Characterization of Burkholderia pseudomallei from spontaneous melioidosis in a Bornean orangutan

Characterization of Burkholderia pseudomallei from spontaneous melioidosis in a Bornean orangutan

Melioidosis is a potentially fatal disease affecting humans and a wide range of animal species; it is often underdiagnosed and underreported in veterinary medicine in Indonesia. This study aimed to characterize morphological and molecular features of , the causative agent of melioidosis which caused...

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Bibliographic Details
Published in:Veterinary World Vol. 13; no. 11; pp. 2459 - 2468
Main Authors: Testamenti, Vincentius Arca, Surya, Maryati, Saepuloh, Uus, Iskandriati, Diah, Tandang, Maryos Vigouri, Kristina, Lia, Wahyudi, Aris Tri, Sajuthi, Dondin, Santi, Vivi Dwi, Patispathika, Fiet Hayu, Wahyu, Muhtadin, Nurcahyo, Anton, Pamungkas, Joko
Format: Journal Article
Language:English
Published: India Veterinary World 01-11-2020
Subjects:
Agar
Agglutination
Bacteria
Burkholderia pseudomallei
Chemotaxis
Flagella
Genetic diversity
Genetic relationship
Genotypes
Genotyping
Latex agglutination
Melioidosis
molecular characterization
Morphology
Multilocus sequence typing
nonhuman primate
orangutan
Physical characteristics
Polymerase chain reaction
Veterinary medicine
Thailand
Malaysia
Indonesia
Burkholderia pseudomallei
melioidosis
molecular characterization
nonhuman primate
orangutan
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Summary:Melioidosis is a potentially fatal disease affecting humans and a wide range of animal species; it is often underdiagnosed and underreported in veterinary medicine in Indonesia. This study aimed to characterize morphological and molecular features of , the causative agent of melioidosis which caused the death of a Bornean orangutan. Pulmonary abscess samples were cultured on several types of media, including Ashdown agar, Ashdown broth, and MacConkey agar. Type three secretion system 2 real-time polymerase chain reaction (PCR) and latex agglutination tests were performed to identify the bacteria. Morphological characteristics were compared to all previously published morphotypes. Subsequently, the bacteria were characterized by multilocus sequence typing (MLST) and -like flagellum/ -like flagellum and chemotaxis PCR. The results of the genotyping were afterward compared to all genotypes from Southeast Asia. Multiple morphotypes of were perceived during the growth on Ashdown agar. Furthermore, it was identified by MLST that the Type I and Type II morphotypes observed in this study were clones of a single ST, ST54, which is predominantly found in humans and the environment in Malaysia and Thailand, although a very limited number of reports was published in association with animals. Moreover, the E-BURST analysis showed that the ST is grouped together with isolates from Southeast Asian countries, including Malaysia, Thailand, Singapore, and Cambodia. ST54 was predicted to be the founding genotype of several STs from those regions. ST54 that caused the death of a Bornean orangutan has a distant genetic relationship with other STs which were previously reported in Indonesia, implying a vast genetic diversity in Indonesia that has not been discovered yet.
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ISSN:0972-8988
2231-0916
DOI:10.14202/vetworld.2020.2459-2468