Search Results - "Schaefer, F.W."

Statistical assessment of DNA extraction reagent lot variability in real-time quantitative PCR by Bushon, R.N, Kephart, C.M, Koltun, G.F, Francy, D.S, Schaefer, F.W. III, Alan Lindquist, H.D

“…The aim of this study was to evaluate the variability in lots of a DNA extraction kit using real-time PCR assays for Bacillus anthracis, Francisella tularensis…”
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Species-specific detection of three human-pathogenic microsporidial species from the genus Encephalitozoon via fluorogenic 5′ nuclease PCR assays by Hester, J.D., Varma, M., Bobst, A.M., Ware, M.W., Lindquist, H.D.A., Schaefer, F.W.

“…This study describes fluorogenic 5′ nuclease PCR assays suitable for rapid, sensitive, high-throughput detection of the human-pathogenic microsporidial species…”
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Comparison of traditional and molecular analytical methods for detecting biological agents in raw and drinking water following ultrafiltration by Francy, D.S, Bushon, R.N, Brady, A.M.G, Bertke, E.E, Kephart, C.M, Likirdopulos, C.A, Mailot, B.E, Schaefer III, F.W, Alan Lindquist, H.D

“…To compare the performance of traditional methods to quantitative polymerase chain reaction (qPCR) for detecting five biological agents in large-volume…”
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Molecular characterization of microsporidia indicates that wild mammals harbor host-adapted Enterocytozoon spp. as well as human-pathogenic Enterocytozoon bieneusi by Sulaiman, I.M, Fayer, R, Lal, A.A, Trout, J.M, Schaefer, F.W. III, Xiao, L

“…Over 13 months, 465 beavers, foxes, muskrats, otters, and raccoons were trapped in four counties in eastern Maryland and examined by molecular methods for…”
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Detection of multiple waterborne pathogens using microsequencing arrays by Brinkman, N.E., Francisco, R., Nichols, T.L., Robinson, D., Schaefer, F.W., Schaudies, R.P., Villegas, E.N.

“…Aims A microarray was developed to simultaneously detect Cryptosporidium parvum, Cryptosporidium hominis, Enterococcus faecium, Bacillus anthracis and…”
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Changes in mouse circulating leukocyte numbers in C57BL/6 mice immunosuppressed with dexamethasone for Cryptosporidium parvum oocyst production by Miller, Thomas A., Schaefer, Frank W.

“…The Iowa strain of Cryptosporidium parvum will not propagate in immunocompetent mice, but will successfully infect genetically immunocompromised nude or SCID…”
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Chemically and genetically immunocompromised mice are not more susceptible than immunocompetent mice to infection with Cryptosporidium muris by Miller, T.A, Ware, M.W, Wymer, L.J, Schaefer, F.W. III

“…The prevailing paradigm is that immunosuppressed individuals are more susceptible to infection and are at higher risk of infection from Cryptosporidium oocysts…”
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Characterization of a single dose methylprednisolone acetate immune suppression model using Cryptosporidium muris and Cryptosporidium parvum by Miller, Thomas A., Schaefer, Frank W.

“…An immunosuppressive dose of methylprednisolone acetate (MPA) was compared with a non-immunosuppressive dose using Cryptosporidium oocyst production as an…”
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Characterization of a Cryptosporidium muris infection and reinfection in CF-1 mice by Miller, Thomas A., Schaefer, Frank W.

“…To establish control values for circulating cells and immune associated organs over the course of a self-limiting Cryptosporidium muris infection and…”
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Methylprednisolone acetate immune suppression produces differing effects on Cryptosporidium muris oocyst production depending on when administered by Miller, Thomas A., Schaefer, Frank W.

“…At different times after inoculation with Cryptosporidium muris, infected CF-1 female mice were immunosuppressed with a single subcutaneous dose of…”
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The effects of time and temperature on flow cytometry enumerated live Cryptosporidium parvum oocysts by Ware, M.W., Schaefer, F.W.

“…Aims:  Recoveries of spiked standard suspensions are used to evaluate method performance. For many applications, γ‐irradiated Cryptosporidium oocysts are…”
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Low pressure ultraviolet studies for inactivation of Giardia muris cysts by Hayes, S.L., Rice, E.W., Ware, M.W., Schaefer, F.W.

“…Aims: The research was initiated to confirm earlier ultraviolet (u.v.) light inactivation studies performed on Giardia cysts using excystation as the viability…”
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Detection of Giardia in environmental waters by immuno-PCR amplification methods by MAHBUBANI, M. H, SCHAEFER, F. W, JONES, D. D, BEJ, A. K

“…Genomic DNA was extracted either directly from Giardia muris cysts seeded into environmental surface waters or from cysts isolated by immunomagnetic beads…”
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Reliability: of surrogates for determining Cryptosporidium removal by Li, Sylvana Y., Goodrich, James A., Owens, James H., Willeke, Gene E., Schaefer, Frank W., Clark, Robert M.

“…Testing of field-scale bag filtration systems yielded results indicating that 4-6 μm polystyrene microspheres can be used as a reliable surrogate for…”
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Comparison of animal infectivity, excystation, and fluorogenic dye as measures of Giardia muris cyst inactivation by ozone by Labatiuk, C W, Schaefer, 3rd, F W, Finch, G R, Belosevic, M

“…Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
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Improved in vitro excystation procedure for Giardia lamblia cysts by Rice, E.W, Schaefer, F.W. III

“…Giardia lamblia cysts obtained from human symptomatic and asymptomatic donors, improved in vitro excystation procedure…”
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Experimental infection of mongrel dogs with Giardia lamblia cysts and cultured trophozoites by Hewlett, E.L, Andrews, J.S., jr, Ruffier, J, Schaefer, F.W. III

“…Giardia lamblia, dogs, experimental infection with cysts and trophozoites, latency periods and course of cyst excretion were similar to those observed in…”
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Comparison of animal infectivity and excystation as measures of Giardia muris cyst inactivation by chlorine by Hoff, J.C, Rice, E.W, Schaefer, F.W. III

“…Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
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Inactivation of Giardia cysts by chlorine by Rice, E.W, Hoff, J.C, Schaefer, F.W. III

“…Giardia lamblia cysts from symptomatic and asymptomatic human carriers, Giardia muris cysts, inactivation by chlorine…”
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Giardia muris: ultrastructural analysis of in vitro excystation by Coggins, J R, Schaefer, 3rd, F W

“…Giardia muris cysts were examined by transmission electron microscopy before treatment, after induction, and at timed intervals during the incubation phase of…”
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