The different response of Brassica napus genotypes to microspore embryogenesis induced by heat shock and trichostatin A is not determined by changes in cell wall structure and composition but by different stress tolerance

During microspore embryogenesis, microspores are induced to develop into haploid embryos. In Brassica napus, microspore embryogenesis is induced by a heat shock (HS), which initially produces embryogenic structures with different cell wall architectures and compositions, and with different potential...

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Bibliographic Details
Published in:	Physiologia plantarum Vol. 176; no. 3; pp. e14405 - n/a
Main Authors:	Camacho‐Fernández, Carolina, Corral‐Martínez, Patricia, Calabuig‐Serna, Antonio, Arjona‐Mudarra, Paloma, Sancho‐Oviedo, Daniel, Boutilier, Kim, Seguí‐Simarro, Jose M.
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Publishing Ltd 01-05-2024 Wiley Subscription Services, Inc
Subjects:	Arabinogalactan Brassica Brassica napus Brassica napus - drug effects Brassica napus - genetics Brassica napus - physiology Cell adhesion Cell Wall - drug effects Cell Wall - metabolism Cell walls Cellulose Cellulose esters Composition Embryogenesis Embryonic growth stage Embryos Esterification Genotype Genotypes Heat shock Heat-Shock Response - drug effects Heat-Shock Response - genetics Histone deacetylase Hydroxamic Acids - pharmacology Hypersensitivity Microspores Pectin Pollen - drug effects Pollen - genetics Proteins Rape plants Stress, Physiological Trichostatin A
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Summary:	During microspore embryogenesis, microspores are induced to develop into haploid embryos. In Brassica napus, microspore embryogenesis is induced by a heat shock (HS), which initially produces embryogenic structures with different cell wall architectures and compositions, and with different potentials to develop into embryos. The B. napus DH4079 and DH12075 genotypes have high and very low embryo yields, respectively. In DH12075, embryo yield is greatly increased by combining HS and the histone deacetylase (HDAC) inhibitor trichostatin A (TSA). However, we show that HS + TSA inhibits embryogenesis in the highly embryogenic DH4079 line. To ascertain why TSA has such different effects in these lines, we treated DH4079 and DH12075 microspore cultures with TSA and compared the cell wall structure and composition of the different embryogenic structures in both lines, specifically the in situ levels and distribution of callose, cellulose, arabinogalactan proteins and high and low methyl‐esterified pectin. For both lines, HS + TSA led to the formation of cell walls unfavorable for embryogenesis progression, with reduced levels of arabinogalactan proteins, reduced cell adhesion of inner walls and altered pectin composition. Thus, TSA effects on cell walls cannot explain their different embryogenic response to TSA. We also applied TSA to DH4079 cultures at different times and concentrations before HS application, with no negative effects on embryogenic induction. These results indicate that DH4079 microspores are hypersensitive to combined TSA and HS treatments, and open up new hypotheses about the causes of such hypersensitivity.
Bibliography:	These authors have contributed equally ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0031-9317 1399-3054 1399-3054
DOI:	10.1111/ppl.14405