Partition of proteins in aqueous two-phase systems based on Cashew-nut tree gum and poly(ethylene glycol)

The partitioning of two proteins, bovine serum albumin (BSA) and trypsin was studied in an aqueous poly(ethylene glycol)(PEG)- Cashew-nut tree gum system. The phase diagram was provided for Cashew-nut tree gum and PEG molecular weight of 1500 at two different temperatures. The influence of several p...

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Bibliographic Details
Published in:	Brazilian archives of biology and technology Vol. 47; no. 5; pp. 685 - 691
Main Authors:	Sarubbo, Leonie Asfora(Universidade Católica de Pernambuco Departamento de Química), Oliveira, Luciana Alves de(UNICAMP FEQ DESQ), Porto, Ana Lúcia Figueiredo(UFPE Laboratório de Imunopatologia Keizo Asami ,UFRPE Departamento de Morfologia e Fisiologia Animal), Campos-Takaki, Galba Maria de(Universidade Católica de Pernambuco Departamento de Química), Tambourgi, Elias Basile(UNICAMP FEQ DESQ)
Format:	Journal Article
Language:	English
Published:	Tecpar 01-09-2004 Instituto de Tecnologia do Paraná (Tecpar)
Subjects:	Aqueous two-phase systems Cashew-nut tree gum ine serum albumin protein partition
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Summary:	The partitioning of two proteins, bovine serum albumin (BSA) and trypsin was studied in an aqueous poly(ethylene glycol)(PEG)- Cashew-nut tree gum system. The phase diagram was provided for Cashew-nut tree gum and PEG molecular weight of 1500 at two different temperatures. The influence of several parameters including concentrations of polymers, pH, salt addition and temperature on the partitioning of these proteins were investigated.. The results of this research demonstrated the importance of the protein characteristics for partitioning in aqueous biphasic system. A partição de duas proteínas, albumina de soro bovino (BSA) e tripsina foi estudada no sistema bifásico aquoso Polietileno glicol(PEG) - Goma do cajueiro. O diagrama de fases foi estabelecido para a Goma do Cajueiro e para PEG de peso molecular 1500 em duas diferentes temperaturas. A influência de vários parâmetros na partição destas proteínas, incluindo concentração dos polímeros, pH, adição de sal e temperatura foi investigada. Os resultados desta pesquisa demonstraram a importância das características da proteína na partição em sistemas bifásicos aquosos.
Bibliography:	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500003 10.1590/S1516-89132004000500003
ISSN:	1516-8913 1516-8913 1678-4324
DOI:	10.1590/S1516-89132004000500003