Probiotic properties of lactic acid bacteria isolated from human milk

Aim The objective of this study was to identify and characterize lactic acid bacteria isolated from human milk, with an emphasis on their probiotic properties. Methods and Results The strains were tested for their ability to inhibit growth of Enterococcus faecalis, Salmonella enterica subsp. enteric...

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Bibliographic Details
Published in:	Journal of applied microbiology Vol. 121; no. 3; pp. 811 - 820
Main Authors:	Reis, N.A., Saraiva, M.A.F., Duarte, E.A.A., Carvalho, E.A., Vieira, B.B., Evangelista‐Barreto, N.S.
Format:	Journal Article
Language:	English
Published:	England Oxford University Press 01-09-2016
Subjects:	Acids antagonism Antimicrobial agents Bacteria Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Bile Bile salts Breast milk Breastfeeding & lactation Catalase E coli Enterococcus Enterococcus faecalis Escherichia coli Female Gene sequencing Gentamicin human milk Humans Lactic acid Lactic Acid - metabolism Lactic acid bacteria Listeria Listeria monocytogenes Milk Milk, Human - microbiology Nursing pH effects Probiotics Probiotics - classification Probiotics - isolation & purification Probiotics - metabolism Properties (attributes) rRNA 16S Salmonella Salmonella enterica Salt tolerance Salts Staphylococcus aureus Vancomycin Weissella confusa Yogurt Enterococcus human milk antagonism lactic acid bacteria probiotics
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Summary:	Aim The objective of this study was to identify and characterize lactic acid bacteria isolated from human milk, with an emphasis on their probiotic properties. Methods and Results The strains were tested for their ability to inhibit growth of Enterococcus faecalis, Salmonella enterica subsp. enterica serotype Enteritidis, Listeria monocytogenes, Staphylococcus aureus and Escherichia coli, as well as for susceptibility to antimicrobial agents and for acid pH and bile salt tolerance. Gram‐positive and catalase‐negative were selected and identified as Enterococcus (83·3%) after sequencing the 16S rDNA gene. All the isolates inhibited growth of Ent. faecalis and S. serotype Enteritidis, 97% inhibited growth of L. monocytogenes and Staph. aureus and 78·8% inhibited growth of E. coli. Most of the isolates were resistant to gentamicin (50%) and vancomycin (47%). Twelve isolates grew when subjected to pH 3·0 and 0·1% bile salts. At lower pH (2·5–2·0), Ent. faecalis F1 and Weissella confusa F8 were more efficient. Conclusion It was possible to isolate from human milk the lactic acid bacteria with potential for use as probiotics. Significance and Impact of the Study Lactic acid bacteria isolated of nursing mothers have probiotic properties.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1364-5072 1365-2672
DOI:	10.1111/jam.13173