Search Results - "Sampedro, José G"

Trehalose Mediated Inhibition of Lactate Dehydrogenase from Rabbit Muscle. The Application of Kramers’ Theory in Enzyme Catalysis by Hernández-Meza, Juan M, Sampedro, José G

“…Lactate dehydrogenase (LDH) catalyzes the reduction of pyruvate to lactate by using NADH. LDH kinetics has been proposed to be dependent on the dynamics of a…”
Get full text

Purification and Partial Biochemical Characterization of Polyphenol Oxidase from Mango (Mangifera indica cv. Manila) by Palma-Orozco, Gisela, Marrufo-Hernández, Norma A, Sampedro, José G, Nájera, Hugo

“…Polyphenol oxidase (PPO) is an enzyme widely distributed in the plant kingdom that has been detected in most fruits and vegetables. PPO was extracted and…”
Get full text

Effect of Cations on ATP Binding to the N-domain of Na + , K + -ATPase by Ramírez-Alonso, Jocelin I, Sampedro, José G

“…The nucleotide-binding domain (N-domain) of the Na , K -ATPase (NKA) is physicochemically characterized by a high content of Glu and Asp residues, resulting in…”
Get full text

Bone Regeneration Induced by Strontium Folate Loaded Biohybrid Scaffolds by Martín-Del-Campo, Marcela, Sampedro, José G, Flores-Cedillo, María Lisseth, Rosales-Ibañez, Raul, Rojo, Luis

“…Nowadays, regenerative medicine has paid special attention to research (in vitro and in vivo) related to bone regeneration, specifically in the treatment of…”
Get full text

glycolytic metabolon in Saccharomyces cerevisiae is stabilized by F‐actin by Araiza‐Olivera, Daniela, Chiquete‐Felix, Natalia, Rosas‐Lemus, Mónica, Sampedro, José G, Peña, Antonio, Mujica, Adela, Uribe‐Carvajal, Salvador

“…In the Saccharomyces cerevisiae glycolytic pathway, 11 enzymes catalyze the stepwise conversion of glucose to two molecules of ethanol plus two CO₂ molecules…”
Get full text

Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET by Sampedro, José G, Cataño, Yolanda

“…The sarco/endoplasmic reticulum Ca -ATPase (SERCA) is a P-type ATPase that has been crystallized in various conformations. Detailed functional information may…”
Get more information

Purification and partial biochemical characterization of polyphenol oxidase from mamey ( Pouteria sapota) by Palma-Orozco, Gisela, Ortiz-Moreno, Alicia, Dorantes-Álvarez, Lidia, Sampedro, José G., Nájera, Hugo

“…In this study, two isoenzymes of PPO from mamey fruit were obtained by chromatographic column separation; PPO 1 was obtained before the KCl gradient while PPO…”
Get full text

Isolation of the Sarcoplasmic Reticulum Ca2+-ATPase from Rabbit Fast-Twitch Muscle by Rivera-Morán, Miguel A., Sampedro, José G.

“…The sarcoendoplasmic reticulum Ca2+-ATPase (SERCA) is a membrane protein that is destabilized during purification in the absence of calcium ions. The…”
Get full text

The Oligomeric State of the Plasma Membrane H⁺-ATPase from Kluyveromyces lactis by Ruiz-Granados, Yadira G, De La Cruz-Torres, Valentín, Sampedro, José G

“…The plasma membrane H⁺-ATPase was purified from the yeast . The oligomeric state of the H⁺-ATPase is not known. Size exclusion chromatography displayed two…”
Get full text

ANS Interacts with the Ca2+-ATPase Nucleotide Binding Site by De la Cruz-Torres, Valentín, Cataño, Yolanda, Olivo-Rodríguez, Montserrat, Sampedro, José G.

“…The binding of 8-anilino-1-naphthalene sulfonate (ANS) to the nucleotide binding domain (N-domain) of the sarcoplasmic reticulum Ca 2+ -ATPase (SERCA) was…”
Get full text

Nucleotide Binding in an Engineered Recombinant Ca2+-ATPase N‑Domain by Páez-Pérez, Edgar D, De La Cruz-Torres, Valentín, Sampedro, José G

“…A recombinant Ca2+-ATPase nucleotide binding domain (N-domain) harboring the mutations Trp552Leu and Tyr587Trp was expressed and purified. Chemical…”
Get full text

Kramers’ Theory and the Dependence of Enzyme Dynamics on Trehalose-Mediated Viscosity by José G. Sampedro, Miguel A. Rivera-Moran, Salvador Uribe-Carvajal

“…The disaccharide trehalose is accumulated in the cytoplasm of some organisms in response to harsh environmental conditions. Trehalose biosynthesis and…”
Get full text

In situ Inactivation of Polyphenol Oxidase in Mamey Fruit (Pouteria sapota) by Microwave Treatment by Palma-Orozco, Gisela, Sampedro, José G., Ortiz-Moreno, Alicia, Nájera, Hugo

“…:  Polyphenol oxidase (PPO) is the enzyme responsible for quality loss in most fruits and vegetables. Quality loss is mainly because of oxidative chemical…”
Get full text

Enhanced thermal stability and pH behavior of glucose oxidase on electrostatic interaction with polyethylenimine by Padilla-Martínez, Silvia G., Martínez-Jothar, Lucía, Sampedro, José G., Tristan, Ferdinando, Pérez, Elías

“…Electrostatic interactions, mediated by ionic-exchange, between polyethylenimine (PEI) and glucose oxidase (GOx) were used to form GOx–PEI macro-complex, which…”
Get full text

Trehalose-enzyme interactions result in structure stabilization and activity inhibition. The role of viscosity by Sampedro, José G, Uribe, Salvador

“…Stress resistance is essential for survival. The mechanisms of molecule stabilization during stress are of interest for biotechnology, where many enzymes and…”
Get full text

Trehalose-mediated thermal stabilization of glucose oxidase from Aspergillus niger by Paz-Alfaro, Karina J., Ruiz-Granados, Yadira G., Uribe-Carvajal, Salvador, Sampedro, José G.

“…Thermal inactivation and enzyme kinetics of glucose oxidase (a FAD dependent enzyme) were studied in the absence and presence of trehalose. The inactivation…”
Get full text

Mapping the ATP Binding Site in the Plasma Membrane H+-ATPase from Kluyveromyces lactis by Sampedro, José G., Nájera, Hugo, Uribe-Carvajal, Salvador, Ruiz-Granados, Yadira G.

“…The plasma membrane H + -ATPase from Kluyveromyces lactis contains 14 tryptophan residues. Binding a nucleotide or unfolding with Gnd-HCl quenched intrinsic…”
Get full text

Measuring Solution Viscosity and its Effect on Enzyme Activity by Uribe, Salvador, Sampedro, José G.

“…In proteins, some processes require conformational changes involving structural domain diffusion. Among these processes are protein folding, unfolding and…”
Get full text

association of glycolytic enzymes from yeast confers resistance against inhibition by trehalose by Araiza-Olivera, Daniela, Sampedro, José G, Mújica, Adela, Peña, Antonio, Uribe-Carvajal, Salvador

“…During stress, many organisms accumulate compatible solutes. These solutes must be eliminated upon return to optimal conditions as they inhibit cell metabolism…”
Get full text

Isolation of the Sarcoplasmic Reticulum Ca[sup.2+]-ATPase from Rabbit Fast-Twitch Muscle by Rivera-Morán, Miguel A, Sampedro, José G

“…The sarcoendoplasmic reticulum Ca[sup.2+]-ATPase (SERCA) is a membrane protein that is destabilized during purification in the absence of calcium ions. The…”
Get full text