Search Results - "Sasselli, Valentina"

Use of CRISPR-modified human stem cell organoids to study the origin of mutational signatures in cancer by Drost, Jarno, van Boxtel, Ruben, Blokzijl, Francis, Mizutani, Tomohiro, Sasaki, Nobuo, Sasselli, Valentina, de Ligt, Joep, Behjati, Sam, Grolleman, Judith E., van Wezel, Tom, Nik-Zainal, Serena, Kuiper, Roland P., Cuppen, Edwin, Clevers, Hans

“…Mutational processes underlie cancer initiation and progression. Signatures of these processes in cancer genomes may explain cancer etiology and could hold…”
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Functional Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients by Schwank, Gerald, Koo, Bon-Kyoung, Sasselli, Valentina, Dekkers, Johanna F., Heo, Inha, Demircan, Turan, Sasaki, Nobuo, Boymans, Sander, Cuppen, Edwin, van der Ent, Cornelis K., Nieuwenhuis, Edward E.S., Beekman, Jeffrey M., Clevers, Hans

“…Single murine and human intestinal stem cells can be expanded in culture over long time periods as genetically and phenotypically stable epithelial organoids…”
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Ascl2 Acts as an R-spondin/Wnt-Responsive Switch to Control Stemness in Intestinal Crypts by Schuijers, Jurian, Junker, Jan Philipp, Mokry, Michal, Hatzis, Pantelis, Koo, Bon-Kyoung, Sasselli, Valentina, van der Flier, Laurens G., Cuppen, Edwin, van Oudenaarden, Alexander, Clevers, Hans

“…The Wnt signaling pathway controls stem cell identity in the intestinal epithelium and in many other adult organs. The transcription factor Ascl2 (a Wnt target…”
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Retroviral gene expression control in primary organoid cultures by Koo, Bon-Kyoung, Sasselli, Valentina, Clevers, Hans

“…In this unit, we describe a protocol for regulated gene expression in primary endodermal organoid culture using retroviral vectors. The study of gene function…”
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Evaluation of ES-derived neural progenitors as a potential source for cell replacement therapy in the gut by Sasselli, Valentina, Micci, Maria-Adelaide, Kahrig, Kristen M, Pasricha, Pankaj Jay

“…Stem cell-based therapy has recently been explored for the treatment of disorders of the enteric nervous system (ENS). Pluripotent embryonic stem (ES) cells…”
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Developmental Biology Outstanding Paper Award

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The enteric nervous system by Sasselli, Valentina, Pachnis, Vassilis, Burns, Alan J.

“…The enteric nervous system (ENS), the intrinsic innervation of the gastrointestinal tract, consists of numerous types of neurons, and glial cells, that are…”
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Measuring mutation accumulation in single human adult stem cells by whole-genome sequencing of organoid cultures by Jager, Myrthe, Blokzijl, Francis, Sasselli, Valentina, Boymans, Sander, Janssen, Roel, Besselink, Nicolle, Clevers, Hans, van Boxtel, Ruben, Cuppen, Edwin

“…This protocol describes a method for cataloging genome-wide mutations that accumulated during life or culture in single adult stem cells of different human…”
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Tissue-specific mutation accumulation in human adult stem cells during life by Blokzijl, Francis, de Ligt, Joep, Jager, Myrthe, Sasselli, Valentina, Roerink, Sophie, Sasaki, Nobuo, Huch, Meritxell, Boymans, Sander, Kuijk, Ewart, Prins, Pjotr, Nijman, Isaac J., Martincorena, Inigo, Mokry, Michal, Wiegerinck, Caroline L., Middendorp, Sabine, Sato, Toshiro, Schwank, Gerald, Nieuwenhuis, Edward E. S., Verstegen, Monique M. A., van der Laan, Luc J. W., de Jonge, Jeroen, IJzermans, Jan N. M., Vries, Robert G., van de Wetering, Marc, Stratton, Michael R., Clevers, Hans, Cuppen, Edwin, van Boxtel, Ruben

“…Stem cells of the liver, colon and small intestine gradually accumulate mutations throughout life at a similar rate even though cancer incidence varies greatly…”
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Planar cell polarity genes control the connectivity of enteric neurons by Sasselli, Valentina, Boesmans, Werend, Vanden Berghe, Pieter, Tissir, Fadel, Goffinet, André M, Pachnis, Vassilis

“…A highly complex network of intrinsic enteric neurons is required for the digestive and homeostatic functions of the gut. Nevertheless, the genetic and…”
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