Polymerase Chain Reaction-Restriction Fragment Length Polymorphism of Mitochondrial 12S rRNA Gene: A Simple Method for Identification of Poultry Meat Species

Polymerase Chain Reaction-Restriction Fragment Length Polymorphism of Mitochondrial 12S rRNA Gene: A Simple Method for Identification of Poultry Meat Species

Chicken (Gallus gallus), duck (Anas platyrhynchos), turkey (Meleagris gallopavo), guinea fowl (Numida meleagris) and quail (Coturnix japonica) are the common poultry species consumed as meat throughout the world. In this work, a molecular technique has been developed for identification and different...

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Bibliographic Details
Published in:Veterinary research communications Vol. 31; no. 4; pp. 447 - 455
Main Authors: Girish, P. S, Anjaneyulu, A. S. R, Viswas, K. N, Santhosh, F. H, Bhilegaonkar, K. N, Agarwal, R. K, Kondaiah, N, Nagappa, K
Format: Journal Article
Language:English
Published: Netherlands Dordrecht : Kluwer Academic Publishers 01-05-2007
Springer Nature B.V
Subjects:
Anas platyrhynchos
Animals
Chickens - genetics
Coturnix
Coturnix japonica
Ducks - genetics
Gallus gallus
genes
Meat - classification
Meleagris gallopavo
Mitochondria, Muscle - genetics
mitochondrial DNA
new methods
Numida meleagris
polymerase chain reaction
Polymerase Chain Reaction - veterinary
Polymorphism, Restriction Fragment Length
poultry
poultry meat
product authenticity
Quail - genetics
quantitative analysis
restriction fragment length polymorphism
ribosomal RNA
RNA, Ribosomal - genetics
species differences
Species Specificity
Turkeys - genetics
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Summary:Chicken (Gallus gallus), duck (Anas platyrhynchos), turkey (Meleagris gallopavo), guinea fowl (Numida meleagris) and quail (Coturnix japonica) are the common poultry species consumed as meat throughout the world. In this work, a molecular technique has been developed for identification and differentiation of meat originating from these species. This tool helps in detection of misrepresentation of different poultry meats. The technique involves the extraction of DNA from the given sample, polymerase chain reaction (PCR) amplification of mitochondrial 12S rRNA gene using universal primers, restriction analysis with selected restriction enzymes, followed by identification of meat species based on restriction fragment length polymorphism (RFLP) pattern. In this study, we used HinfI, Mph1103I, MvaI, and Eco47I to identify and differentiate to poultry species referred to above. This species identification technique has also been applied successfully to processed meat products including those cooked at 120[composite function (small circle)]C for 30 min. Simplicity of interpretation of results combined with versatility makes this a convenient and appropriate technique in the hands of meat analysts for identifying poultry meat species.
Bibliography:http://dx.doi.org/10.1007/s11259-006-3390-5
ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0165-7380
1573-7446
DOI:10.1007/s11259-006-3390-5