Biological control of Verticillium wilt of olive trees by using the biocontrol agent Paenibacillus alvei K-165

In the present work it was investigated the ability of the biocontrol agent K165 to protect olive trees against V. dahliae. For this purpose young olive trees of the susceptible to V. dahliae cv. Amfissis and the tolerant cv. Kalamon were planted in an artificial infested field. Sixteen months after...

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Published in:Phytopathologia mediterranea Vol. 48; no. 2; p. 348
Main Authors: Sassalou, L P, Markakis, E A, Tjamos, SE, Antoniou, P P, Paplomatas, E J, Tjamos, E C
Format: Journal Article
Language:English
Published: 01-08-2009
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Summary:In the present work it was investigated the ability of the biocontrol agent K165 to protect olive trees against V. dahliae. For this purpose young olive trees of the susceptible to V. dahliae cv. Amfissis and the tolerant cv. Kalamon were planted in an artificial infested field. Sixteen months after transplanting, (i) Verticillium wilt symptoms were recorded (ii) the biomass of V. dahliae in the xylem vessels of the trees was quantified by using the real time PCR technology and (iii) the viability of the pathogen in the plant tissues was confirmed by isolating the fungus on PDA plates. It was shown that in the K165 treated cv. Amfissis trees the disease percentage and the biomass of the fungus in the plant tissues was lower than in the control trees. On the other hand, the application of K165 in the resistant to Vertidllium wilt cv. Kalamon did not result in less symptom development and fungal biomass compared to the control trees.
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ISSN:0031-9465