Partial characterization of a novel amphibian hemoglobin as a model for graduate student investigation on peptide chemistry, mass spectrometry, and atomic force microscopy

Partial characterization of a novel amphibian hemoglobin as a model for graduate student investigation on peptide chemistry, mass spectrometry, and atomic force microscopy

Graduate students in chemistry, and in biological and biomedical fields must learn the fundamentals and practices of peptide and protein chemistry as early as possible. A project‐oriented approach was conducted by first‐year M.Sc and Ph.D students in biological sciences. A blind glass slide containi...

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Published in:Biochemistry and molecular biology education Vol. 40; no. 2; pp. 121 - 129
Main Authors: Bemquerer, Marcelo P., Macedo, Jéssica K. A., Ribeiro, Ana Carolina J., Carvalho, Andréa C., Silva, Débora O. C., Braz, Juliana M., Medeiros, Kelliane A., Sallet, Lunalva A. P., Campos, Pollyanna F., Prates, Maura V., Silva, Luciano P.
Format: Journal Article
Language:English
Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01-03-2012
Wiley-Blackwell
Subjects:
Amphibians - metabolism
Animals
Anura - metabolism
atomic force microscopy
Biology
Biology - education
Biomedicine
Chemistry
chromatography
College Science
Cytology
Education, Graduate
Graduate Students
hemoglobin
Hemoglobins - chemistry
Laboratory Equipment
mass spectrometry
Mass Spectrometry - methods
Microscopy, Atomic Force
Nuclear Energy
peptide synthesis
Peptides - chemistry
Peptides - isolation & purification
Protein chemistry
red blood cell
Science Instruction
Science Process Skills
Scientific Concepts
Spectroscopy
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Summary:Graduate students in chemistry, and in biological and biomedical fields must learn the fundamentals and practices of peptide and protein chemistry as early as possible. A project‐oriented approach was conducted by first‐year M.Sc and Ph.D students in biological sciences. A blind glass slide containing a cellular smear and an aqueous cellular extract were offered to the students. Qualitative and quantitative cell morphological parameters were analyzed by atomic force microscopy. The fractionation of the aqueous extract was conducted by reversed‐phase chromatography followed by analysis of the isolated and partially purified proteins and peptides by mass spectrometry (MS). The proteins were treated by peptidases and the obtained peptide fragments were sequenced by de novo MS/MS, together with peptides already present in the extract. The most abundant protein fractions were identified as the alpha and beta chains of hemoglobin from an amphibian of the Leptodactylus genera. Two of the peptides sequenced by the students were synthesized by the solid‐phase methodology, one of those being obtained by the split‐and‐pool library synthesis method. Thus, the students were able to learn some advanced principles and practices of protein chemistry and bionanotechnology in a 6‐weeks project‐oriented approach.
Bibliography:M.P.B., J.K.A.M, and L.P.S. wrote the paper. All the authors performed the experiments, discussed the results, and commented on the manuscript.
This work is supported by CAPES, CNPq, and FAPDF.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1470-8175
1539-3429
DOI:10.1002/bmb.20564