Search Results - "Scawen, M D"

Erwinia chrysanthemi L-asparaginase : epitope mapping and production of antigenically modified enzymes by MOOLA, Z. B, SCWAEN, M. D, ATKINSON, T, NICHOLLS, D. J

“…This study shows that the antigenicity of Erwinia chrysanthemi L-asparaginase can be reduced by site-directed mutagenesis. Ten B-cell epitopes of the enzyme…”
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Contribution of a buried aspartate residue towards the catalytic efficiency and structural stability of Bacillus stearothermophilus lactate dehydrogenase by NOBBS, T. J, CORTES, A, GELPI, J. L, HOLBROOK, J. J, ATKINSON, T, SCAWEN, M. D, NICHOLLS, D. J

“…The X-ray structure of lactate dehydrogenase (LDH) shows the side-chain carboxylate group of Asp-143 to be buried in the hydrophobic interior of the enzyme,…”
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Catalytic-rate improvement of a thermostable malate dehydrogenase by a subtle alteration in cofactor binding by Alldread, R M, Halsall, D M, Clarke, A R, Sundaram, T K, Atkinson, T, Scawen, M D, Nicholls, D J

“…The nucleotide-binding fold of many NAD(+)-dependent dehydrogenases contains a conserved acidic amino acid residue which hydrogen-bonds with the 2'- and…”
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A study of the interactions between an IgG-binding domain based on the B domain of staphylococcal Protein A and rabbit IgG by BROWN, N. L, BOTTOMLEY, S. P, SCAWEN, M. D, GORE, M. G

“…The nonantigenic interaction between a recombinant immunoglobulin G (IgG)-binding protein based on the B domain of Protein A from Staphylococcus aureus (termed…”
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The importance of arginine 102 for the substrate specificity of Escherichia coli malate dehydrogenase by Nicholls, D J, Miller, J, Scawen, M D, Clarke, A R, Holbrook, J J, Atkinson, T, Goward, C R

“…The malate dehydrogenase from Escherichia coli has been specifically altered at a single amino acid residue by using site-directed mutagenesis. The conserved…”
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Cadmium-Resistant Pseudomonas putida Synthesizes Novel Cadmium Proteins by Higham, Denise P., Sadler, Peter J., Scawen, Michael D.

“…Three cysteine-rich proteins of molecular weight 4000 to 7000, containing 4 to 7 gram atoms of cadmium, zinc, and copper per mole were isolated from…”
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The identification of a structurally important cysteine residue in the glycerol dehydrogenase from Bacillus stearothermophilus by Spencer, P, Scawen, M D, Atkinson, T, Gore, M G

“…Evidence is presented to demonstrate that the Zn2+ metallo-enzyme glycerol dehydrogenase from the thermophile Bacillus stearothermophilus has one cysteine…”
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Effects of gold(I) antiarthritic drugs and related compounds on Pseudomonas putida by Rhodes, M D, Sadler, P J, Scawen, M D, Silver, S

“…The effects of the antiarthritic drugs aurothiomalate (AuTm), aurothioglucose (AuTg), auranofin, its metabolite triethylphosphinegold(I)thioglucose (Et3PAuTg),…”
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Identification of a reversible structural transition in the metal-depleted glycerol dehydrogenase from Bacillus stearothermophilus by Spencer, P., Paine, L.J., Scawen, M.D., Atkinson, T., Gore, M.G.

“…Evidence is presented to demonstrate that the Zn 2+-depleted, inactive form of the glycerol dehydrogenase from Bacillus stearothermophilus exists in one of two…”
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Separation and purification of oligonucleotides using a new bonded-phase packing material by Edwardson, P A, Collins, I J, Scawen, M D, Atkinson, T, Cox, G B, Sivakoff, S, Stout, R W

“…We describe a new bonded-phase packing material, based upon surface-stabilised microparticulate silica, suitable for the rapid separation and purification of…”
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The purification and characterization of glucokinase from the thermophile Bacillus stearothermophilus by Goward, C R, Hartwell, R, Atkinson, T, Scawen, M D

“…Homogeneous glucokinase (EC 2.7.1.2) from the thermophile Bacillus stearothermophilus was isolated on the large scale by using four major steps: precipitation…”
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Evaluation of an enzymic procedure for the measurement of acetaminophen by Price, C P, Hammond, P M, Scawen, M D

“…We evaluated a new method for measurement of acetaminophen. From acetaminophen, acetate and p-aminophenol are enzymically produced, and the latter product is…”
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Colorimetric glucose assay using thermostable glucokinase by Scott, D A, Goward, C R, Scawen, M D, Atkinson, T, Price, C P

“…A method for assaying glucose in serum or plasma samples using a thermostable glucokinase was developed. Glucokinase from Bacillus Stearothermophilus was…”
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Overexpression of the Thermus aquaticus B malate dehydrogenase-encoding gene in Escherichia coli by Alldread, R M, Nicholls, D J, Sundaram, T K, Scawen, M D, Atkinson, T

“…Expression of the Thermus aquaticus B malate dehydrogenase (MDH)-encoding gene (mdh), cloned in Escherichia coli, was initially at a relatively low level (0.1%…”
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Isolation and characterisation of the glycerol dehydrogenase from Bacillus stearothermophilus by Spencer, P, Bown, K J, Scawen, M D, Atkinson, T, Gore, M G

“…A protocol for the rapid purification of the glycerol dehydrogenase (glycerol: NAD+ 2-oxidoreductase, EC 1.1.1.6) from the thermophile Bacillus…”
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Cadmium Resistance in Pseudomonas putida: Growth and Uptake of Cadmium by HIGHAM, DENISE P, SADLER, PETER J, SCAWEN, MICHAEL D

“…1 Department of Chemistry, Birkbeck College, University of London, Malet Street, London WC1E 7HX, UK 2 Microbial Technology Laboratory, Centre for Applied…”
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Nucleotide sequence of the Erwinia chrysanthemi NCPPB 1066 L-asparaginase gene by Minton, N P, Bullman, H M, Scawen, M D, Atkinson, T, Gilbert, H J

“…The complete nucleotide sequence of the Erwinia chrysanthemi NCPPB 1066 gene coding for the chemotherapeutic enzyme L-asparaginase has been determined. The…”
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The inhibition of glucokinase and glycerokinase from Bacillus stearothermophilus by the triazine dye Procion Blue MX-3G by Goward, C R, Scawen, M D, Atkinson, T

“…Glucokinase from Bacillus stearothermophilus was irreversibly inactivated by the reactive dichlorotriazinyl dye Procion Blue MX-3G at pH 8.0. The enzyme was…”
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The rapid purification of 3-hydroxybutyrate dehydrogenase and malate dehydrogenase on triazine dye affinity matrices by Scawen, M D, Darbyshire, J, Harvey, M J, Atkinson, T

“…3-Hydroxybutyrate dehydrogenase (EC 1.1.1.30) and malate dehydrogenase (EC 1.1.1.37) were purified to homogeneity on a large scale involving only two…”
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Cloning and overexpression of Lactobacillus helveticus D-lactate dehydrogenase gene in Escherichia coli by KOCHHAR, S, HOTTINGER, H, CHUARD, N, TAYLOR, P. G, ATKINSON, T, SCAWEN, M. D, NICHOLLS, D. J

“…NAD(+)-dependent D-lactate dehydrogenase from Lactobacillus helveticus was purified to apparent homogeneity, and the sequence of the first 36 amino acid…”
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