Rapid plant DNA and RNA extraction protocol using a bench drill

Rapid plant DNA and RNA extraction protocol using a bench drill

Plant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after. We developed an optimized protocol for plant DNA and R...

Full description

Saved in:
Bibliographic Details
Published in:Genetics and molecular research Vol. 18; no. 3; p. 1
Main Authors: Ferreira, CF, Gutierrez, DL, Kreuze, JF, Iskra-Caruana, ML, Chabannes, M, Barbosa, ACO, Santos, TA, Silva, AGS, Santos, RMF, Amorim, EP, de Oliveira, SAS, Jesus, ON
Format: Journal Article
Language:English
Published: Ribeirao Preto Fundacao de Pesquisas Cientificas de Ribeirao Preto 2019
Ribeirão Preto foundation for Scientific Research (FUNPEC)
Subjects:
Deoxyribonucleic acid
Developing countries
DNA
Gel electrophoresis
Gene expression
Genetic analysis
Genetic diversity
Laboratories
LDCs
Leaves
Life Sciences
Nucleic acids
Plant extracts
Protocol
Ribonucleic acid
RNA
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Plant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after. We developed an optimized protocol for plant DNA and RNA extraction that uses an inexpensive bench drill and plastic bags and does not require liquid nitrogen. DNA from leaves and RNA from leaves and roots of banana, pineapple, citrus, papaya, passion fruit and cassava, were extracted using a basic cetyltrimethylammonium bromide method. Both nucleic acids were quantified and evaluated for quality based on agarose gel electrophoresis. The DNA and RNA extractions were successful for all species, and RNA quality in pellets was maintained after storage at room temperature for three weeks. This protocol can reduce costs considerably in laboratories with ongoing routine activities of DNA and RNA extraction for genetic diversity and gene expression analyses, where other conventional methods have not been successful due to explant, condition of samples and quantity and quality of nucleic acids. This is especially relevant for many laboratories in developing countries where the cost and availability of liquid nitrogen may be a constraint.
ISSN:1676-5680
1676-5680
DOI:10.4238/gmr18394