A chronic high-fat diet causes sperm head alterations in C57BL/6J mice

A chronic high-fat diet causes sperm head alterations in C57BL/6J mice

A chronic-positive energetic balance has been directly correlated with infertility in men, but the involved mechanisms remain unknown. Herein we investigated weather in a mouse model a chronic feeding with a diet supplemented with chicken fat affects sperm head morphology. To accomplish this, we fed...

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Bibliographic Details
Published in:Heliyon Vol. 5; no. 11; p. e02868
Main Authors: Funes, A., Saez Lancellotti, T.E., Santillan, L.D., Della Vedova, M.C., Monclus, M.A., Cabrillana, M.E., Gomez Mejiba, S.E., Ramirez, D.C., Fornes, M.W.
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01-11-2019
Elsevier
Subjects:
Cell biology
Diet
Dietary fat
Immunology
Inflammation
Male fertility
Molecular biology
Mouse model
Oxidative stress
Proteins
Sperm-head morphology
Proteins
Oxidative stress
Immunology
Diet
Male fertility
Cell biology
Dietary fat
Mouse model
Inflammation
Sperm-head morphology
Molecular biology
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Summary:A chronic-positive energetic balance has been directly correlated with infertility in men, but the involved mechanisms remain unknown. Herein we investigated weather in a mouse model a chronic feeding with a diet supplemented with chicken fat affects sperm head morphology. To accomplish this, we fed mice for 16 weeks with either control food (low-fat diet, LFD) or control food supplemented with 22% chicken fat (high-fat diet, HFD). At the end of the feeding regimen, we measured: redox and inflammatory changes, cholesterol accumulation in testis and analyzed testicular morphological structure and ultra-structure and liver morphology. We found that the mice fed HFD resembled some features of the human metabolic syndrome, including systemic oxidative stress and inflammation, this group showed an increment in the following parameters; central adiposity (adiposity index: 1.07 ± 0.10 vs 2.26 ± 0.17), dyslipidemia (total cholesterol: 153.3 ± 2.6 vs 175.1 ± 8.08 mg/dL), insulin resistance (indirect Insulin resistance index, TG/HDL-c: 2.94 ± 0.33 vs 3.68 ± 0.15) and fatty liver. Increased cholesterol content measured by filipin was found in the testicles from HFD (fluorescence intensity increase to 50%), as well as an alteration of spermiogenesis. Most remarkably, a disorganized manchette-perinuclear ring complex and an altered morphology of the sperm head were observed in the spermatozoa of HFD-fed mice. These results add new information to our understanding about the mechanisms by which systemic oxidative stress and inflammation may influence sperm-head morphology and indirectly male fertility. Cell biology; Immunology; Molecular biology; Proteins; Diet; Male fertility; Mouse model; Inflammation; Sperm-head morphology; Dietary fat; Oxidative stress
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ISSN:2405-8440
2405-8440
DOI:10.1016/j.heliyon.2019.e02868