GUT MICROSYMBIOCENOSIS IN CHILDREN WITH REACTIVE ARTHRITIS

GUT MICROSYMBIOCENOSIS IN CHILDREN WITH REACTIVE ARTHRITIS

To study the state of gut microsymbiocenosis in children with reactive arthritis (RA), with the assessment of biofilm formation (BFF) of microsymbionts and the ability to change cytokine levels (their anticyokine activity) in vitro. The investigation of gut microsymbiocenosis by means of bacteriolog...

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Published in:Žurnal mikrobiologii, ėpidemiologii i immunobiologii Vol. 93; no. 6; pp. 41 - 48
Main Authors: Bukharin, O V, Chelpachenko, O E, Danilova, E I, Chainikova, I N, Perunova, N B, Ivanova, E V, Nikiforov, I A, Fedotova, L P, Bondarenko, T A, Salgina, A V
Format: Journal Article
Language:English
Russian
Published: Russia (Federation) Central Research Institute for Epidemiology 01-11-2016
Subjects:
Adolescent
anticytokine activity
Arthritis, Juvenile - microbiology
Bacteria - classification
Bacteria - growth & development
Bacteria - isolation & purification
biofilm formation
Candida - classification
Candida - growth & development
Candida - isolation & purification
Child
Child, Preschool
children
Female
Gastrointestinal Microbiome
gut microsymbiocenosis
Humans
Male
reactive arthritis
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Summary:To study the state of gut microsymbiocenosis in children with reactive arthritis (RA), with the assessment of biofilm formation (BFF) of microsymbionts and the ability to change cytokine levels (their anticyokine activity) in vitro. The investigation of gut microsymbiocenosis by means of bacteriological method was conducted in 34 children with RA and 25 relatively healthy 3 - 16 year- old children. Microorganisms were identified with the help of MALDI-TOF mass-spectrometry, anticytokine activity (ACA) of microsymbionts - according to Bukharin O.V et al. (2011), biofilm formation - according to O'Toole G.A., Kolter R. (1998). On the ground of species composition differences of gut microbiota discrimination model was created which allowed to separate the group of children with RA from healthy individuals. Microsymbiocenosis of patients with RA was characterized by increasing number of opportunistic microorganisms (OM) (enterobacteria, clostridia, bacteroides, and Candida), BFF and ACA level. The obtained data greatly contribute.to the deciphering of spondylo- arthritis and disclose the role of microbial factor under given pathology. Hypercolonisation of human gut with OM, having pronounced ability to BFF and regulating cytokine level, promotes strengthening of arthritogenic potential and serves as additional marker of arthritis development risk in children.
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ISSN:0372-9311
2686-7613
DOI:10.36233/0372-9311-2016-6-41-48