Electrochemical DNA biosensor for chronic myelocytic leukemia based on hybrid nanostructure

Electrochemical DNA biosensor for chronic myelocytic leukemia based on hybrid nanostructure

[Display omitted] •An electrochemical biodevice was developed to identify the BCR/ABL fusion gene.•A new interface platform was designed from polypyrrole and hybrid nanostructures.•Clinical samples from patients with chronic myeloid leukemia were investigated in bioassays.•The sensing system showed...

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Bibliographic Details
Published in:Bioelectrochemistry (Amsterdam, Netherlands) Vol. 147; p. 108176
Main Authors: Avelino, Karen Y.P.S., Oliveira, Léony S., Santos, Maryana R., Lucena-Silva, Norma, Andrade, César A.S., Oliveira, Maria D.L.
Format: Journal Article
Language:English
Published: Amsterdam Elsevier B.V 01-10-2022
Elsevier BV
Subjects:
Abl gene
Atomic force microscopy
BCR gene
BCR protein
BCR/ABL fusion gene
Biosensor
Biosensors
Chitosan
Chronic myeloid leukemia
Deoxyribonucleic acid
DNA
Electrochemical impedance spectroscopy
Electrochemistry
Fusion protein
Hybrid nanocomposite
Leukemia
Myeloid leukemia
Nanocomposites
Nanoparticles
Plasmids
Polypyrrole
Polypyrroles
Regression coefficients
Selectivity
Spectroscopy
Zinc oxide
Hybrid nanocomposite
Electrochemistry
Zinc oxide
Biosensor
BCR/ABL fusion gene
Polypyrrole
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Summary:[Display omitted] •An electrochemical biodevice was developed to identify the BCR/ABL fusion gene.•A new interface platform was designed from polypyrrole and hybrid nanostructures.•Clinical samples from patients with chronic myeloid leukemia were investigated in bioassays.•The sensing system showed a limit of detection of 0.52 fM with high selectivity.•The bioanalysis technology contributes to the rapid and early diagnosis of cancer. The present research refers to elaborating a new label-free electrochemical biosensor used to detect the BCR/ABL fusion gene. We used a hybrid nanocomposite composed of chitosan and zinc oxide nanoparticles (Chit-ZnONP) immobilized on a polypyrrole (PPy) film. DNA segments were covalently immobilized, allowing biomolecular recognition. Atomic force microscopy (AFM), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS) were used to evaluate the assembly stages of the biosensor. The biosensor’s analytical performance was investigated using recombinant plasmids containing the target oncogene and clinical samples from patients with chronic myeloid leukemia (CML). A limit of detection (LOD) of 1.34 fM, limit of quantification (LOQ) of 4.08 fM, and sensitivity of 34.03 μA fM−1 cm2 were calculated for the BCR/ABL fusion oncogene. The sensing system exhibited high specificity, selectivity, and reproducibility with a standard deviation (SD) of 4.21%. Additionally, a linear response range was observed between 138.80 aM to 13.88 pM with a regression coefficient of 0.96. Also, the biosensor shows easy operationalization and fast analytical response, contributing to the early cancer diagnosis. The proposed nanostructured device is an alternative for the genetic identification BCR/ABL fusion gene.
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ISSN:1567-5394
1878-562X
DOI:10.1016/j.bioelechem.2022.108176