Immunohistochemical studies in acute and chronic canine chagasic cardiomyopathy

A major characteristic of Chagas' disease is a myocarditis constituted primarily of mononuclear cells, both during the acute and chronic phases of the disease. Using monoclonal antibodies and image analyses we have quantified canine CD8(+) T cells (caCD8(+) T cells), canine CD4(+) T cells (caCD...

Full description

Saved in:

Bibliographic Details
Published in:	Virchows Archiv : an international journal of pathology Vol. 441; no. 1; pp. 69 - 76
Main Authors:	Caliari, Marcelo V, de Lana, Marta, Cajá, Rosângela A F, Carneiro, Cláudia M, Bahia, Maria T, Santos, César A B, Magalhães, Gustavo A, Sampaio, Ivan B M, Tafuri, Washington L
Format:	Journal Article
Language:	English
Published:	Germany 01-07-2002
Subjects:	Acute Disease Animals CD4-Positive T-Lymphocytes - pathology CD8-Positive T-Lymphocytes - pathology Chagas Cardiomyopathy - metabolism Chagas Cardiomyopathy - pathology Chronic Disease Dogs Humans Immunohistochemistry Immunophenotyping Lymphocyte Count Neutrophils - pathology
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	A major characteristic of Chagas' disease is a myocarditis constituted primarily of mononuclear cells, both during the acute and chronic phases of the disease. Using monoclonal antibodies and image analyses we have quantified canine CD8(+) T cells (caCD8(+) T cells), canine CD4(+) T cells (caCD4(+) T cells) and neutrophils in canine chagasic myocardiopathy induced by two strains isolated from the first human clinical case of Chagas' disease. We also evaluated the influence of tissue parasitism in the genesis of chronic myocarditis through immunohistochemistry. As in human myocarditis, there was a predominance of T lymphocytes in the inflammatory infiltrate in all animals studied. In the dogs inoculated with strain Berenice 78 (Be78) and necropsied during the acute phase of infection, we found 58% caCD8(+) and 42% caCD4(+) T cells. In chronically infected animals, 53% of T cells were represented by caCD8(+) and 47% were caCD4(+) T cells. Since normal canine lymphoid organs are constituted by 70-80% caCD4(+) T cells and 20-30% caCD8(+) T cells our results indicate a higher proliferation of caCD8(+) T cells in dogs inoculated with the Be78 strain. In chronic myocarditis induced by the Berenice 62 (Be62) strain, caCD8(+) cells constituted 33% of the T cells and 67% were caCD4(+) T cells, a proportion similar to that found in normal canine lymphoid organs. Since the Be78 strain induces greater loss of myocardiocytes than strain Be62, we believe that the caCD8(+) T cells, among other factors, can be important in the genesis of these lesions. Amastigote nests and immunohistochemically labelled Trypanosoma cruzi antigen were not found in dogs necropsied during the chronic phase. The absence of the parasite in the myocardium suggests the involvement of other mechanisms in the genesis of the inflammatory process.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0945-6317 1432-2307
DOI:	10.1007/s00428-001-0542-4