Search Results - "Schaad, N W"

Cultivation of 'Candidatus Liberibacter asiaticus', 'Ca. L. africanus', and 'Ca. L. americanus' Associated with Huanglongbing by Sechler, A, Schuenzel, E.L, Cooke, P, Donnua, S, Thaveechai, N, Postnikova, E, Stone, A.L, Schneider, W.L, Damsteegt, V.D, Schaad, N.W

“…A new medium designated Liber A has been designed and used to successfully cultivate all three 'Candidatus Liberibacter spp.,' the suspect causative agents of…”
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Real-time polymerase chain reaction for one-hour on-site diagnosis of Pierce's disease of grape in early season asymptomatic vines by SCHAAD, N. W, OPGENORTH, D, GAUSH, P

“…ABSTRACT Molecular-based techniques, such as polymerase chain reaction (PCR), can reduce the time needed for diagnosis of plant diseases when compared with…”
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high throughput membrane BIO-PCR technique for ultra-sensitive detection of Pseudomonas syringae pv. phaseolicola by Schaad, N.W, Berthier-Schaad, Y, Knorr, D

“…Molecular-based methods such as PCR have greatly improved detection of bacteria in environmental samples. However, the sensitivity of PCR is not high when…”
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Survival of Clavibacter michiganensis ssp. michiganensis in infected tomato stems under natural field conditions in California, Ohio and Morocco by Fatmi, M., Schaad, N. W.

“…The survival and half‐life of Clavibacter michiganensis ssp. michiganensis (C. michiganensis), the causal agent of bacterial canker of tomato, were determined…”
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Genetic Diversity in Populations of Xanthomonas campestris pv. campestris in Cruciferous Weeds in Central Coastal California by Ignatov, A, Sechler, A, Schuenzel, E.L, Agarkova, I, Oliver, B, Vidaver, A.K, Schaad, N.W

“…Xanthomonas campestris pv. campestris (X. campestris) infects a large number of cruciferous plants, including weeds. California has one of the largest and most…”
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Detection of Acidovorax avenae ssp. avenae in rice seeds using BIO-PCR by Song, W.Y, Kim, H.M, Hwang, C.Y, Schaad, N.W

“…The bacterium, Acidovorax avenae ssp. avenae causes several important plant diseases including bacterial stripe of rice, bacterial stalk rot of corn, bacterial…”
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Identification of Bacterial Plant Pathogens Using Multilocus Polymerase Chain Reaction/Electrospray Ionization-Mass Spectrometry by Postnikova, E, Baldwin, C, Whitehouse, C.A, Sechler, A, Schaad, N.W, Sampath, R, Harpin, V, Li, F, Melton, R, Blyn, L, Drader, J, Hofstadler, S, Schneider, W.L

“…Polymerase chain reaction/electrospray ionization-mass spectrometry (PCR/ESI-MS, previously known as “TIGER”) utilizes PCR with broad-range primers to amplify…”
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Genetic Characterization and Diversity of Rathayibacter toxicus by Agarkova, I.V, Vidaver, A.K, Postnikova, E.N, Riley, I.T, Schaad, N.W

“…Rathayibacter toxicus is a nematode-vectored gram-positive bacterium responsible for a gumming disease of grasses and production of a highly potent animal and…”
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combined agar-absorption and BIO-PCR assay for rapid, sensitive detection of Xylella fastidiosa in grape and citrus by Fatmi, M, Damsteegt, V.D, Schaad, N.W

“…Application of the polymerase chain reaction (PCR) to disease diagnosis is limited in part by the presence of PCR inhibitors. Inhibition can be overcome and…”
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Identification and differentiation of Tilletia indica and T. walkeri using the polymerase chain reaction by FREDERICK, R. D, SNYDER, K. E, TOOLEY, P. W, BERTHIER-SCHAAD, Y, PETERSON, G. L, BONDE, M. R, SCHAAD, N. W, KNORR, D. A

“…ABSTRACT Karnal bunt of wheat, caused by Tilletia indica, was found in regions of the southwestern United States in 1996. Yield losses due to Karnal bunt are…”
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Evaluation of proposed amended names of several pseudomonads and xanthomonads and recommendations by SCHAAD, N. W, VIDAVER, A. K, LACY, G. H, RUDOLPH, K, JONES, J. B

“…ABSTRACT In 1980, over 90% of all plant-pathogenic pseudomonads and xanthomonads were lumped into Pseudomonas syringae and Xanthomonas campestris,…”
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assessment model for rating high-threat crop pathogens by Schaad, N.W, Abrams, J, Madden, L.V, Frederick, R.D, Luster, D.G, Damsteegt, V.D, Vidaver, A.K

“…Natural, accidental, and deliberate introductions of nonindigenous crop pathogens have become increasingly recognized as threats to the U.S. economy. Given the…”
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A combined biological and enzymatic amplification (BIO-PCR) technique to detect Pseudomonas syringae pv. phaseolicola in bean seed extracts by Schaad, N.W. (USDA, ARS, Foreign Disease-Weed Science Research, Ft. Detrick, Frederick, MD.), Cheong, S.S, Tamaki, S, Hatziloukas, E, Panopoulos, N.J

“…A polymerase chain reaction (PCR) method, hereafter referred to as BIO-PCR, that combines biological and enzymatic amplification of PCR targets and simplified…”
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Reclassification of the Xanthomonads Associated with Bacterial Spot Disease of Tomato and Pepper by Jones, Jeffrey B., Lacy, George H., Bouzar, Hacene, Stall, Robert E., Schaad, Norman W.

“…Four phenotypic xanthomonad groups have been identified that are pathogenic to pepper, tomato, or both hosts. These include groups A and C which are found in…”
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Comparison of PCR, BIO‐PCR, DIA, ELISA and isolation on semiselective medium for detection of Xanthomonas albilineans, the causal agent of leaf scald of sugarcane by WANG, Z. K, COMSTOCK, J. C, HATZILOUKAS, E, SCHAAD, N. W

“…Polymerase chain reaction (PCR) and newly designed primers, XAF1/XAR1, were tested for selective detection of the causal agent of leaf scald of sugarcane,…”
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Sensitive molecular diagnostic assays to mitigate the risks of asymptomatic bacterial diseases of plants by Schaad, N, Schuenzel, E

“…Our highly concentrated monoculture makes crops vulnerable to pests and diseases. An increase in emerging non-indigenous bacterial diseases poses a real threat…”
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Xylella fastidiosa subspecies: X. fastidiosa subsp piercei, subsp. nov., X. fastidiosa subsp. multiplex subsp. nov., and X. fastidiosa subsp. pauca subsp. nov by Schaad, Norman W., Postnikova, Elena, Lacy, George, Fatmi, M'Barek, Chang, Chung-Jan

“…Xylella fastidiosa, a fastidious bacterium causing disease in over 100 plant species, is classified as a single species, although genetic studies support…”
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Isolation of a species-specific mitochondrial DNA sequence for identification of Tilletia indica, the karnal bunt of wheat fungus by Ferreira, M.A.S.V. (Universidade de Brasilia, Brasilia, Brazil.), Tooley, P.W, Hatziloukas, E, Castro, C, Schaad, N.W

“…Mitochondrial DNA (mtDNA) from five isolates of Tilletia indica was isolated and digested with several restriction enzymes. A 2.3-kb EcoRI fragment was chosen,…”
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Advances in molecular-based diagnostics in meeting crop biosecurity and phytosanitary issues by SCHAAD, Norman W, FREDERICK, Reid D, SHAW, Joe, SCHNEIDER, William L, HICKSON, Robert, PETRILLO, Michael D, LUSTER, Douglas G

“…Awareness of crop biosecurity and phytosanitation has been heightened since 9/11 and the unresolved anthrax releases in October 2001. Crops are highly…”
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Specific detection of Pseudomonas syringae pv. phaseolicola DNA in bean seed by polymerase chain reaction-based amplification of a phaseolotoxin gene region by Prosen, D, Hatziloukas, E, Schaad, N.W, Panopoulos, N.J

“…The polymerase chain reaction (PCR) is described for the specific detection of the bean halo blight pathogen Pseudomonas syringae pv. phaseolicola in bean…”
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