Nanoparticle-Mediated Signaling for Aptamer-Based Multiplexed Detection of Cortisol and Neuropeptide Y in Serum

Nanoparticle-Mediated Signaling for Aptamer-Based Multiplexed Detection of Cortisol and Neuropeptide Y in Serum

Multiplexed profiling of the expression of neurochemical biomarkers of stress, for periodic assessment to enable augmentation of human performance, requires wash-free detection platforms that exhibit reproducible signals from samples in biological matrices. However, alterations in aptamer conformati...

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Bibliographic Details
Published in:Chemosensors Vol. 10; no. 5; p. 153
Main Authors: Sardesai, Naimesh, Liu, Yi, Shia, Regina, Mirau, Peter, Chávez, Jorge L., Swami, Nathan S.
Format: Journal Article
Language:English
Published: Basel MDPI AG 01-05-2022
Subjects:
aptamer
Aptamers
Augmentation
Biomarkers
Conformation
Cortisol
Electrodes
Gold
Hormones
Human performance
Methylene blue
microfluidics
Multiplexing
nanoparticle
Nanoparticles
Neuropeptide Y
Neuropeptides
NMR
Nuclear magnetic resonance
Physiology
Proteins
Semiconductors
Sensors
Serum proteins
Signaling
Spectrum analysis
stress
Target recognition
voltammetry
United States > US
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Summary:Multiplexed profiling of the expression of neurochemical biomarkers of stress, for periodic assessment to enable augmentation of human performance, requires wash-free detection platforms that exhibit reproducible signals from samples in biological matrices. However, alterations in aptamer conformation after binding to targets, such as cortisol, are minimal based on NMR spectra, and the methylene blue signaling is blocked by serum proteins. Hence, in this study, we explore aptamer derivatization with magnetic nanoparticles that are conjugated with multiple methylene blue moieties, to amplify signals and alter the net charge configuration for repulsing serum proteins, so that the aptamer conformation upon target recognition can lead to a signal ON assay in serum media. Based on this, a microchip platform with addressable electrodes that are immobilized with selective aptamer receptors is developed for multiplexed detection of cortisol (1–700 ng/mL) and neuropeptide Y (5–1000 pg/mL) in patient-derived serum samples, which is validated by immunoassays. We envision the application of this sensor for profiling a wider array of human performance biomarkers under stress-related events to develop stress augmentation methodologies.
ISSN:2227-9040
2227-9040
DOI:10.3390/chemosensors10050153