Leishmania infantum exo-antigens: application toward serological diagnosis of visceral leishmaniasis

Leishmania infantum exo-antigens: application toward serological diagnosis of visceral leishmaniasis

The laboratory diagnosis of visceral leishmaniasis (VL) presents limitations related to its sensibility and/or specificity. In this context, the aim of this study was to evaluate an enzyme-linked immunoassay to detect IgG antibodies against Leishmania infantum exo-antigens for diagnosis of VL, calle...

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Bibliographic Details
Published in:Parasitology research (1987) Vol. 118; no. 7; pp. 2317 - 2323
Main Authors: Saliba, Juliana W., Lopes, Karine F., Silva-Pereira, Rosiane A., Teixeira, Luciana A. S., Oliveira, Edward
Format: Journal Article
Language:English
Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-07-2019
Springer
Springer Nature B.V
Subjects:
Antigens
Biomedical and Life Sciences
Biomedicine
Chagas disease
Cross-reactivity
Diagnosis
Enzyme-linked immunosorbent assay
Enzymes
Immunoglobulin G
Immunology
Kala-azar
Leishmania infantum
Malaria
Medical Microbiology
Microbiology
Parasitic diseases
Protozoology - Short Communication
Schistosomiasis
Tegumentary leishmaniasis
Tropical diseases
Visceral leishmaniasis
Western blotting
Brazil
Canada
ELISA-Exo
Serological tests
Visceral leishmaniasis
Immunoreactive exo-antigens
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Summary:The laboratory diagnosis of visceral leishmaniasis (VL) presents limitations related to its sensibility and/or specificity. In this context, the aim of this study was to evaluate an enzyme-linked immunoassay to detect IgG antibodies against Leishmania infantum exo-antigens for diagnosis of VL, called ELISA-Exo. This assay was applied in 309 masked serum samples from VL, tegumentary leishmaniasis, Chagas disease, schistosomiasis mansoni, malaria patients, and healthy individuals. The results were compared with those from ELISA using rK39 as antigen (ELISA-rK39). The ELISA assays presented sensitivity of 96.8% and 98.4% ( p  = 0.68), specificity of 92.4% for both, and diagnostic accuracy of 94.2% and 94.8% ( p  = 0.48) by the ELISA-Exo and ELISA-rK39, respectively. An excellent agreement beyond chance (Kappa index = 0.82) was obtained when the results from ELISA assays were cross-tabulated. The Western blotting showed that false-positive results presented by ELISA-Exo probably were produced by cross-reactivity of antigens shared with the species of the family Trypanosomatidae. In the future, an immunoproteomic approach can contribute for identification of main immunoreactive L. infantum exo-antigens.
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ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-019-06352-4