Neutralising ability of Terminalia fagifolia extract (Combretaceae) against the in vitro neuromuscular effects of Bothrops jararacussu venom

The ability of Terminalia fagifolia hydroalcoholic extract (Tf-HE) to neutralise the paralysis and myotoxicity induced by Bothrops jararacussu venom was assayed using mouse phrenic nerve-diaphragm (PND) preparation and two varieties of chick biventer cervicis (BC) preparations. Tf-HE 100 μg/mL and 5...

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Bibliographic Details
Published in:	Natural product research Vol. 31; no. 23; pp. 2783 - 2787
Main Authors:	Tribuiani, Natália, Tavares, Marylu Oliveira, Santana, Monique Neves, Fontana Oliveira, Isadora Caruso, Amaral Filho, Jorge do, Silva, Magali Glauzer, dos Santos, Marcio Galdino, Cogo, José Carlos, Floriano, Rafael Stuani, Cogo-Müller, Karina, Oshima-Franco, Yoko
Format:	Journal Article
Language:	English
Published:	England Taylor & Francis 02-12-2017 Taylor & Francis Ltd
Subjects:	Acetylcholine Animals Bothrops Bothrops jararacussu Chickens Creatine Creatine kinase Creatine Kinase - metabolism Crotalid Venoms - toxicity Diaphragm Diaphragm - drug effects Drug Evaluation, Preclinical - methods Incubation Male Medicinal plant Mice Myotoxicity Neuromuscular Agents - toxicity Neuromuscular junctions Neurotoxicity Paralysis Phrenic nerve Phrenic Nerve - drug effects Plant extracts Plant Extracts - pharmacology Snake venom Terminalia - chemistry Terminalia fagifolia Toxicity Venom Neurotoxicity Snake venom Myotoxicity Medicinal plant
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Summary:	The ability of Terminalia fagifolia hydroalcoholic extract (Tf-HE) to neutralise the paralysis and myotoxicity induced by Bothrops jararacussu venom was assayed using mouse phrenic nerve-diaphragm (PND) preparation and two varieties of chick biventer cervicis (BC) preparations. Tf-HE 100 μg/mL and 500 μg/mL were tested against 40 and 200 μg of venom/mL in PND and BC preparations, respectively, using pre- and post-venom incubation treatments. The effects of Tf-HE against the myotoxicity caused by venom were evaluated via histological analysis (PND) and creatine kinase (CK) release (BC). Tf-HE was able to reverse the venom paralysis in both preparation types. The contractures to exogenous ACh in BC preparations showed that Tf-HE may act on extrinsic, preserving those intrinsic postsynaptic receptors. There was a positive correlation between CK and morphological changes. The high non-hemolytic saponin content can explain the Tf-HE efficacy against the toxic effects of B. jararacussu venom in vertebrate neuromuscular preparations.
ISSN:	1478-6419 1478-6427
DOI:	10.1080/14786419.2017.1292506