Molecular detection of beta-lactamase bla CTX-M group 1 in Escherichia coli isolated from drinking water in Khartoum State

This study aimed to detect the bla group 1 in Escherichia coli (E. coli) isolated from drinking water in Khartoum State. Two hundred and eighty water samples were collected randomly from different areas, places, and sources from the state and examined for the presence of E. coli as a fecal contamina...

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Bibliographic Details
Published in:	Journal of water and health Vol. 18; no. 6; p. 1091
Main Authors:	Altayb, Hisham N, Salih, Eman Khalid, Moglad, Ehssan H
Format:	Journal Article
Language:	English
Published:	England 01-12-2020
Subjects:	Anti-Bacterial Agents - pharmacology beta-Lactamases - genetics Drinking Water Escherichia coli Escherichia coli Infections Feces Humans
Online Access:	Get more information
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Summary:	This study aimed to detect the bla group 1 in Escherichia coli (E. coli) isolated from drinking water in Khartoum State. Two hundred and eighty water samples were collected randomly from different areas, places, and sources from the state and examined for the presence of E. coli as a fecal contamination indicator. Isolation and identification of E. coli were performed using culture characteristics on different culture media and biochemical reactions. An antimicrobial sensitivity test was performed for all isolated E. coli using agar disk diffusion method. DNA was extracted by boiling method, and bacterial genomic DNA used as a template to detect bla group 1 by PCR. Results showed 86 (30.7%) E. coli were isolated out of 280 water samples. Antimicrobial susceptibility testing revealed the highest resistant percentage was 59% for tetracycline, followed by 35% for gentamycin, while for chloramphenicol and cefotaxime was 22 and 20%, respectively. bla group 1 was detected in about 40% of all isolates. This study concludes that drinking water in Khartoum State may be contaminated with feces and might be a possible source for transferring resistant bacteria. Thus, it may be one of the critical causes of increasing reports of antimicrobial resistance in Khartoum State.
ISSN:	1477-8920
DOI:	10.2166/wh.2020.160