Targeting of cationic liposomes to skin-associated bacteria

Targeting of cationic liposomes to skin-associated bacteria

Cationic vesicles were produced by incorporating positively-charged stearylamine into the lipid bilayers. The addition of stearylamine has been shown to facilitate targeting of liposomes to the skin-associated bacterium Staphylococcus epidermis. The adsorption of the liposomes to films of the bacter...

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Bibliographic Details
Published in:Pesticide Science Vol. 46; no. 3; pp. 255 - 261
Main Authors: Sanderson, N.M, Jones, M.N
Format: Journal Article Conference Proceeding
Language:English
Published: London John Wiley & Sons, Ltd 01-03-1996
Wiley
London :John Wiley & Sons Ltd
Subjects:
adsorption
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
bacteria
biofilm
Biological and medical sciences
cations
drug delivery systems
infection
Langmuir isotherm
liposomes
Medical sciences
Pharmacology. Drug treatments
Staphylococcus epidermidis
staphylococcusepidermidis
strength
Visicles
Cationic complex
Vesicle
Targeting
Liposome
Staphylococcus epidermidis
Adsorption
Ionic strength
Biofilm
Dosage form
Bacteria
Micrococcales
Micrococcaceae
Antibacterial agent
Physicochemical properties
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Summary:Cationic vesicles were produced by incorporating positively-charged stearylamine into the lipid bilayers. The addition of stearylamine has been shown to facilitate targeting of liposomes to the skin-associated bacterium Staphylococcus epidermis. The adsorption of the liposomes to films of the bacteria immobilized on a solid surface has been studied. The extent of adsorption as a function of liposomal lipid concentration could be described in terms of a Langmuir adsorption isotherm, applicable to situations in which species are adsorbed as monolayers on solid surfaces. Analysis on the basis of the Langmuir model enabled the determination of the maximum theoretical targeting to the bacteria and association/dissociation constants for the interaction. The adsorption was examined under conditions of varying ionic strength. Increasing the ionic strength had the effect of decreasing the extent of targeting and suggested that the interaction between the cationic vesicles and sites on the bacterial surface was mediated by electrical double-layer effects. It follows that electrostatic effects make a major contribution to the interaction between these vesicles and S. epidermidis. The attachment of the vesicles is reversible and ionic strength-induced removal of vesicles from the bacteria could be used as a tool to study the delivery of liposomally encapsulated bactericide to the biofilm.
Bibliography:istex:740FC43CBE4352D379F6C5CD76161E91CDEB8F61
ark:/67375/WNG-0G5M59VR-8
ArticleID:PS345
ISSN:0031-613X
1526-498X
1096-9063
DOI:10.1002/(SICI)1096-9063(199603)46:3<255::AID-PS345>3.0.CO;2-Y