Search Results - "Scawen, Michael D."

A study of the interactions between an IgG-binding domain based on the B domain of staphylococcal Protein A and rabbit IgG by BROWN, N. L, BOTTOMLEY, S. P, SCAWEN, M. D, GORE, M. G

“…The nonantigenic interaction between a recombinant immunoglobulin G (IgG)-binding protein based on the B domain of Protein A from Staphylococcus aureus (termed…”
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Tetrameric malate dehydrogenase from a thermophilic Bacillus: cloning, sequence and overexpression of the gene encoding the enzyme and isolation and characterization of the recombinant enzyme by Wynne, S A, Nicholls, D J, Scawen, M D, Sundaram, T K

“…The gene encoding the tetrameric malate dehydrogenase (MDH) in a thermophilic Bacillus species (BI) has been cloned in an Escherichia coli plasmid. The…”
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The importance of arginine 102 for the substrate specificity of Escherichia coli malate dehydrogenase by Nicholls, D J, Miller, J, Scawen, M D, Clarke, A R, Holbrook, J J, Atkinson, T, Goward, C R

“…The malate dehydrogenase from Escherichia coli has been specifically altered at a single amino acid residue by using site-directed mutagenesis. The conserved…”
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Cadmium-Resistant Pseudomonas putida Synthesizes Novel Cadmium Proteins by Higham, Denise P., Sadler, Peter J., Scawen, Michael D.

“…Three cysteine-rich proteins of molecular weight 4000 to 7000, containing 4 to 7 gram atoms of cadmium, zinc, and copper per mole were isolated from…”
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Effects of gold(I) antiarthritic drugs and related compounds on Pseudomonas putida by Rhodes, M D, Sadler, P J, Scawen, M D, Silver, S

“…The effects of the antiarthritic drugs aurothiomalate (AuTm), aurothioglucose (AuTg), auranofin, its metabolite triethylphosphinegold(I)thioglucose (Et3PAuTg),…”
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Cadmium-Binding Proteins in Pseudomonas putida: Pseudothioneins by Higham, Denise P., Sadler, Peter J., Scawen, Michael D.

“…Pseudomonas putida adapted to growth in 3 mM cadmium. The resistance mechanism involved complexation of cadmium in polyphosphate granules, changes in the…”
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Cloning and overexpression of Lactobacillus helveticusd‐lactate dehydrogenase gene in Escherichia coli by KOCHHAR, Sunil, HOTTINGER, Herbert, CHUARD, Nathalie, TAYLOR, Paul G., ATKINSON, Tony, SCAWEN, Michael D., NICHOLLS, David J.

“…NAD+‐dependent d‐lactate dehydrogenase from Lactobacillus helveticus was purified to apparent homogeneity, and the sequence of the first 36 amino acid residues…”
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Molecular evolution of bacterial cell-surface proteins by Goward, C R, Scawen, M D, Murphy, J P, Atkinson, T

“…The cell-surface proteins of the infective bacteria Streptococcus and Staphylococcus are probably involved in the process of infection. These proteins share…”
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Cadmium-Binding Proteins in Pseudomonas putida: Pseudothioneins by Higham, Denise P., Sadler, Peter J., Scawen, Michael D.

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Overexpression of the Thermus aquaticus B malate dehydrogenase-encoding gene in Escherichia coli by Alldread, R M, Nicholls, D J, Sundaram, T K, Scawen, M D, Atkinson, T

“…Expression of the Thermus aquaticus B malate dehydrogenase (MDH)-encoding gene (mdh), cloned in Escherichia coli, was initially at a relatively low level (0.1%…”
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Cadmium Resistance in Pseudomonas putida: Growth and Uptake of Cadmium by HIGHAM, DENISE P, SADLER, PETER J, SCAWEN, MICHAEL D

“…1 Department of Chemistry, Birkbeck College, University of London, Malet Street, London WC1E 7HX, UK 2 Microbial Technology Laboratory, Centre for Applied…”
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Nucleotide sequence of the Erwinia chrysanthemi NCPPB 1066 L-asparaginase gene by Minton, N P, Bullman, H M, Scawen, M D, Atkinson, T, Gilbert, H J

“…The complete nucleotide sequence of the Erwinia chrysanthemi NCPPB 1066 gene coding for the chemotherapeutic enzyme L-asparaginase has been determined. The…”
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Purification and Properties of Aryol Acylamidase from Pseudomonas fluorescens ATCC 39004 by HAMMOND, Peter M., PRICE, Christopher P., SCAWEN, Michael D.

“…Aryl acytlamidase has been purified from a strain of Pseudomonas fluorescens ATCC 39004, selected from soil on the basis of its ability to utilise acylanilide…”
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A Single Amino Acid Mutation Enhances the Thermal Stability of Escherichia coli Malate Dehydrogenase by Goward, Christopher R., Miller, Julie, Nicholls, David J., Irons, Laurence I., Scawen, Michael D., O'brien, Ronan, Chowdhry, Babur Z.

“…The stability of wild‐type Escherichia coli malate dehydrogenase was compared with a mutant form of the enzyme with the amino acid residue at position 102…”
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Cloning and overexpression of Lactobacillus helveticus D-lactate dehydrogenase gene in Escherichia coli by KOCHHAR, S, HOTTINGER, H, CHUARD, N, TAYLOR, P. G, ATKINSON, T, SCAWEN, M. D, NICHOLLS, D. J

“…NAD(+)-dependent D-lactate dehydrogenase from Lactobacillus helveticus was purified to apparent homogeneity, and the sequence of the first 36 amino acid…”
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Large-scale protein extraction and isolation by Scawen, M D, Hammond, P M, Sherwood, R F, Atkinson, T

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Gold-resistant bacteria: excretion of a cystine-rich protein by Pseudomonas cepacia induced by an antiarthritic drug by Higham, D P, Sadler, P J, Scawen, M D

“…P. cepacia bacteria adapted to growth in a chemically defined medium containing millimolar concentrations of Au(I) thiolates including the antiarthritic drug…”
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Effect of Cadmium on the Morphology, Membrane Integrity and Permeability of Pseudomonas putida by Higham, Denise P, Sadler, Peter J, Scawen, Michael D

“…1 Department of Chemistry, Birkbeck College, University of London, Malet Street, London WC1E 7HX, UK 2 Microbial Technology Laboratory, Centre for Applied…”
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Rapid purification of glucokinase and glycerokinase from Bacillus stearothermophilus by hydrophobic interaction chromatography by Goward, C R, Atkinson, T, Scawen, M D

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The application of triazine dye affinity chromatography to the large-scale purification of glycerokinase from Bacillus stearothermophilus by Scawen, M D, Hammond, P M, Comer, M J, Atkinson, T

“…Gram quantities of homogeneous glycerokinase have been prepared from the thermophilic bacterium, Bacillus stearothermophilus, using three major steps:…”
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