Search Results - "Sanz, Lionel A."

High-resolution, strand-specific R-loop mapping via S9.6-based DNA–RNA immunoprecipitation and high-throughput sequencing by Sanz, Lionel A., Chédin, Frédéric

“…R-loops are prevalent three-stranded non-B DNA structures composed of an RNA–DNA hybrid and a single strand of DNA. R-loops are implicated in various basic…”
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Prevalent, Dynamic, and Conserved R-Loop Structures Associate with Specific Epigenomic Signatures in Mammals by Sanz, Lionel A., Hartono, Stella R., Lim, Yoong Wearn, Steyaert, Sandra, Rajpurkar, Aparna, Ginno, Paul A., Xu, Xiaoqin, Chédin, Frédéric

“…R-loops are three-stranded nucleic acid structures formed upon annealing of an RNA strand to one strand of duplex DNA. We profiled R-loops using a…”
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Recognition of RNA by the S9.6 antibody creates pervasive artifacts when imaging RNA:DNA hybrids by Smolka, John A, Sanz, Lionel A, Hartono, Stella R, Chédin, Frédéric

“…The S9.6 antibody is broadly used to detect RNA:DNA hybrids but has significant affinity for double-stranded RNA. The impact of this off-target RNA binding…”
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Transcription-associated R-loop formation across the human FMR1 CGG-repeat region by Loomis, Erick W, Sanz, Lionel A, Chédin, Frédéric, Hagerman, Paul J

“…Expansion of a trinucleotide (CGG) repeat element within the 5' untranslated region (5'UTR) of the human FMR1 gene is responsible for a number of heritable…”
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DNA Topoisomerase I differentially modulates R-loops across the human genome by Manzo, Stefano G, Hartono, Stella R, Sanz, Lionel A, Marinello, Jessica, De Biasi, Sara, Cossarizza, Andrea, Capranico, Giovanni, Chedin, Frederic

“…Co-transcriptional R-loops are abundant non-B DNA structures in mammalian genomes. DNA Topoisomerase I (Top1) is often thought to regulate R-loop formation…”
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Co-transcriptional R-loops are the main cause of estrogen-induced DNA damage by Stork, Caroline Townsend, Bocek, Michael, Crossley, Madzia P, Sollier, Julie, Sanz, Lionel A, Chédin, Frédéric, Swigut, Tomek, Cimprich, Karlene A

“…The hormone estrogen (E2) binds the estrogen receptor to promote transcription of E2-responsive genes in the breast and other tissues. E2 also has links to…”
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Genome-wide DNA hypomethylation and RNA:DNA hybrid accumulation in Aicardi-Goutières syndrome by Lim, Yoong Wearn, Sanz, Lionel A, Xu, Xiaoqin, Hartono, Stella R, Chédin, Frédéric

“…Aicardi-Goutières syndrome (AGS) is a severe childhood inflammatory disorder that shows clinical and genetic overlap with systemic lupus erythematosus (SLE)…”
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RNA-DNA strand exchange by the Drosophila Polycomb complex PRC2 by Alecki, Célia, Chiwara, Victoria, Sanz, Lionel A., Grau, Daniel, Arias Pérez, Osvaldo, Boulier, Elodie L., Armache, Karim-Jean, Chédin, Frédéric, Francis, Nicole J.

“…Polycomb Group (PcG) proteins form memory of transient transcriptional repression that is necessary for development. In Drosophila , DNA elements termed…”
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EHMT2 directs DNA methylation for efficient gene silencing in mouse embryos by Auclair, Ghislain, Borgel, Julie, Sanz, Lionel A, Vallet, Judith, Guibert, Sylvain, Dumas, Michael, Cavelier, Patricia, Girardot, Michael, Forné, Thierry, Feil, Robert, Weber, Michael

“…The extent to which histone modifying enzymes contribute to DNA methylation in mammals remains unclear. Previous studies suggested a link between the lysine…”
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Histone methylation is mechanistically linked to DNA methylation at imprinting control regions in mammals by Henckel, Amandine, Nakabayashi, Kazuhiko, Sanz, Lionel A., Feil, Robert, Hata, Kenichiro, Arnaud, Philippe

“…Mono-allelic expression of imprinted genes from either the paternal or the maternal allele is mediated by imprinting control regions (ICRs), which are…”
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A mono-allelic bivalent chromatin domain controls tissue-specific imprinting at Grb10 by Sanz, Lionel A, Chamberlain, Stormy, Sabourin, Jean-Charles, Henckel, Amandine, Magnuson, Terry, Hugnot, Jean-Philippe, Feil, Robert, Arnaud, Philippe

“…Genomic imprinting is a developmental mechanism that mediates parent‐of‐origin‐specific expression in a subset of genes. How the tissue specificity of…”
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Best practices for the visualization, mapping, and manipulation of R‐loops by Chédin, Frédéric, Hartono, Stella R, Sanz, Lionel A, Vanoosthuyse, Vincent

“…R‐loops represent an abundant class of large non‐B DNA structures in genomes. Even though they form transiently and at modest frequencies, interfering with…”
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Ultra-deep Coverage Single-molecule R-loop Footprinting Reveals Principles of R-loop Formation by Malig, Maika, Hartono, Stella R., Giafaglione, Jenna M., Sanz, Lionel A., Chedin, Frederic

“…R-loops are a prevalent class of non-B DNA structures that have been associated with both positive and negative cellular outcomes. DNA:RNA immunoprecipitation…”
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The Air Noncoding RNA Epigenetically Silences Transcription by Targeting G9a to Chromatin by Nagano, Takashi, Mitchell, Jennifer A., Sanz, Lionel A., Pauler, Florian M., Ferguson-Smith, Anne C., Feil, Robert, Fraser, Peter

“…A number of large noncoding RNAs (ncRNAs) epigenetically silence genes through unknown mechanisms. The Air ncRNA is imprinted--monoallelically expressed from…”
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SETX (senataxin), the helicase mutated in AOA2 and ALS4, functions in autophagy regulation by Richard, Patricia, Feng, Shuang, Tsai, Yueh-Lin, Li, Wencheng, Rinchetti, Paola, Muhith, Ubayed, Irizarry-Cole, Juan, Stolz, Katharine, Sanz, Lionel A., Hartono, Stella, Hoque, Mainul, Tadesse, Saba, Seitz, Hervé, Lotti, Francesco, Hirano, Michio, Chédin, Frédéric, Tian, Bin, Manley, James L.

“…SETX (senataxin) is an RNA/DNA helicase that has been implicated in transcriptional regulation and the DNA damage response through resolution of R-loop…”
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Genomic patterns of transcription–replication interactions in mouse primary B cells by St Germain, Commodore P, Zhao, Hongchang, Sinha, Vrishti, Sanz, Lionel A, Chédin, Frédéric, Barlow, Jacqueline H

“…Abstract Conflicts between transcription and replication machinery are a potent source of replication stress and genome instability; however, no technique…”
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CARM1 Inhibition Enables Immunotherapy of Resistant Tumors by Dual Action on Tumor Cells and T Cells by Kumar, Sushil, Zeng, Zexian, Bagati, Archis, Tay, Rong En, Sanz, Lionel A, Hartono, Stella R, Ito, Yoshinaga, Abderazzaq, Fieda, Hatchi, Elodie, Jiang, Peng, Cartwright, Adam N R, Olawoyin, Olamide, Mathewson, Nathan D, Pyrdol, Jason W, Li, Mamie Z, Doench, John G, Booker, Matthew A, Tolstorukov, Michael Y, Elledge, Stephen J, Chédin, Frédéric, Liu, X Shirley, Wucherpfennig, Kai W

“…A number of cancer drugs activate innate immune pathways in tumor cells but unfortunately also compromise antitumor immune function. We discovered that…”
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PRMT1-dependent regulation of RNA metabolism and DNA damage response sustains pancreatic ductal adenocarcinoma by Giuliani, Virginia, Miller, Meredith A., Liu, Chiu-Yi, Hartono, Stella R., Class, Caleb A., Bristow, Christopher A., Suzuki, Erika, Sanz, Lionel A., Gao, Guang, Gay, Jason P., Feng, Ningping, Rose, Johnathon L., Tomihara, Hideo, Daniele, Joseph R., Peoples, Michael D., Bardenhagen, Jennifer P., Geck Do, Mary K., Chang, Qing E., Vangamudi, Bhavatarini, Vellano, Christopher, Ying, Haoqiang, Deem, Angela K., Do, Kim-Anh, Genovese, Giannicola, Marszalek, Joseph R., Kovacs, Jeffrey J., Kim, Michael, Fleming, Jason B., Guccione, Ernesto, Viale, Andrea, Maitra, Anirban, Emilia Di Francesco, M., Yap, Timothy A., Jones, Philip, Draetta, Giulio, Carugo, Alessandro, Chedin, Frederic, Heffernan, Timothy P.

“…Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer that has remained clinically challenging to manage. Here we employ an RNAi-based in vivo…”
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Genome-wide DNA demethylation in mammals by Sanz, Lionel A, Kota, Satya K, Feil, Robert

“…The cytidine deaminase AID and elongator-complex proteins contribute to the extensive removal of DNA methylation in mammalian primordial germ cells and in the…”
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Topoisomerase 1 prevents replication stress at R-loop-enriched transcription termination sites by Promonet, Alexy, Padioleau, Ismaël, Liu, Yaqun, Sanz, Lionel, Biernacka, Anna, Schmitz, Anne-Lyne, Skrzypczak, Magdalena, Sarrazin, Amélie, Mettling, Clément, Rowicka, Maga, Ginalski, Krzysztof, Chedin, Frédéric, Chen, Chun-Long, Lin, Yea-Lih, Pasero, Philippe

“…R-loops have both positive and negative impacts on chromosome functions. To identify toxic R-loops in the human genome, here, we map RNA:DNA hybrids,…”
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