Point-of-care testing of cholesterol and triglycerides for epidemiologic studies: evaluation of the multicare-in system

Cardiovascular disease is the leading cause of death in the world with 80% of cardiovascular events that occur in low- and middle-income countries. Reliable data on the prevalence of risk factors in developing countries can be obtained in door-to-door epidemiologic studies with the use of automatic...

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Published in:	Translational research : the journal of laboratory and clinical medicine Vol. 153; no. 2; pp. 71 - 76
Main Authors:	Rapi, Stefano, Bazzini, Cristina, Tozzetti, Camilla, Sbolci, Valentina, Modesti, Pietro Amedeo
Format:	Journal Article
Language:	English
Published:	New York, NY Mosby, Inc 01-02-2009 Elsevier
Subjects:	Adult Aged Biological and medical sciences Chemistry, Clinical - instrumentation Chemistry, Clinical - methods Chemistry, Clinical - standards Cholesterol - blood Dyslipidemias - diagnosis Dyslipidemias - epidemiology General aspects Humans Internal Medicine Investigative techniques, diagnostic techniques (general aspects) Medical sciences Middle Aged Point-of-Care Systems - standards Quality Control Sensitivity and Specificity Triglycerides - blood Young Adult point-of-care testing CV World Health Organization NCEP POCT CI ethylenediaminetetraacetic acid confidence interval National Cholesterol Education Program cardiovascular EDTA WHO Medicine Evaluation Point of care testing Clinical biology Lipids Triglyceride Epidemiology Cholesterol
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Summary:	Cardiovascular disease is the leading cause of death in the world with 80% of cardiovascular events that occur in low- and middle-income countries. Reliable data on the prevalence of risk factors in developing countries can be obtained in door-to-door epidemiologic studies with the use of automatic instruments. This study was performed to assess the sensitivity and specificity of a low-cost and manageable point-of-care testing (POCT) instrument (HPS MultiCare-in, Italy) for cholesterol and triglyceride assays. Plasma blood samples were obtained from consecutive subjects referred to our clinic for diagnostic evaluation. The analyzer currently used in our central laboratory (ADVIA 2400; Siemens, Deerfield, Ill) was used as comparison method. The inter-assay imprecision (expressed as variation coefficient) of the MultiCare POCT system was 4.51% (range, 2.38%–8.54%) and was 3.29% (range, 1.06%–7.45%) for cholesterol and triglycerides systems, respectively. The mean percent bias for capillary samples was 3.5 ± 4.3% for total cholesterol and –2.4 ± 4.9% for triglycerides. The difference in results obtained by nonprofessionals compared with professionals (practicability testing) was 0.28 ± 7.61% and 1.26 ± 9.86%, respectively ( P value was nonsignificant for both). Sensitivity and specificity measurements were 95.7% and 61.9% (threshold value of cholesterol 190 mg/dL) and 98% and 93.5% (threshold value of triglycerides 170 mg/dL), respectively. POCT instruments are essential to perform epidemiologic studies while avoiding transportation and storage of biologic material. The characteristics of sensitivity and specificity as well as diagnostic accuracy make the POCT instrument useful for obtaining an accurate stratification of a study population.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	1931-5244 1878-1810
DOI:	10.1016/j.trsl.2008.11.010