, Florian, Schuller, Jan M., Salomé, Patrice A., Plitzko, Jürgen M., Baumeister, Wolfgang, Engel, Benjamin D.

“…To promote the biochemical reactions of life, cells can compartmentalize molecular interaction partners together within separated non–membrane-bound regions…”
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Dissecting the molecular organization of the translocon-associated protein complex by Pfeffer, Stefan, Dudek, Johanna, Schaffer, Miroslava, Ng, Bobby G., Albert, Sahradha, Plitzko, Jürgen M., Baumeister, Wolfgang, Zimmermann, Richard, Freeze, Hudson H., Engel, Benjamin D., Förster, Friedrich

“…In eukaryotic cells, one-third of all proteins must be transported across or inserted into the endoplasmic reticulum (ER) membrane by the ER protein…”
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In situ structural analysis of Golgi intracisternal protein arrays by Engel, Benjamin D., Schaffer, Miroslava, Albert, Sahradha, Asano, Shoh, Plitzko, Jürgen M., Baumeister, Wolfgang

“…We acquired molecular-resolution structures of the Golgi within its native cellular environment. VitreousChlamydomonascells were thinned by cryo-focused ion…”
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Molecular and structural architecture of polyQ aggregates in yeast by Gruber, Anselm, Hornburg, Daniel, Antonin, Matthias, Krahmer, Natalie, Collado, Javier, Schaffer, Miroslava, Zubaite, Greta, Lüchtenborg, Christian, Sachsenheimer, Timo, Brügger, Britta, Mann, Matthias, Baumeister, Wolfgang, Hartl, F. Ulrich, Hipp, Mark S., Fernández-Busnadiego, Rubén

“…Huntington’s disease is caused by the expansion of a polyglutamine (polyQ) tract in the N-terminal exon of huntingtin (HttEx1), but the cellular mechanisms…”
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Architecture of the centriole cartwheel‐containing region revealed by cryo‐electron tomography by Klena, Nikolai, Le Guennec, Maeva, Tassin, Anne‐Marie, van den Hoek, Hugo, Erdmann, Philipp S, Schaffer, Miroslava, Geimer, Stefan, Aeschlimann, Gabriel, Kovacik, Lubomir, Sadian, Yashar, Goldie, Kenneth N, Stahlberg, Henning, Engel, Benjamin D, Hamel, Virginie, Guichard, Paul

“…Centrioles are evolutionarily conserved barrels of microtubule triplets that form the core of the centrosome and the base of the cilium. While the crucial role…”
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Pleomorphic linkers as ubiquitous structural organizers of vesicles in axons by Schrod, Nikolas, Vanhecke, Dimitri, Laugks, Ulrike, Stein, Valentin, Fukuda, Yoshiyuki, Schaffer, Miroslava, Baumeister, Wolfgang, Lucic, Vladan

“…Many cellular processes depend on a precise structural organization of molecular components. Here, we established that neurons grown in culture provide a…”
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A helical inner scaffold provides a structural basis for centriole cohesion by Le Guennec, Maeva, Klena, Nikolai, Gambarotto, Davide, Laporte, Marine H, Tassin, Anne-Marie, van den Hoek, Hugo, Erdmann, Philipp S, Schaffer, Miroslava, Kovacik, Lubomir, Borgers, Susanne, Goldie, Kenneth N, Stahlberg, Henning, Bornens, Michel, Azimzadeh, Juliette, Engel, Benjamin D, Hamel, Virginie, Guichard, Paul

“…The ninefold radial arrangement of microtubule triplets (MTTs) is the hallmark of the centriole, a conserved organelle crucial for the formation of centrosomes…”
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