Ribosomal DNA Restriction Analysis and Synthetic Oligonucleotide Probing in the Identification of Genera of Lower Trypanosomatids

Ribosomal DNA Restriction Analysis and Synthetic Oligonucleotide Probing in the Identification of Genera of Lower Trypanosomatids

Fifty-four species or isolates of insect trypanosomatids were examined for the presence of selected restriction enzyme sites in the small (SSU) and large (LSU) rRNA coding units of ribosomal genes. In the SSU, sites for Eco RI, Bgl II, Pst I, and Hind III were found to occur at the same location for...

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Bibliographic Details
Published in:The Journal of parasitology Vol. 78; no. 1; pp. 40 - 48
Main Authors: Camargo, Erney P., Sbravate, Clizete, Marta M. G. Teixeira, Silvia R. B. Uliana, Marcelo B. M. Soares, Affonso, Heloiza T., Floeter-Winter, Lucile
Format: Journal Article
Language:English
Published: United States American Society of Parasitologists 01-02-1992
Subjects:
Amidohydrolases - genetics
Animals
Antibodies, Monoclonal
Arginase - genetics
Base Sequence
Biological taxonomies
Biosystematics
Blotting, Southern
DNA probes
DNA Restriction Enzymes
DNA, Protozoan - genetics
DNA, Ribosomal - genetics
Enzymes
Genera
Molecular Sequence Data
Monoclonal antibodies
Oligonucleotide Probes
Oligonucleotides
Parasites
Parasitology
Ribosomal DNA
RNA, Ribosomal - genetics
RNA, Ribosomal, 18S - genetics
rRNA genes
Trypanosomatina - classification
Trypanosomatina - genetics
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Summary:Fifty-four species or isolates of insect trypanosomatids were examined for the presence of selected restriction enzyme sites in the small (SSU) and large (LSU) rRNA coding units of ribosomal genes. In the SSU, sites for Eco RI, Bgl II, Pst I, and Hind III were found to occur at the same location for all species examined, thus displaying a universal distribution among trypanosomatids. In the LSU, a site for Bgl II in the 24S-alpha sequence and sites for Hind III and Pst I in the 24S-beta sequence were found in all species examined. In contrast, a site for Pvu II in the SSU exhibited a genus-related distribution, being present in Crithidia and Herpetomonas but absent in Phytomonas. A site for Hind III in the 24S-alpha sequence of the LSU also exhibited genus-restricted distribution. The site was present in Crithidia but absent in Phytomonas and Herpetomonas. These findings were confirmed by dot hybridization with a synthetic oligonucleotide complementary to the 18S rRNA sequence containing the Pvu II site. Results point to the usefulness of restriction markers as diagnostic tools for distinguishing the lower trypanosomatid genera Crithidia, Herpetomonas, and Phytomonas at the same time revealing a marked complexity within the genus Leptomonas.
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ISSN:0022-3395
1937-2345
DOI:10.2307/3283683