Mesenchymal Stromal Cells Isolated from Ectopic but Not Eutopic Endometrium Display Pronounced Immunomodulatory Activity In Vitro

Mesenchymal Stromal Cells Isolated from Ectopic but Not Eutopic Endometrium Display Pronounced Immunomodulatory Activity In Vitro

A comparative analysis of the cell surface markers and immunological properties of cell cultures originating from normal endometrium and endometrioid heterotopias of women with extragenital endometriosis was carried out. Both types of cell cultures expressed surface molecules typical of mesenchymal...

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Published in:Biomedicines Vol. 9; no. 10; p. 1286
Main Authors: Lupatov, Alexey Yu, Saryglar, Roza Yu, Vtorushina, Valentina V., Poltavtseva, Rimma A., Bystrykh, Oxana A., Chuprynin, Vladimir D., Krechetova, Lyubov V., Pavlovich, Stanislav V., Yarygin, Konstantin N., Sukhikh, Gennady T.
Format: Journal Article
Language:English
Published: Basel MDPI AG 22-09-2021
MDPI
Subjects:
Antigens
Cell culture
Cell differentiation
Cell surface
Chemokines
Comparative analysis
Dendritic cells
Endometriosis
Endometrium
Immunomodulation
immunosuppression
Inflammation
Interleukin 6
Life sciences
Lymphocytes
Menstruation
Mesenchymal stem cells
mesenchymal stromal cells
Mesenchyme
Monocytes
Mutation
Pathogenesis
Phenotypes
Stem cells
Stromal cells
Surface markers
γ-Interferon
United States > US
Russia
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Summary:A comparative analysis of the cell surface markers and immunological properties of cell cultures originating from normal endometrium and endometrioid heterotopias of women with extragenital endometriosis was carried out. Both types of cell cultures expressed surface molecules typical of mesenchymal stromal cells and did not express hematopoietic and epithelial markers. Despite similar phenotype, the mesenchymal stromal cells derived from the two sources had different immunomodulation capacities: the cells of endometrioid heterotopias but not eutopic endometrium could suppress dendritic cell differentiation from monocytes as well as lymphocyte proliferation in allogeneic co-cultures. A comparative multiplex analysis of the secretomes revealed a significant increase in the secretion of pro-inflammatory mediators, including IL6, IFN-γ, and several chemokines associated with inflammation by the stromal cells of ectopic lesions. The results demonstrate that the stromal cells of endometrioid heterotopias display enhanced pro-inflammatory and immunosuppressive activities, which most likely impact the pathogenesis and progression of the disease.
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ISSN:2227-9059
2227-9059
DOI:10.3390/biomedicines9101286