Detection of the Amphibian Pathogens Chytrid Fungus (Batrachochytrium dendrobatidis) and Ranavirus in West Texas, USA, Using Environmental DNA

Detection of the Amphibian Pathogens Chytrid Fungus (Batrachochytrium dendrobatidis) and Ranavirus in West Texas, USA, Using Environmental DNA

Environmental DNA (eDNA) methods provide novel options for the detection of pathogens. The amphibian pathogens Batrachochytrium dendrobatidis (Bd) and Ranavirus have been relatively understudied in Texas, US, so we applied eDNA assays for the surveillance of these pathogens in the upper Brazos River...

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Bibliographic Details
Published in:Journal of wildlife diseases Vol. 56; no. 3; pp. 702 - 706
Main Authors: Barnes, Matthew A., Brown, Audrey D., Daum, Mikaela N., de la Garza, Karla A., Driskill, Jackson, Garrett, Kylie, Goldstein, Madeleine S., Luk, Alaia, Maguire, Joel I., Moke, Robert, Ostermaier, Emily M., Sanders, Yorick M., Sandhu, Theodore, Stith, Aryiah, Suresh, Varshini V.
Format: Journal Article
Language:English
Published: United States Wildlife Disease Association 01-07-2020
Subjects:
Amphibian disease
Amphibians
Animals
Batrachochytrium - isolation & purification
chytrid fungus
DNA Virus Infections - epidemiology
DNA Virus Infections - veterinary
DNA Virus Infections - virology
eDNA
Environmental DNA - genetics
Environmental DNA - isolation & purification
Mycoses - epidemiology
Mycoses - microbiology
Mycoses - veterinary
Ranavirus
Ranavirus - isolation & purification
Rivers
SHORT COMMUNICATIONS
Texas
west Texas
Texas
Bd
Ranavirus
chytrid fungus
eDNA
Amphibian disease
west Texas
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Summary:Environmental DNA (eDNA) methods provide novel options for the detection of pathogens. The amphibian pathogens Batrachochytrium dendrobatidis (Bd) and Ranavirus have been relatively understudied in Texas, US, so we applied eDNA assays for the surveillance of these pathogens in the upper Brazos River basin near the Texas panhandle. We collected water samples from five urban playa lakes and one reservoir in and around Lubbock, Texas. Quantitative PCR detected both Bd and Ranavirus at one playa lake, representing novel detection of both pathogens in the region. Based on these results, we recommend increased monitoring for the pathogens and symptoms of amphibian disease throughout the region.
ISSN:0090-3558
1943-3700
DOI:10.7589/2019-08-212