Search Results - "Sanson, Kendall R."

Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities by Sanson, Kendall R., Hanna, Ruth E., Hegde, Mudra, Donovan, Katherine F., Strand, Christine, Sullender, Meagan E., Vaimberg, Emma W., Goodale, Amy, Root, David E., Piccioni, Federica, Doench, John G.

“…The creation of genome-wide libraries for CRISPR knockout (CRISPRko), interference (CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation…”
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Genetic screens in isogenic mammalian cell lines without single cell cloning by DeWeirdt, Peter C., Sangree, Annabel K., Hanna, Ruth E., Sanson, Kendall R., Hegde, Mudra, Strand, Christine, Persky, Nicole S., Doench, John G.

“…Isogenic pairs of cell lines, which differ by a single genetic modification, are powerful tools for understanding gene function. Generating such pairs of…”
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Optimization of AsCas12a for combinatorial genetic screens in human cells by DeWeirdt, Peter C., Sanson, Kendall R., Sangree, Annabel K., Hegde, Mudra, Hanna, Ruth E., Feeley, Marissa N., Griffith, Audrey L., Teng, Teng, Borys, Samantha M., Strand, Christine, Joung, J. Keith, Kleinstiver, Benjamin P., Pan, Xuewen, Huang, Alan, Doench, John G.

“…Cas12a RNA-guided endonucleases are promising tools for multiplexed genetic perturbations because they can process multiple guide RNAs expressed as a single…”
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Massively parallel assessment of human variants with base editor screens by Hanna, Ruth E., Hegde, Mudra, Fagre, Christian R., DeWeirdt, Peter C., Sangree, Annabel K., Szegletes, Zsofia, Griffith, Audrey, Feeley, Marissa N., Sanson, Kendall R., Baidi, Yossef, Koblan, Luke W., Liu, David R., Neal, James T., Doench, John G.

“…Understanding the functional consequences of single-nucleotide variants is critical to uncovering the genetic underpinnings of diseases, but technologies to…”
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Orthologous CRISPR–Cas9 enzymes for combinatorial genetic screens by Najm, Fadi J, Strand, Christine, Donovan, Katherine F, Hegde, Mudra, Sanson, Kendall R, Vaimberg, Emma W, Sullender, Meagan E, Hartenian, Ella, Kalani, Zohra, Fusi, Nicolo, Listgarten, Jennifer, Younger, Scott T, Bernstein, Bradley E, Root, David E, Doench, John G

“…Combinatorial CRISPR screens are improved by using Cas9 enzymes from two different organisms. Combinatorial genetic screening using CRISPR–Cas9 is a useful…”
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