Anticancer Effects of Vitis vinifera L. Mediated Biosynthesized Silver Nanoparticles and Cotreatment with 5 Fluorouracil on HT-29 Cell Line

Anticancer Effects of Vitis vinifera L. Mediated Biosynthesized Silver Nanoparticles and Cotreatment with 5 Fluorouracil on HT-29 Cell Line

The aim of this study was to evaluate the anticancer effects of biosynthesized silver nanoparticles (Vv-AgNPs) from grape ( Vitis vinifera L. ) seed aqueous extract, alone or in combination with 5-Fluorouracil (5-FU) on HT-29 cell line. Vv-AgNPs were characterized by techniques such as UV–vis spectr...

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Bibliographic Details
Published in:Biological trace element research Vol. 200; no. 7; pp. 3159 - 3170
Main Authors: Salman, Giray, Pehlivanoglu, Suray, Aydin Acar, Cigdem, Yesilot, Sukriye
Format: Journal Article
Language:English
Published: New York Springer US 01-07-2022
Springer Nature B.V
Subjects:
5-Fluorouracil
Analytical methods
Anticancer properties
Antioxidants
Antiproliferatives
Apoptosis
Biochemistry
Biomedical and Life Sciences
Biotechnology
Bromodeoxyuridine
Cancer
Caspase-3
Colorectal cancer
Cytotoxicity
Dosage
Electron microscopy
Life Sciences
Nanoparticles
Nutrition
Oncology
Oxidants
Oxidative stress
Oxidizing agents
p53 Protein
Proteins
Scanning electron microscopy
Silver
Spectrophotometers
Vitis vinifera
Western blotting
X-ray spectroscopy
Green synthesis
HT-29 cell line
Colon cancer
5-Fluorouracil
Silver nanoparticles
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Summary:The aim of this study was to evaluate the anticancer effects of biosynthesized silver nanoparticles (Vv-AgNPs) from grape ( Vitis vinifera L. ) seed aqueous extract, alone or in combination with 5-Fluorouracil (5-FU) on HT-29 cell line. Vv-AgNPs were characterized by techniques such as UV–vis spectrophotometer (surface plasmon peak 454 nm), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX). HT-29 cells were treated with different concentrations (0–80 μg/mL for MTT) and (0–20 μg/mL for BrdU) of Vv-AgNPs alone and combined with (200 μg/mL) 5-FU for 72 h. The cytotoxic effects were analyzed by [3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyl tetrazolium bromide] (MTT) assay (IC 50 values 13.74 and 5.35 μg/mL, respectively). Antiproliferative effects were examined 5-bromo-2'-deoxyuridine (BrdU) assay (IC 50 values 9.65 and 5.00 μg/mL, respectively). Activation of caspase-3 and protein expression levels of p53 were determined by Western blotting analysis. It was observed that Vv-AgNPs significantly increased the cleavage of the proapoptotic proteins caspase 3 and obviously enhanced the expression of p53 in a dose-dependent manner. The increased amount of total oxidant status (TOS) in the 10 μg/mL Vv-AgNPs + 5-FU treatment group, despite the increasing amount of total antioxidant status (TAS), caused an increase in Oxidative Stress Index (OSI) compared to the control. In this study, it has been shown in vitro that the use of successfully biosynthesized Vv-AgNPs in combination with 5-FU exhibits synergistic cytotoxic, antiproliferative, apoptotic, and oxidative effects against HT-29 cell line.
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ISSN:0163-4984
1559-0720
DOI:10.1007/s12011-021-02923-8