Association between the thrombophilic polymorphisms MTHFR C677T, Factor V Leiden, and prothrombin G20210A and recurrent miscarriage in Brazilian women

Some cases of recurrent first trimester miscarriage have a thrombotic etiology. The aim of this study was to investigate the prevalence of the most common thrombophilic mutations - factor V (FV) Leiden G1691A (FVL), prothrombin (FII) G20210A, and methylenetetrahydrofolate reductase (MTHFR) C677T - i...

Full description

Saved in:

Bibliographic Details
Published in:	Genetics and molecular research Vol. 15; no. 3
Main Authors:	Gonçalves, R O, Fraga, L R, Santos, W V B, Carvalho, A F L, Veloso Cerqueira, B A V, Sarno, M, Toralles, M B P, Vieira, M J, Dutra, C G, Schüler-Faccini, L, Sanseverino, M T V, Gonçalves, M S, Vianna, F S L, Costa, O L N
Format:	Journal Article
Language:	English
Published:	Brazil 15-07-2016
Subjects:	Abortion, Habitual - genetics Adult Case-Control Studies Factor V - genetics Female Gene Frequency Humans Methylenetetrahydrofolate Reductase (NADPH2) - genetics Polymorphism, Restriction Fragment Length Pregnancy Prothrombin - genetics
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Some cases of recurrent first trimester miscarriage have a thrombotic etiology. The aim of this study was to investigate the prevalence of the most common thrombophilic mutations - factor V (FV) Leiden G1691A (FVL), prothrombin (FII) G20210A, and methylenetetrahydrofolate reductase (MTHFR) C677T - in women with recurrent miscarriages. In this case-control study, we included 137 women with two or more consecutive first-trimester miscarriages (£12 weeks of gestation) and 100 healthy women with no history of pregnancy loss, and with at least one living child. DNA was extracted from the patient samples, and the relevant genes (FVL, FII, and MTHFR) were amplified by PCR, followed by restriction fragment length polymorphism, to assess the polymorphisms in these genes. The allelic frequencies of polymorphisms were not significantly different between the case and control groups. Polymorphisms in the MTHFR, FVL, and FII genes were not associated with recurrent miscarriage during the first trimester of pregnancy in Brazilian women (P = 0.479; P = 0.491 and P = 0.107, respectively). However, the etiologic identification of genetic factors is important for genetic counseling.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1676-5680 1676-5680
DOI:	10.4238/gmr.15038156