Plasma analysis of celiprolol by HPTLC: a useful technique for pharmacokinetic studies

A rapid and sensitive high performance, thin-layer chromatographic (HPTLC) method has been developed for the measurement of celiprolol in human plasma and its use in pharmacokinetic studies has been evaluated. Detection and quantitation were performed without using an internal standard. A simple ext...

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Bibliographic Details
Published in:	Journal of AOAC International Vol. 84; no. 4; pp. 1252 - 1257
Main Authors:	Savale, H S, Pandya, K K, Gandhi, T P, Modi, I A, Modi, R I, Satia, M C
Format:	Journal Article
Language:	English
Published:	England 01-07-2001
Subjects:	Adrenergic beta-Antagonists - blood Celiprolol - blood Celiprolol - pharmacokinetics Chromatography, Thin Layer Humans
Online Access:	Get full text
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Summary:	A rapid and sensitive high performance, thin-layer chromatographic (HPTLC) method has been developed for the measurement of celiprolol in human plasma and its use in pharmacokinetic studies has been evaluated. Detection and quantitation were performed without using an internal standard. A simple extraction procedure was followed for extracting celiprolol from plasma and a known amount of the extract was spotted on precoated silica gel 60 F254 plates using a Camag Linomat IV autosampler. Celiprolol was quantitated using a Camag TLC Scanner 3. The average recovery of authentic analytes (20 to 200 ng/mL) added to plasma was 72.06 +/- 2.8% and the lowest amount of celiprolol that could be detected was 10 ng/mL. The method provides a direct estimate of the amount of celiprolol present in plasma. Pharmacokinetic parameters of 2 marketed preparations have also been determined after oral administration to 12 healthy human volunteers.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1060-3271 1944-7922
DOI:	10.1093/jaoac/84.4.1252