Search Results - "Sayers, J. R"

A helical arch allowing single-stranded DNA to thread through T5 5'-exonuclease by Ceska, T. A, Sayers, J. R, Stier, G, Suck, D

“…THE 5'-exonucleases are enzymes that are essential for DNA replication and repair. As well as their exonucleolytic action, removing nucleotides from the 5'-end…”
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Rapid high-efficiency site-directed mutagenesis by the phosphorothioate approach by Sayers, J R, Krekel, C, Eckstein, F

“…Several improvements to the existing phosphorothioate-based site-directed mutagenesis methodology are reported, and here it is demonstrated that the new…”
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Structure-Specific DNA Binding by Bacteriophage T5 5′→3′ Exonuclease by Garforth, Scott J., Sayers, Jon R.

“…Phage T5 exonuclease is a 5′→3′ exodeoxyribonuclease that also exhibits endonucleolytic activity on flap structures (branched duplex DNA containing a free…”
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Properties of overexpressed phage T5 D15 exonuclease. Similarities with Escherichia coli DNA polymerase I 5‘-3‘ exonuclease by Sayers, J R, Eckstein, F

“…The D15 gene of the bacteriophage T5, thought to encode an exonuclease, was cloned into an M13 phage on a 1344-base pair fragment. The deduced amino acid…”
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Schistosoma mansoni: anomalous immunogenic properties of a 27 kDa larval serine protease associated with protective immunity by Darani, H Y, Curtis, R H, McNeice, C, Price, H P, Sayers, J R, Doenhoff, M J

“…A cationic Schistosoma mansoni cercarial antigen was shown to be a serine protease as it was capable of hydrolysing N-acetyl-DL-phenylalanine beta-naphthyl…”
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Cloning, heterologous expression and antigenicity of a schistosome cercarial protease by Price, H P, Doenhoff, M J, Sayers, J R

“…A gene coding for the 30 kDa Schistosoma mansoni cercarial protease was amplified using the polymerase chain reaction (PCR) from genomic DNA templates. Cloning…”
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Two identical copies of IS1246, a 1275 base pair sequence related to other bacterial insertion sequences, enclose the xyl genes on TOL plasmid pWW0 by RAJASEKHAR REDDY, B, SHAW, L. E, SAYERS, J. R, WILLIAMS, P. A

“…Two identical direct repeats of a 1275 bp sequence, designated IS1246, encompass the xyl genes, which determine the catabolism of toluene, m- and p-xylenes to…”
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The evolution of pathways for aromatic hydrocarbon oxidation in Pseudomonas by WILLIAMS, P. A, SAYERS, J. R

“…The organisation and nucleotide sequences coding for the catabolism of benzene, toluene (and xylenes), naphthalene and biphenyl via catechol and the extradiol…”
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Immunoglobulin A1 proteases: a structure-function update by Parsons, H K, Vitovski, S, Sayers, J R

“…IgA1 (immunoglobulin A1) antibodies are the first line of defence against microbial pathogens such as Neisseria meningitidis and Haemophilus influenzae…”
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5'-3' exonucleases in phosphorothioate-based oligonucleotide-directed mutagenesis by SAYERS, J. R, SCHMIDT, W, ECKSTEIN, F

“…The application of T7 and lambda exonuclease to phosphorothioate-based oligonucleotide-directed mutagenesis was investigated. Oligonucleotide primers designed…”
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Preliminary crystallographic studies on the D15 5' to 3' exonuclease from phage T5 by Ceska, T A, Sayers, J R, Eckstein, F, Suck, D

“…The D15 exonuclease from phage T5 has been crystallized from 35% (w/v) ammonium sulfate by the hanging drop vapor diffusion technique. The crystals grow in…”
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Inhibition of the restriction endonuclease BanII using modified DNA substrates. Determination of phosphate residues critical for the formation of an active enzyme-DNA complex by OLSEN, D. B, KOTZOREK, G, SAYERS, J. R, ECKSTEIN, F

“…The restriction endonuclease BanII catalyzes the cleavage of double-stranded DNA and recognizes the degenerate sequence 5'-GPuGCPyC-3'. The poly-linker of…”
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Bacteriophage Collagen by Margaret C. M. Smith, Burns, Neil, Sayers, Jon R., Sorrell, Julian A., Casjens, Sherwood R., Hendrix, Roger W., Engel, Jurgen

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Mutagenesis of Conserved Lysine Residues in Bacteriophage T5 5′-3′Exonuclease Suggests Separate Mechanisms of Endo- and Exonucleolytic Cleavage by Garforth, Scott J., Ceska, Thomas A., Suck, Dietrich, Sayers, Jon R.

“…Efficient cellular DNA replication requires the activity of a 5′-3′exonuclease. These enzymes are able to hydrolyze$\text{DNA}· \text{DNA}$and$\text{RNA}·…”
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A single-strand specific endonuclease activity copurifies with overexpressed T5 D15 exonuclease by SAYERS, J. R, ECKSTEIN, F

“…The T5 D15 exonuclease purified from an overproducing strain of E. coli was shown to possess a low level of endonucleolytic activity specific for…”
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Continuous affinity-based selection: rapid screening and simultaneous amplification of bacterial surface-display libraries by Patel, D, Vitovski, S, Senior, H J, Edge, M D, Hockney, R C, Dempsey, M J, Sayers, J R

“…A new method for continuous biopanning has been developed. We have combined the power of affinity chromatography with the fecundity of bacteria in a unique…”
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Prokaryotic 5′-3′ exonucleases share a common core structure with gamma-delta resolvase by Artymiuk, Peter J., Ceska, Thomas A., Suck, Dietrich, Sayers, Jon R.

“…The three dimensional crystal structure of T5 5′-3′ exonuclease was compared with that of two other members of the 5′-3′ exonuclease family: T4 ribonuclease H…”
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Synthesis and antiviral properties of (E)-5-(2-bromovinyl)-2'-deoxycytidine-related compounds by Jones, A. Stanley, Sayers, Jon R, Walker, Richard T, De Clercq, Erik

“…Treatment of 3',5'-di-O-acetyl-(E)-5-(2-bromovinyl)-2'-deoxyuridine (2) with p-chlorophenyl phosphorodichloridate and 1,2,4-triazole gave…”
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Bilateral linear subconjunctival hemorrhage in a trauma patient by Rothouse, L S, Sayers, R J

“…A case of bilateral linear subconjunctival hemorrhage secondary to a gunshot wound to the chest has been presented. The linear character was explained by…”
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Inhibition of restriction endonuclease hydrolysis by phosphorothioate-containing DNA by SAYERS, J. R, OLSEN, D. B, ECKSTEIN, F

“…Phosphorothioate-containing M13 DNAs have been prepared as substrate analogues to study restriction endonuclease catalyzed hydrolysis. Approximately thirty…”
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