Potassium Channel Activation Is Involved in the Cardiovascular Effects Induced by Freeze Dried Syzygium jambolanum (Lam.) DC Fruit Juice

This work aimed to explore the cardiovascular effects induced by freeze-dried juice from Syzygium jambolanum (Lam.) DC fruits (JSJ). JSJ presented high polyphenol content and steroids. HPLC analysis revealed that 2,5-dihydroxybenzoic and caffeic acid were present in higher amounts in the JSJ extract...

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Published in:	BioMed research international Vol. 2018; no. 2018; pp. 1 - 12
Main Authors:	Santos, Carlos A. G., Cordeiro, Angela M. T. M., Araújo, Demetrius A. M., Ribeiro, Thais P., Medeiros, Isac Almeida de, de Vasconcelos, Aliny P., Assis, Valéria L., da Silva, Tays A. F., Machado Calzerra, Natália T., Maciel, Priscilla M. P., de Azevedo, Fátima de L. A. A., Araújo, Islania G. A., Assis, Kívia S., Meireles, Bruno R. L. A.
Format:	Journal Article
Language:	English
Published:	Cairo, Egypt Hindawi Publishing Corporation 01-01-2018 Hindawi John Wiley & Sons, Inc Hindawi Limited
Subjects:	Acids Activation Animals Antioxidants Arteries Attenuation Barium chloride Beverages Blood vessels Caffeic acid Cardiovascular disease Cardiovascular system Channels Depolarization Dietary minerals Dilatation Endothelium Flavonoids Freeze Drying Fruit and Vegetable Juices Fruit juices Fruits Glibenclamide High performance liquid chromatography Hypotension Hypotension - chemically induced Hypotension - metabolism Hypotension - physiopathology Laboratory animals Liquid chromatography Medical research Mesenteric Arteries - metabolism Mesenteric Arteries - physiopathology Metabolites Nutrition Pharmacology Phenylephrine Phytochemicals Potassium Potassium Channel Blockers - chemistry Potassium Channel Blockers - pharmacology Potassium channels Potassium Channels - metabolism Potassium chloride Rats Rats, Wistar Rodents Smooth muscle Steroid hormones Steroids Systematic review Syzygium Syzygium - chemistry Tetraethylammonium Vasodilation Vasodilation - drug effects Veins & arteries Brazil Australia
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Summary:	This work aimed to explore the cardiovascular effects induced by freeze-dried juice from Syzygium jambolanum (Lam.) DC fruits (JSJ). JSJ presented high polyphenol content and steroids. HPLC analysis revealed that 2,5-dihydroxybenzoic and caffeic acid were present in higher amounts in the JSJ extract. In rat, JSJ induces hypotension and vasodilatation in mesenteric arteries, with or without vascular endothelium. JSJ-mediated vasodilation response against contractions induced with KCl (60 mM) depolarizing solution was significantly lower than the responses induced by JSJ when evaluated against phenylephrine-induced contractions. To investigate the involvement of potassium channels we used Tyrode’s solution with KCl (20 mM) or tetraethylammonium (1.0, 3.0, or 5.0 mM). In these conditions JSJ-induced effects were significantly attenuated. To investigate the potassium channel subtypes involved in the response, we used 4-aminopyridine, glibenclamide, BaCl2, and iberiotoxin. In the presence (simultaneous) of different potassium channel blockers we observed a significant attenuation of JSJ-induced effect. Inhibition was also observed when using BaCl2, glibenclamide, or 4-aminopyridine, separately. However, incubation with iberiotoxin did not promote changes in either maximum effect, or potency. We also evidenced a discrete participation of CaV channels in the JSJ-induced vasorelaxant effect. In addition, patch-clamp studies demonstrated that JSJ could activate potassium channels. In conclusion, JSJ promotes hypotension and vasorelaxation in rats, involving, at least, the activation of potassium channels.
Bibliography:	Academic Editor: Mauro S. Oliveira
ISSN:	2314-6133 2314-6141
DOI:	10.1155/2018/4827461