Role of Diosmin in protection against the oxidative stress induced damage by gamma-radiation in Wistar albino rats

The benchmark of this study is to evaluate the radio protective efficiency of diosmin, a natural citrus flavone of hesperidin derivative on radiation-induced damage in wistar albino rats. Rats orally administered two diosmin doses (100 and 200 mg/kg body wt.) for a month (every other day) prior to e...

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Published in:	Regulatory toxicology and pharmacology Vol. 113; p. 104622
Main Authors:	Mahgoub, Shahenda, Sallam, Anas O., Sarhan, Hazem K.A., Ammar, Amal A.A., Soror, Sameh H.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier Inc 01-06-2020
Subjects:	Administration, Oral Animals Apoptosis Apoptosis - drug effects Diosmin Diosmin - administration & dosage Diosmin - chemistry Diosmin - pharmacology DNA Damage Dose-Response Relationship, Drug Gamma radiation Gamma Rays Glutathione - metabolism Lipid Peroxidation - drug effects Male Oxidative Stress - drug effects Protective Agents - administration & dosage Protective Agents - chemistry Protective Agents - pharmacology Radioprotection Rats Rats, Wistar Reactive oxygen species Superoxide Dismutase - metabolism Reactive oxygen species Gamma radiation Radioprotection DNA damage Diosmin Apoptosis
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Summary:	The benchmark of this study is to evaluate the radio protective efficiency of diosmin, a natural citrus flavone of hesperidin derivative on radiation-induced damage in wistar albino rats. Rats orally administered two diosmin doses (100 and 200 mg/kg body wt.) for a month (every other day) prior to exposure to high gamma radiation single dose (8Gy) or cumulative dose (10Gy). To evaluate the radio protective efficiency of diosmin various biochemical estimations, histopathological alterations as well as comet assay and caspase-3 activity for assessment of apoptosis were performed. Results indicated that radiation-induced decline in the levels of antioxidant parameters (SOD and GSH), increased lipid peroxidation, DNA damage and apoptosis were improved by pre-administration of diosmin. Diosmin dose (200 mg/kg body wt.) restored the antioxidant status to near normal and reduced lipid peroxidation, DNA and tissue damage. These results were confirmed by histopathological examinations, which showed that pre-administration of diosmin protected the liver and kidney of albino rats against gamma-irradiation induced damage. Hence, it has been illustrated that diosmin might be an effective radio protector against radiation-induced damage in rats. Moreover, diosmin alone pretreated group did not show any biochemical alterations or DNA damage indicating the protective nature of the drug. •Diosmin prevents oxidative stress.•Radiation induced apoptosis was alleviated by pre-administration of diosmin.•Irradiation of rats with single and fractionated doses.•Diosmin showed radioprotective effect in vivo.
ISSN:	0273-2300 1096-0295
DOI:	10.1016/j.yrtph.2020.104622