Insights on mining the pangenome of Sphingobacterium thalpophilum NMS02 S296 from the resistant banana cultivar Pisang lilin confirms the antifungal action against Fusarium oxysporum f. sp. cubense
wilt, caused by f. sp. ( ), poses a significant global threat to banana cultivation. Conventional methods of disease management are increasingly challenged, thus making it necessary to explore alternative strategies. Bacterial endophytes, particularly from resistant genotypes, are gaining attention...
Saved in:
| Published in: | Frontiers in microbiology Vol. 15; p. 1443195 |
|---|---|
| Main Authors: | , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Switzerland
Frontiers Media S.A
19-09-2024
|
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | wilt, caused by
f. sp.
(
), poses a significant global threat to banana cultivation. Conventional methods of disease management are increasingly challenged, thus making it necessary to explore alternative strategies. Bacterial endophytes, particularly from resistant genotypes, are gaining attention as potential biocontrol agents.
, isolated from the resistant banana cultivar
(JALHSB010000001-JALHSB010000029), presents an intriguing prospect for combating
wilt. However, its underlying biocontrol mechanisms remain poorly understood. This study aimed to elucidate the antifungal efficacy of
NMS02 S296 against
and explore its biocontrol mechanisms at the genomic level.
Whole genome sequencing of
NMS02 S296 was conducted using next-generation sequencing technologies and bioinformatics analyses were performed to identify genes associated with antifungal properties.
assays were used to assess the inhibitory effects of the bacterial isolate on the mycelial growth of
. To explore the biomolecules responsible for the observed antagonistic activity, metabolites diffused into the agar at the zone of inhibition between
S16 and
NMS02 S296 were extracted and identified.
Whole genome sequencing revealed an array of genes encoding antifungal enzymes and secondary metabolites in
NMS02 S296. In vitro experiments demonstrated significant inhibition of
mycelial growth by the bacterial endophyte. Comparative genomic analysis highlighted unique genomic features in
linked to its biocontrol potential, setting it apart from other bacterial species.
The study underscores the remarkable antifungal efficacy of
NMS02 S296 against
wilt. The genetic basis for its biocontrol potential was elucidated through whole genome sequencing, shedding light on the mechanisms behind its antifungal activity. This study advanced our understanding of bacterial endophytes as biocontrol agents and offers a promising avenue for plant growth promotion towards sustainable strategies to mitigate
wilt in banana cultivation. |
|---|---|
| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Kusum Dhakar, Independent Researcher, New Delhi, India Cesar Hugo Hernández-Rodríguez, Instituto Politécnico Nacional, Mexico Edited by: José David Flores Félix, University of Salamanca, Spain |
| ISSN: | 1664-302X 1664-302X |
| DOI: | 10.3389/fmicb.2024.1443195 |