Search Results - "Sarah W.,Harcum"

High extracellular lactate causes reductive carboxylation in breast tissue cell lines grown under normoxic conditions by Brodsky, Arthur Nathan, Odenwelder, Daniel C, Harcum, Sarah W

“…In cancer tumors, lactate accumulation was initially attributed to high glucose consumption associated with the Warburg Effect. Now it is evident that lactate…”
Get full text

Microevolutionary dynamics of eccDNA in Chinese hamster ovary cells grown in fed-batch cultures under control and lactate-stressed conditions by Chitwood, Dylan G., Wang, Qinghua, Klaubert, Stephanie R., Green, Kiana, Wu, Cathy H., Harcum, Sarah W., Saski, Christopher A.

“…Chinese hamster ovary (CHO) cell lines are widely used to manufacture biopharmaceuticals. However, CHO cells are not an optimal expression host due to the…”
Get full text

Stochastic biological system-of-systems modelling for iPSC culture by Zheng, Hua, Harcum, Sarah W., Pei, Jinxiang, Xie, Wei

“…Large-scale manufacturing of induced pluripotent stem cells (iPSCs) is essential for cell therapies and regenerative medicines. Yet, iPSCs form large cell…”
Get full text

Characterization of metabolic responses, genetic variations, and microsatellite instability in ammonia-stressed CHO cells grown in fed-batch cultures by Chitwood, Dylan G, Wang, Qinghua, Elliott, Kathryn, Bullock, Aiyana, Jordana, Dwon, Li, Zhigang, Wu, Cathy, Harcum, Sarah W, Saski, Christopher A

“…As bioprocess intensification has increased over the last 30 years, yields from mammalian cell processes have increased from 10's of milligrams to over 10's of…”
Get full text

Machine Learning and Deep Learning Strategies for Chinese Hamster Ovary Cell Bioprocess Optimization by Baako, Tiffany-Marie D, Kulkarni, Sahil Kaushik, McClendon, Jerome L, Harcum, Sarah W, Gilmore, Jordon

“…The use of machine learning and deep learning has become prominent within various fields of bioprocessing for countless modeling and prediction tasks. Previous…”
Get full text

Metabolic regulatory network kinetic modeling with multiple isotopic tracers for iPSCs by Wang, Keqi, Xie, Wei, Harcum, Sarah W.

“…The rapidly expanding market for regenerative medicines and cell therapies highlights the need to advance the understanding of cellular metabolisms and improve…”
Get full text

Effects of amino acid additions on ammonium stressed CHO cells by Chen, Peifeng, Harcum, Sarah W.

“…Ammonium is a toxic and inhibitory byproduct of mammalian cell metabolism. At the end of a typical recombinant protein production campaign, the ammonium…”
Get full text

Recombinant protein production in an Escherichia coli reduced genome strain by Sharma, Shamik S., Blattner, Frederick R., Harcum, Sarah W.

“…Recently, efforts have been made to improve the properties of Escherichia coli as a recombinant host by ‘genomic surgery’—deleting large segments of the E…”
Get full text

Editorial overview: Pharmaceutical biotechnology: the pandemic years — new modality development and biomanufacturing innovations in a maturing field by Harcum, Sarah W, Kiss, Robert D

Get full text

Sensitive real-time on-line estimator for oxygen transfer rates in fermenters by Trout, Marshall, Harcum, Sarah W., Groff, Richard E.

“…Recombinant Escherichia coli grown in large-scale fermenters are used extensively to produce plasmids and biopharmaceuticals. One method commonly used to…”
Get full text

Induced pluripotent stem cells can utilize lactate as a metabolic substrate to support proliferation by Odenwelder, Daniel C., Lu, Xiaoming, Harcum, Sarah W.

“…Human‐induced pluripotent stem cells (iPSCs) hold the promise to improve cell‐based therapies. Yet, to meet rising demands and become clinically impactful,…”
Get full text

In-line buffer exchange in the coupling of Protein A chromatography with weak cation exchange chromatography for the determination of charge variants of immunoglobulin G derived from chinese hamster ovary cell cultures by Wysor, Sarah K., Synoground, Benjamin F, Harcum, Sarah W., Marcus, R. Kenneth

“…•The solvents which effect elution in ProA and WCX chromatography are incompatible.•Solvent exchange is needed for 2DLC charge variant analysis of mAbs via…”
Get full text

Global transcriptome response of recombinant Escherichia coli to heat-shock and dual heat-shock recombinant protein induction by HARCUM, Sarah W, HADDADIN, Fuad T

“…Recombinant Escherichia coli cultures are used to manufacture numerous therapeutic proteins and industrial enzymes, where many of these processes use elevated…”
Get full text

Microwave Sensing of Yeast Cell Species and Viability by Osterberg, Jeffrey A., Dahal, Neelima, Divan, Ralu, Miller, Christina S., Moline, David, Caldwell, Thomas P., Yu, Xianzhong, Harcum, Sarah W., Wang, Pingshan

“…We report the development of a simple interferometer-based microwave sensing system for multiple frequency characterization and differentiation of in-flow…”
Get full text

Expression of two recombinant chloramphenicol acetyltransferase variants in highly reduced genome Escherichia coli strains by Sharma, Shamik S, Campbell, John W, Frisch, David, Blattner, Frederick R, Harcum, Sarah W

“…Highly reduced E. coli strains, MDS40, MDS41, and MDS42, lacking approximately 15% of the genome, were grown to high cell densities to test their ability to…”
Get full text

Low glucose concentrations within typical industrial operating conditions have minimal effect on the transcriptome of recombinant CHO cells by Gowtham, Yogender Kumar, Saski, Christopher A., Harcum, Sarah W.

“…Typically, mammalian cell culture medium contains high glucose concentrations that are analogous to diabetic levels in humans, suggesting that mammalian cells…”
Get full text

Transient ammonia stress on Chinese hamster ovary (CHO) cells yield alterations to alanine metabolism and IgG glycosylation profiles by Synoground, Benjamin F., McGraw, Claire E., Elliott, Kathryn S., Leuze, Christina, Roth, Jada R., Harcum, Sarah W., Sandoval, Nicholas R.

“…Background Ammonia concentrations typically increase during mammalian cell cultures, mainly due to glutamine and other amino acid consumption. An early ammonia…”
Get full text

Generation of reference cell lines, media, and a process platform for CHO cell biomanufacturing by Cordova, Lauren T., Dahodwala, Hussain, Elliott, Kathryn S., Baik, Jongyoun, Odenewelder, Daniel C., Nmagu, Douglas, Skelton, Bradley A., Uy, Lisa, Klaubert, Stephanie R., Synoground, Benjamin F., Chitwood, Dylan G., Dhara, Venkata Gayatri, Naik, Harnish Mukesh, Morris, Caitlin S., Yoon, Seongkyu, Betenbaugh, Michael, Coffman, Jon, Swartzwelder, Frank, Gillmeister, Michael P., Harcum, Sarah W., Lee, Kelvin H.

“…Due to the favorable attributes of Chinese hamster ovary (CHO) cells for therapeutic proteins and antibodies biomanufacturing, companies generate proprietary…”
Get full text

Dynamics of Amino Acid Metabolism, Gene Expression, and Circulomics in a Recombinant Chinese Hamster Ovary Cell Line Adapted to Moderate and High Levels of Extracellular Lactate by Chitwood, Dylan G, Uy, Lisa, Fu, Wanfang, Klaubert, Stephanie R, Harcum, Sarah W, Saski, Christopher A

“…The accumulation of metabolic wastes in cell cultures can diminish product quality, reduce productivity, and trigger apoptosis. The limitation or removal of…”
Get full text

Antibody purification from CHO cell supernatant using new multimodal membranes by Wang, Juan, Zhou, Jinxiang, Gowtham, Yogender K., Harcum, Sarah W., Husson, Scott M.

“…This contribution describes strategies to purify monoclonal antibodies from Chinese hamster ovary (CHO) cell culture supernatant using newly designed…”
Get full text