Rapid Self-Assembly of Macroscale Tissue Constructs at Biphasic Aqueous Interfaces

An entirely new approach to tissue engineering is presented that uses the interfacial forces between aqueous solutions of phase‐separating polymers to confine cells and promote their assembly into interconnected, macroscopic tissue constructs. This simple and inexpensive general procedure creates fr...

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Bibliographic Details
Published in:	Advanced functional materials Vol. 25; no. 11; pp. 1694 - 1699
Main Authors:	Frampton, John P., Leung, Brendan M., Bingham, Eve L., Lesher-Perez, Sasha Cai, Wang, Jack D., Sarhan, Hady T., El-Sayed, Mohamed E. H., Feinberg, Stephen E., Takayama, Shuichi
Format:	Journal Article
Language:	English
Published:	Blackwell Publishing Ltd 01-03-2015
Subjects:	3D culture aqueous two-phase system Assembly Construction Dextrans Equivalence In vitro testing Integrity interfaces Polymers Self assembly tissue constructs
Online Access:	Get full text
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Summary:	An entirely new approach to tissue engineering is presented that uses the interfacial forces between aqueous solutions of phase‐separating polymers to confine cells and promote their assembly into interconnected, macroscopic tissue constructs. This simple and inexpensive general procedure creates free‐standing, centimeter‐scale constructs from cell suspensions at the interface between poly(ethylene glycol) and dextran aqueous two‐phase systems in as little as 2 h. Using this method, skin constructs are produced that integrate with decellularized dermal matrices, on which they differentiate and stratify into skin equivalents. It is demonstrated that the constructs produced by this method have appropriate integrity and mechanical properties for use as in vitro tissue models. Macroscopic tissue constructs composed entirely of cells are formed using the interfacial properties of aqueous two‐phase systems. The constructs form rapidly in as little as 2 h using a variety of cell types, offering a new methodology for fabricating tissue‐engineered in vitro models and cell‐based materials for regenerative therapies.
Bibliography:	Coulter Foundation Naval Warfare Systems Center Pacific - No. N6601-13-C-2027 ArticleID:ADFM201403825 Defense Threat Reduction Agency ark:/67375/WNG-PNTFFGJ9-T istex:487D98668ABECF0D91461CB707C2AD71CE397E3B ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1616-301X 1616-3028
DOI:	10.1002/adfm.201403825