Simultaneous determination of cefepime, vancomycin and imipenem in human plasma of burn patients by high-performance liquid chromatography

A liquid chromatographic method with UV detection for simultaneous determination of cefepime, vancomycin and imipenem has been developed. Cefuroxime was used as internal standard. After the clean up of samples by plasma protein precipitation, 5 μl of the extract were injected into the chromatograph...

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Bibliographic Details
Published in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 860; no. 2; pp. 241 - 245
Main Authors:	López, K.J. Vera, Bertoluci, D. Faria, Vicente, K.M., Dell’Aquilla, A.M., Santos, S.R.C. Jorge
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 15-12-2007 Elsevier Science
Subjects:	Adolescent Analysis Analytical, structural and metabolic biochemistry Anti-Bacterial Agents - blood Biological and medical sciences Burns - blood Calibration Cefepime Cephalosporins - blood Chromatography, High Pressure Liquid - methods Drug Monitoring Female Fundamental and applied biological sciences. Psychology General pharmacology Humans Imipenem Imipenem - blood Liquid–liquid chromatography Male Medical sciences Middle Aged Pharmacology. Drug treatments Reference Standards Reproducibility of Results Sensitivity and Specificity Spectrophotometry, Ultraviolet Vancomycin Vancomycin - blood Vancomycin Cefepime Liquid–liquid chromatography Imipenem Human Biological fluid Chemical analysis Peptides β-Lactams HPLC chromatography Patient Liquid chromatography Blood plasma Burn Cephalosporin derivatives Carbapenem derivatives Antibiotic Liquid-liquid chromatography Glycopeptide Polypeptide Simultaneous measurement Antibacterial agent
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Summary:	A liquid chromatographic method with UV detection for simultaneous determination of cefepime, vancomycin and imipenem has been developed. Cefuroxime was used as internal standard. After the clean up of samples by plasma protein precipitation, 5 μl of the extract were injected into the chromatograph and peaks were eluted from the Sulpelcosil™ LC-18 column using a mobile phase consisting of 0.075 M acetate buffer:acetonitrile (92:8, v/v), pH 5.0 at low rate (0.8 ml/min). The detection wavelength was 230 nm. The limit of detection was 0.4 μg/ml for cefepime and 0.2 μg/ml for vancomycin and imipenem. The method was applied to plasma samples of burn patients, and only small volumes of plasma were required for the simultaneous determination of those antimicrobial agents.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1570-0232 1873-376X
DOI:	10.1016/j.jchromb.2007.10.041