Identification of a new nucleopolyhedrovirus from naturally-infected Condylorrhiza vestigialis (Guenée) (Lepidoptera: Crambidae) larvae on poplar plantations in South Brazil

A baculovirus was isolated from larvae of Condylorrhiza vestigialis (Guenée) (Lepidoptera: Crambidae), a pest of a forest species known as Poplar (family Salicaceae, genus: Populus) with high economic value. Electron microscopy analysis of the occlusion body obtained from diseased larvae showed poly...

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Bibliographic Details
Published in:	Journal of invertebrate pathology Vol. 102; no. 2; pp. 149 - 154
Main Authors:	Castro, Maria Elita B., Ribeiro, Zilda Maria A., Santos, Ana Cláudia B., Souza, Marlinda L., Machado, Edilene B., Sousa, Nilton J., Moscardi, Flávio
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier Inc 01-10-2009 Elsevier
Subjects:	Animals Baculovirus Biological and medical sciences Biological control Cell Line Characterization Condylorrhiza vestigialis Condylorrhiza vestigialis multiple nucleopolyhedrovirus Control CoveMNPV Crambidae DNA, Viral - analysis forest plantations Fundamental and applied biological sciences. Psychology Genes, Viral in vitro infection infection Insect Control insect viruses Insecta Invertebrates Larva - ultrastructure Larva - virology larvae Lepidoptera Moths - ultrastructure Moths - virology natural infection Nucleopolyhedrovirus Nucleopolyhedrovirus - genetics Nucleopolyhedrovirus - isolation & purification Nucleopolyhedrovirus - pathogenicity Nucleopolyhedrovirus - ultrastructure occlusion bodies pathogen identification pathogenicity Pathology Pest Control, Biological Phytopathology. Animal pests. Plant and forest protection Plant Diseases - parasitology polyhedra Polyhedrin Populus Populus sp Protozoa. Invertebrates restriction mapping Salicaceae Spodoptera frugiperda Taxonomic identification taxonomy Viral DNA analysis viral morphology viral proteins Viral Structural Proteins - analysis Viral Structural Proteins - genetics Brazil South America America Nucleopolyhedrovirus Characterization Viral DNA analysis CoveMNPV Condylorrhiza vestigialis Populus sp Polyhedrin Taxonomic identification in vitro infection Baculovirus Entomopathogen Taxonomy Insecta Biological control Crambidae First record Pest Microbiological control Baculoviridae Dicotyledones Angiospermae Hardwood forest tree Populus In vitro Salicaceae Virus Infection Larva Arthropoda DNA Lepidoptera Spermatophyta Invertebrata
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Summary:	A baculovirus was isolated from larvae of Condylorrhiza vestigialis (Guenée) (Lepidoptera: Crambidae), a pest of a forest species known as Poplar (family Salicaceae, genus: Populus) with high economic value. Electron microscopy analysis of the occlusion body obtained from diseased larvae showed polyhedra containing multiple nucleocapsids per envelope. This baculovirus was thus named Condylorrhiza vestigialis multiple nucleopolyhedrovirus (CoveMNPV) and characterized by its DNA restriction endonuclease pattern, polyhedral protein, viral protein synthesis, and infectivity in insect cell lines. Restriction endonuclease profiles of viral DNA digested with five restriction enzymes were obtained and the CoveMNPV genome size was estimated to be 81 ± 2.5 kbp. The isolation of the polyhedra (OBs) was done from the crude extract of infected larvae by ultracentrifugation through sucrose gradients. These viral particles were analyzed by denaturing polyacrylamide gel electrophoresis (SDS–PAGE), which showed a strong band with approximately 33 kDa, corresponding to the main protein of the occlusion bodies (polyhedrin). Also, a similar band was observed for CoveMNPV infected Spodoptera frugiperda cells (SF-21 AE) pulse-labeled with [ 35S] methionine and fractionated by SDS–PAGE. Of the four insect cell lines tested for susceptibility to CoveMNPV infection, the SF-21 AE was the most susceptible with occlusion bodies produced in most of the inoculated cells. This is the first record of an NPV from C. vestigialis.
Bibliography:	http://dx.doi.org/10.1016/j.jip.2009.07.011 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-2011 1096-0805
DOI:	10.1016/j.jip.2009.07.011