Identification of putative target genes to manipulate Fe and Zn concentrations in rice grains

Rice is the staple food of half of the world's population; however, it is a poor source of essential micronutrients such as Fe and Zn. Since flag leaves are one of the sources of remobilized metals for developing seeds, the identification of the molecular players that might contribute to the pr...

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Bibliographic Details
Published in:	Journal of plant physiology Vol. 167; no. 17; pp. 1500 - 1506
Main Authors:	Sperotto, Raul A., Boff, Tatiana, Duarte, Guilherme L., Santos, Lívia S., Grusak, Michael A., Fett, Janette P.
Format:	Journal Article
Language:	English
Published:	Munich Elsevier GmbH 15-11-2010 Elsevier
Subjects:	Biological and medical sciences Correlation analyses Flags Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Profiling Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes Genes, Plant - genetics Grains Iron Iron - metabolism Leaves Metal homeostasis Oryza - cytology Oryza - genetics Oryza - growth & development Oryza - metabolism Oryza sativa Plant Leaves - genetics Plant Leaves - metabolism Plant physiology and development Plant Proteins - genetics Plant Proteins - metabolism Rice Seeds Seeds - genetics Seeds - growth & development Seeds - metabolism Zinc Zinc - metabolism VIT ZIP NAS FRO Metal homeostasis IRT Iron Gene expression Zinc NAC NRAMP Correlation analyses NA YSL DMA ICP-OES Rice Monocotyledones Grains Homeostasis Identification Metal Target Oryza Gene Plant physiology Gramineae Correlation analysis Angiospermae Herbaceous plant Spermatophyta
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Summary:	Rice is the staple food of half of the world's population; however, it is a poor source of essential micronutrients such as Fe and Zn. Since flag leaves are one of the sources of remobilized metals for developing seeds, the identification of the molecular players that might contribute to the process of metal transport from flag leaves to the seeds may be useful for biofortification purposes. We analyzed the expression of 25 metal-related genes from rice, including rice homologues for YSLs, NRAMPs, ZIPs, IRT1, VIT1 (coding for known or potential metal transporters), as well as NASs, FROs and NAC5 (involved in metal homeostasis) in flag leaves of eight rice cultivars (showing contrasting levels of seed Fe and Zn) during panicle emergence (R3) and grain filling stage (R5). The expression level of nine of these genes ( OsYSL6, OsYSL8, OsYSL14, OsNRAMP1, OsNRAMP7, OsNRAMP8, OsNAS1, OsFRO1 and OsNAC5) in flag leaves exhibited significant correlations with Fe and/or Zn concentrations in the seeds. In this way, our study has provided a short list of putative target genes to manipulate Fe and Zn concentrations in rice grains.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0176-1617 1618-1328
DOI:	10.1016/j.jplph.2010.05.003