The genes encoding mammalian chaperonin 60 and chaperonin 10 are linked head-to-head and share a bidirectional promoter

Chaperonins are a class of stress-inducible molecular chaperones involved in protein folding. We report the cloning, sequencing and characterisation of the rat mitochondrial chaperonin 60 and chaperonin 10 genes. The two genes are arranged in a head-to-head configuration and together comprise 14 kb...

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Bibliographic Details
Published in:	Gene Vol. 196; no. 1; pp. 9 - 17
Main Authors:	Ryan, Michael T., Herd, Susanna M., Sberna, Gian, Samuel, Melanie M., Hoogenraad, Nicholas J., Høj, Peter B.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-09-1997
Subjects:	Animals Base Sequence Chaperonin 10 - genetics Chaperonin 60 - genetics Cloning, Molecular Gene Expression Regulation Genome Heat shock Hsp10 Hsp60 Mammals - genetics Molecular Sequence Data Promoter Regions, Genetic Rats Sequence Homology, Nucleic Acid Stress protein tsp, transcription start point oligo, oligodeoxyribonucleotide Stress protein luc, Luc-encoding gene Hsp10 kb, kilobase(s) or 1000 bp N, A/C/G/T bp, base pair(s) HSF, heat-shock factor nt, nucleotide Cpn10, chaperonin 10 UTR, untranslated region Cpn, Cpn-encoding gene Hsp, heat-shock protein Hsp60 CAT, chloramphenicol acetyltransferase ELISA, enzyme-linked imunosorbent assay Luc, firefly luciferase HSE, heat-shock element mt, mitochondrial cat, gene encoding CAT Cpn60, chaperonin 60 cpm, counts per minute Heat shock
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Summary:	Chaperonins are a class of stress-inducible molecular chaperones involved in protein folding. We report the cloning, sequencing and characterisation of the rat mitochondrial chaperonin 60 and chaperonin 10 genes. The two genes are arranged in a head-to-head configuration and together comprise 14 kb and contain 14 introns. The genes are linked together by a region of approximately 280 bp, which constitutes a bidirectional promoter and includes a common heat-shock element. Insertion of the shared promoter region between two reporter genes is sufficient to drive their expression under both constitutive and heat-shock conditions. The arrangement of the mammalian chaperonin genes suggests the potential to provide the coordinated regulation of their products in a manner that is mechanistically distinct from, yet conceptually similar to, that employed by the bacterial chaperonin ( groE) operon.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0378-1119 1879-0038
DOI:	10.1016/S0378-1119(97)00111-X