Intergenotypic chimeric hepatitis E viruses (HEVs) with the genotype 4 human HEV capsid gene in the backbone of genotype 3 swine HEV are infectious in pigs

Intergenotypic chimeric hepatitis E viruses (HEVs) with the genotype 4 human HEV capsid gene in the backbone of genotype 3 swine HEV are infectious in pigs

Genotypes 1 and 2 hepatitis E virus (HEV) infect only humans whereas genotypes 3 and 4 HEV infect both humans and pigs. To evaluate the mechanism of cross-species HEV infection between humans and swine, in this study we constructed five intergenotypic chimeric viruses and tested for their infectivit...

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Bibliographic Details
Published in:Virus research Vol. 156; no. 1-2; pp. 141 - 146
Main Authors: Feagins, Alicia R., Córdoba, Laura, Sanford, Brent J., Dryman, Barbara A., Huang, Yao-Wei, LeRoith, Tanya, Emerson, Suzanne U., Meng, Xiang-Jin
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01-03-2011
Subjects:
Animals
capsid
Capsid Proteins - genetics
Cell Line, Tumor
Chimeric viruses
chimerism
Cross-species infection
Gene Order
genes
Genotype
Hep G2 Cells
hepatitis E
Hepatitis E - veterinary
Hepatitis E - virology
Hepatitis E virus
Hepatitis E virus (HEV)
Hepatitis E virus - genetics
Hepatitis E virus - metabolism
Hepatitis E virus - pathogenicity
Host range
Humans
Immunoglobulin G - blood
pathogenicity
Pigs
Swine
Swine Diseases - virology
Viremia
Virus Shedding
viruses
Pigs
Host range
Cross-species infection
Hepatitis E virus (HEV)
Chimeric viruses
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Summary:Genotypes 1 and 2 hepatitis E virus (HEV) infect only humans whereas genotypes 3 and 4 HEV infect both humans and pigs. To evaluate the mechanism of cross-species HEV infection between humans and swine, in this study we constructed five intergenotypic chimeric viruses and tested for their infectivity in vitro and in pigs. We demonstrated that chimeric viruses containing the ORF2 capsid gene either alone or in combination with its adjacent 5′ junction region (JR) and 3′ noncoding region (NCR) from a genotype 4 human HEV in the backbone of a genotype 3 swine HEV are replication-competent in Huh7 cells and infectious in HepG2/C3A cells and in pigs, and thus supporting the hypothesis that genotypes 3 and 4 human HEV are of swine origin. However, chimeric viruses containing the JR+ORF2+3′ NCR of genotypes 3 or 4 HEV in the backbone of genotype 1 human HEV failed to infect pigs, suggesting that other genomic regions such as 5′ NCR and ORF1 may also be involved in HEV cross-species infection. The results from this study provide the first experimental evidence of the exchangeability of the capsid gene between genotype 3 swine HEV and genotype 4 human HEV, and have important implications for understanding the mechanism of HEV cross-species infection.
Bibliography:http://dx.doi.org/10.1016/j.virusres.2010.12.011
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ISSN:0168-1702
1872-7492
DOI:10.1016/j.virusres.2010.12.011